US5705187A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Compositions of lipids and stabilizing materials
Assignee: ImaRx Therapeutics Inc
•
Filing: 1995-04-05
•
Granted: 1998-01-06
•
Expires: 2015-04-05
Abstract
Abstract Compositions comprising, in an aqueous carrier, a lipid and a material which is capable of stabilizing the composition. The stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat the lipid but insufficient to raise the viscosity of the composition. The compositions are particularly suitable for use in diagnostic applications, including ultrasound. The compositions can take the form of vesicular compositions, such as micelles and liposomes.
Inventors
- 👤 Evan C. Unger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 01:15
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K41/00; A61K41/0028; A61K41/0052; A61K47/00; A61K47/50; A61K47/69; A61K47/6905; A61K47/6907; A61K47/6911; A61K47/6921; A61K47/6925; A61K49/00; A61K49/06; A61K49/18; A61K49/1806; A61K49/1815; A61K49/22; A61K49/222; A61K49/223; A61K49/227; A61K9/1271; A61K9/1277; A61K9/1278; A61M; A61M5/00; A61M5/178; A61M5/31; A61M5/3145; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984
Claims Analysis
Total Claims: 229
Claim 1
Independent Claim
89 words
A vesicular composition comprising, in an aqueous carrier, vesicles comprising a lipid, a gas or gaseous precursor encapsulated in the vesicles, and a material which is capable of stabilizing the composition, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 2
Dependent Claim
89 words
A vesicular composition comprising, in an aqueous carrier, vesicles comprising a lipid, a gas or gaseous precursor encapsulated in the vesicles, and a material which is capable of stabilizing the composition, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 3
Dependent Claim
12 words
A composition according to claim 1 wherein said lipid comprises a phospholipid.
Claim 4
Dependent Claim
21 words
A composition according to claim 2 wherein said phospholipid is selected from the group consisting of phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid.
Claim 5
Dependent Claim
21 words
A composition according to claim 3 wherein said phosphatidylcholine is selected from the group consisting of dioleoylphosphatidylcholine, dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine and distearoylphosphatidylcholine.
Claim 6
Dependent Claim
11 words
A composition according to claim 4 wherein said phosphatidylcholine comprises dipalmitoylphosphatidylcholine.
Claim 7
Dependent Claim
21 words
A composition according to claim 3 wherein said phosphatidylethanolamine is selected from the group consisting of dipalmitoylphosphatidylethanolamine, dioleoylphosphatidylethanolamine, N-succinyldioleoylphosphatidylethanolamine and 1-hexadecyl-2-palmitoylglycerophosphoethanolamine.
Claim 8
Dependent Claim
11 words
A composition according to claim 6 wherein said phosphatidylethanolamine is dipalmitoylphosphatidylethanolamine.
Claim 9
Dependent Claim
13 words
A composition according to claim 3 wherein said phosphatidic acid comprises dipalmitolylphosphatidic acid.
Claim 10
Dependent Claim
22 words
A composition according to claim 1 wherein said polymer is selected from the group consisting of polyhydroxy, polyamine, polycarboxy and polysaccharide polymers.
Claim 11
Dependent Claim
17 words
A composition according to claim 1 which is selected from the group consisting of micelles and liposomes.
Claim 12
Dependent Claim
23 words
A composition according to claim 1 wherein said stabilizing material is present in an amount sufficient to lower the viscosity of the composition.
Claim 13
Dependent Claim
26 words
A composition according to claim 1 wherein said non-covalent association is selected from the group consisting of ionic interaction, dipole--dipole interaction and van der Waals forces.
Claim 14
Dependent Claim
11 words
A composition according to claim 1 further comprising a bioactive agent.
Claim 15
Dependent Claim
22 words
A vesicular composition according to claim 1 further comprising a polymer which is conjugated to at least a portion of said lipid.
Claim 16
Dependent Claim
26 words
A vesicular composition according to claim 14 wherein said polymer is selected from the group consisting of poly(ethylene glycol) (PEG), poly(vinylpyrrolidone), polyoxomers, polysorbate and polyvinyl alcohol.
Claim 17
Dependent Claim
24 words
A vesicular composition according to claim 15 wherein said PEG is selected from the group consisting of PEG 2,000, PEG 5,000 and PEG 8,000.
Claim 18
Dependent Claim
14 words
A vesicular composition according to claim 14 wherein said lipid-polymer conjugate is dipalmitoylphosphatidylethanolamine-PEG 5,000.
Claim 19
Dependent Claim
95 words
A formulation for therapeutic or diagnostic use comprising, in combination with a bioactive agent, vesicles comprising a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the formulation, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the formulation, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 20
Dependent Claim
95 words
A formulation for therapeutic or diagnostic use comprising, in combination with a bioactive agent, vesicles comprising a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the formulation, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the formulation, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 21
Dependent Claim
19 words
A formulation according to claim 18 wherein said vesicles are selected from the group consisting of micelles and liposomes.
Claim 22
Dependent Claim
18 words
A formulation according to claim 19 wherein said bioactive agent is substantially entrapped within said micelles or liposomes.
Claim 23
Dependent Claim
106 words
A process for the preparation of a stabilized vesicular composition comprising vesicles comprising a lipid, a gas or gaseous precursor encapsulated in the vesicles, and a stabilizing material which is capable of associating non-covalently with said lipid, wherein the process comprises combining together said lipid, gas or gaseous precursor, and stabilizing material, wherein said stabilizing material is combined with said lipid in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 24
Dependent Claim
106 words
A process for the preparation of a stabilized vesicular composition comprising vesicles comprising a lipid, a gas or gaseous precursor encapsulated in the vesicles, and a stabilizing material which is capable of associating non-covalently with said lipid, wherein the process comprises combining together said lipid, gas or gaseous precursor, and stabilizing material, wherein said stabilizing material is combined with said lipid in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 25
Dependent Claim
19 words
A process according to claim 21 wherein said composition is selected from the group consisting of micelles and liposomes.
Claim 26
Dependent Claim
113 words
A process for the preparation of a formulation for diagnostic or therapeutic use comprising, in combination with a bioactive agent, a vesicular composition which comprises a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a stabilizing material which is associated non-covalently with the lipid, wherein the process comprises combining together said bioactive agent and vesicular composition, wherein said stabilizing material is present in said composition in an amount sufficient to coat said lipid but insufficient to raise the viscosity of said composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 27
Dependent Claim
113 words
A process for the preparation of a formulation for diagnostic or therapeutic use comprising, in combination with a bioactive agent, a vesicular composition which comprises a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a stabilizing material which is associated non-covalently with the lipid, wherein the process comprises combining together said bioactive agent and vesicular composition, wherein said stabilizing material is present in said composition in an amount sufficient to coat said lipid but insufficient to raise the viscosity of said composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 28
Dependent Claim
19 words
A process according to claim 23 wherein said composition is selected from the group consisting of micelles and liposomes.
Claim 29
Dependent Claim
100 words
A stabilized vesicular composition comprising a lipid, a gas or gaseous precursor encapsulated in the vesicles, and a stabilizing material which is capable of associating non-covalently with said lipid, wherein the composition is prepared by combining together said lipid, gas or gaseous precursor, and stabilizing material, wherein said stabilizing material is combined with said lipid in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 30
Dependent Claim
100 words
A stabilized vesicular composition comprising a lipid, a gas or gaseous precursor encapsulated in the vesicles, and a stabilizing material which is capable of associating non-covalently with said lipid, wherein the composition is prepared by combining together said lipid, gas or gaseous precursor, and stabilizing material, wherein said stabilizing material is combined with said lipid in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 31
Dependent Claim
114 words
A stabilized formulation for diagnostic or therapeutic use comprising a bioactive agent and a vesicular composition which comprises a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the formulation and is associated non-covalently with said lipid, wherein said composition is prepared by combining together said lipid, gas or gaseous precursor and stabilizing material, wherein said stabilizing material is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the formulation, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 32
Dependent Claim
114 words
A stabilized formulation for diagnostic or therapeutic use comprising a bioactive agent and a vesicular composition which comprises a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the formulation and is associated non-covalently with said lipid, wherein said composition is prepared by combining together said lipid, gas or gaseous precursor and stabilizing material, wherein said stabilizing material is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the formulation, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 33
Independent Claim
124 words
A method for providing an image of an internal region of a patient comprising (i) administering to the patient a vesicular composition comprising, in an aqueous carrier, vesicles comprising a lipid, a gas or a gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the composition, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer; and (ii) scanning the patient using ultrasound to obtain a visible image of the region.
Claim 34
Independent Claim
124 words
A method for providing an image of an internal region of a patient comprising (i) administering to the patient a vesicular composition comprising, in an aqueous carrier, vesicles comprising a lipid, a gas or a gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the composition, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer; and (ii) scanning the patient using ultrasound to obtain a visible image of the region.
Claim 35
Independent Claim
127 words
A method for diagnosing the presence of diseased tissue in a patient comprising (i) administering to the patient a vesicular composition comprising, in an aqueous carrier, vesicles comprising a lipid, a gas or a gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the composition, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer; and (ii) scanning the patient using ultrasound to obtain a visible image of any diseased tissue in the patient.
Claim 36
Independent Claim
127 words
A method for diagnosing the presence of diseased tissue in a patient comprising (i) administering to the patient a vesicular composition comprising, in an aqueous carrier, vesicles comprising a lipid, a gas or a gaseous precursor encapsulated in said vesicles, and a material which is capable of stabilizing the composition, wherein said stabilizing material is associated non-covalently with said lipid and is present in an amount sufficient to coat said lipid but insufficient to raise the viscosity of the composition, wherein said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer; and (ii) scanning the patient using ultrasound to obtain a visible image of any diseased tissue in the patient.
Claim 37
Independent Claim
116 words
A method for the therapeutic delivery in vivo of a bioactive agent comprising administering to a patient a therapeutically effective amount of a formulation which comprises, in combination with a bioactive agent, a vesicular composition comprising a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a material which stabilizes said composition, wherein said stabilizing material is associated noncovalently with said lipid and is present in said composition in an amount sufficient to coat said lipid but insufficient to raise the viscosity of said composition, wherein said said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 38
Independent Claim
116 words
A method for the therapeutic delivery in vivo of a bioactive agent comprising administering to a patient a therapeutically effective amount of a formulation which comprises, in combination with a bioactive agent, a vesicular composition comprising a lipid, a gas or gaseous precursor encapsulated in said vesicles, and a material which stabilizes said composition, wherein said stabilizing material is associated noncovalently with said lipid and is present in said composition in an amount sufficient to coat said lipid but insufficient to raise the viscosity of said composition, wherein said said gas and gaseous precursor comprise a fluorinated compound selected from the group consisting of perfluorocarbons and sulfur hexafluoride, and wherein said stabilizing material comprises a polymer.
Claim 39
Dependent Claim
13 words
A composition according to claim 1 wherein said fluorinated compound is sulfur hexafluoride.
Claim 40
Dependent Claim
13 words
A composition according to claim 1 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 41
Dependent Claim
21 words
A composition according to claim 1 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 42
Dependent Claim
12 words
A composition according to claim 32 wherein said vesicles comprise unilamellar vesicles.
Claim 43
Dependent Claim
12 words
A composition according to claim 33 wherein said vesicles comprise a monolayer.
Claim 44
Dependent Claim
18 words
A composition according to claim 34 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 45
Dependent Claim
18 words
A composition according to claim 34 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 46
Dependent Claim
19 words
A composition according to claim 34 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 47
Dependent Claim
12 words
A composition according to claim 33 wherein said vesicles comprise a bilayer.
Claim 48
Dependent Claim
18 words
A composition according to claim 38 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 49
Dependent Claim
18 words
A composition according to claim 38 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 50
Dependent Claim
19 words
A composition according to claim 38 whereto said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 51
Dependent Claim
20 words
A composition according to claim 32 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 52
Dependent Claim
12 words
A composition according to claim 42 wherein said vesicles comprise a monolayer.
Claim 53
Dependent Claim
18 words
A composition according to claim 43 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 54
Dependent Claim
18 words
A composition according to claim 43 whereto said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 55
Dependent Claim
19 words
A composition according to claim 43 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 56
Dependent Claim
12 words
A composition according to claim 42 wherein said vesicles comprise a bilayer.
Claim 57
Dependent Claim
18 words
A composition according to claim 47 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 58
Dependent Claim
18 words
A composition according to claim 47 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 59
Dependent Claim
19 words
A composition according to claim 47 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 60
Dependent Claim
13 words
A formulation according to claim 18 wherein said fluorinated compound is sulfur hexafluoride.
Claim 61
Dependent Claim
13 words
A formulation according to claim 18 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 62
Dependent Claim
21 words
A formulation according to claim 18 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 63
Dependent Claim
12 words
A formulation according to claim 53 wherein said vesicles comprise unilamellar vesicles.
Claim 64
Dependent Claim
12 words
A formulation according to claim 54 wherein said vesicles comprise a monolayer.
Claim 65
Dependent Claim
18 words
A formulation according to claim 55 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 66
Dependent Claim
18 words
A formulation according to claim 55 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 67
Dependent Claim
19 words
A formulation according to claim 55 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 68
Dependent Claim
12 words
A formulation according to claim 54 wherein said vesicles comprise a bilayer.
Claim 69
Dependent Claim
18 words
A formulation according to claim 59 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 70
Dependent Claim
18 words
A formulation according to claim 59 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 71
Dependent Claim
19 words
A formulation according to claim 59 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 72
Dependent Claim
20 words
A formulation according to claim 53 whereto said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 73
Dependent Claim
12 words
A formulation according to claim 63 wherein said vesicles comprise a monolayer.
Claim 74
Dependent Claim
18 words
A formulation according to claim 64 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 75
Dependent Claim
18 words
A formulation according to claim 64 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 76
Dependent Claim
19 words
A formulation according to claim 64 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 77
Dependent Claim
12 words
A formulation according to claim 63 wherein said vesicles comprise a bilayer.
Claim 78
Dependent Claim
18 words
A formulation according to claim 68 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 79
Dependent Claim
18 words
A formulation according to claim 68 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 80
Dependent Claim
19 words
A formulation according to claim 68 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 81
Dependent Claim
13 words
A process according to claim 21 wherein said fluorinated compound is sulfur hexafluoride.
Claim 82
Dependent Claim
13 words
A process according to claim 21 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 83
Dependent Claim
21 words
A process according to claim 21 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 84
Dependent Claim
12 words
A process according to claim 74 wherein said vesicles comprise unilamellar vesicles.
Claim 85
Dependent Claim
12 words
A process according to claim 75 wherein said vesicles comprise a monolayer.
Claim 86
Dependent Claim
18 words
A process according to claim 76 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 87
Dependent Claim
18 words
A process according to claim 76 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 88
Dependent Claim
19 words
A process according to claim 76 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 89
Dependent Claim
12 words
A process according to claim 75 wherein said vesicles comprise a bilayer.
Claim 90
Dependent Claim
18 words
A process according to claim 80 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 91
Dependent Claim
18 words
A process according to claim 80 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 92
Dependent Claim
19 words
A process according to claim 80 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 93
Dependent Claim
20 words
A process according to claim 74 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 94
Dependent Claim
12 words
A process according to claim 84 wherein said vesicles comprise a monolayer.
Claim 95
Dependent Claim
18 words
A process according to claim 85 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 96
Dependent Claim
18 words
A process according to claim 85 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 97
Dependent Claim
19 words
A process according to claim 85 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 98
Dependent Claim
12 words
A process according to claim 84 wherein said vesicles comprise a bilayer.
Claim 99
Dependent Claim
18 words
A process according to claim 89 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 100
Dependent Claim
18 words
A process according to claim 89 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 101
Dependent Claim
19 words
A process according to claim 89 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 102
Dependent Claim
13 words
A process according to claim 23 whereto said fluorinated compound is sulfur hexafluoride.
Claim 103
Dependent Claim
13 words
A process according to claim 23 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 104
Dependent Claim
21 words
A process according to claim 23 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 105
Dependent Claim
12 words
A process according to claim 95 wherein said vesicles comprise unilamellar vesicles.
Claim 106
Dependent Claim
12 words
A process according to claim 95 wherein said vesicles comprise a monolayer.
Claim 107
Dependent Claim
18 words
A process according to claim 97 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 108
Dependent Claim
18 words
A process according to claim 97 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 109
Dependent Claim
19 words
A process according to claim 97 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 110
Dependent Claim
12 words
A process according to claim 96 wherein said vesicles comprise a bilayer.
Claim 111
Dependent Claim
18 words
A process according to claim 101 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 112
Dependent Claim
18 words
A process according to claim 101 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 113
Dependent Claim
19 words
A process according to claim 101 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 114
Dependent Claim
20 words
A process according to claim 95 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 115
Dependent Claim
12 words
A process according to claim 105 wherein said vesicles comprise a monolayer.
Claim 116
Dependent Claim
18 words
A process according to claim 106 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 117
Dependent Claim
18 words
A process according to claim 106 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 118
Dependent Claim
19 words
A process according to claim 106 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 119
Dependent Claim
12 words
A process according to claim 105 wherein said vesicles comprise a bilayer.
Claim 120
Dependent Claim
18 words
A process according to claim 110 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 121
Dependent Claim
18 words
A process according to claim 110 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 122
Dependent Claim
19 words
A process according to claim 110 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 123
Dependent Claim
13 words
A composition according to claim 25 wherein said fluorinated compound is sulfur hexafluoride.
Claim 124
Dependent Claim
13 words
A composition according to claim 25 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 125
Dependent Claim
21 words
A composition according to claim 25 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 126
Dependent Claim
12 words
A composition according to claim 116 wherein said vesicles comprise unilamellar vesicles.
Claim 127
Dependent Claim
12 words
A composition according to claim 117 wherein said vesicles comprise a monolayer.
Claim 128
Dependent Claim
18 words
A composition according to claim 118 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 129
Dependent Claim
18 words
A composition according to claim 118 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 130
Dependent Claim
19 words
A composition according to claim 118 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 131
Dependent Claim
12 words
A composition according to claim 117 wherein said vesicles comprise a bilayer.
Claim 132
Dependent Claim
18 words
A composition according to claim 122 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 133
Dependent Claim
18 words
A composition according to claim 122 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 134
Dependent Claim
19 words
A composition according to claim 122 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 135
Dependent Claim
20 words
A composition according to claim 116 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 136
Dependent Claim
12 words
A composition according to claim 126 wherein said vesicles comprise a monolayer.
Claim 137
Dependent Claim
18 words
A composition according to claim 126 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 138
Dependent Claim
18 words
A composition according to claim 126 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 139
Dependent Claim
19 words
A composition according to claim 126 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 140
Dependent Claim
12 words
A composition according to claim 126 wherein said vesicles comprise a bilayer.
Claim 141
Dependent Claim
18 words
A composition according to claim 131 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 142
Dependent Claim
18 words
A composition according to claim 131 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 143
Dependent Claim
19 words
A composition according to claim 131 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 144
Dependent Claim
13 words
A formulation according to claim 26 wherein said fluorinated compound is sulfur hexafluoride.
Claim 145
Dependent Claim
13 words
A formulation according to claim 26 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 146
Dependent Claim
21 words
A formulation according to claim 26 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 147
Dependent Claim
12 words
A formulation according to claim 137 wherein said vesicles comprise unilamellar vesicles.
Claim 148
Dependent Claim
12 words
A formulation according to claim 138 wherein said vesicles comprise a monolayer.
Claim 149
Dependent Claim
18 words
A formulation according to claim 138 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 150
Dependent Claim
18 words
A formulation according to claim 138 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 151
Dependent Claim
19 words
A formulation according to claim 138 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 152
Dependent Claim
12 words
A formulation according to claim 137 wherein said vesicles comprise a bilayer.
Claim 153
Dependent Claim
18 words
A formulation according to claim 143 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 154
Dependent Claim
18 words
A formulation according to claim 143 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 155
Dependent Claim
19 words
A formulation according to claim 143 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 156
Dependent Claim
20 words
A formulation according to claim 137 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 157
Dependent Claim
12 words
A formulation according to claim 147 wherein said vesicles comprise a monolayer.
Claim 158
Dependent Claim
18 words
A formulation according to claim 148 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 159
Dependent Claim
18 words
A formulation according to claim 148 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 160
Dependent Claim
19 words
A formulation according to claim 148 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 161
Dependent Claim
12 words
A formulation according to claim 14 wherein said vesicles comprise a bilayer.
Claim 162
Dependent Claim
18 words
A formulation according to claim 152 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 163
Dependent Claim
18 words
A formulation according to claim 152 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 164
Dependent Claim
19 words
A formulation according to claim 152 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 165
Dependent Claim
13 words
A method according to claim 27 wherein said fluorinated compound is sulfur hexafluoride.
Claim 166
Dependent Claim
13 words
A method according to claim 27 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 167
Dependent Claim
21 words
A method according to claim 27 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 168
Dependent Claim
12 words
A method according to claim 158 wherein said vesicles comprise unilamellar vesicles.
Claim 169
Dependent Claim
12 words
A method according to claim 159 wherein said vesicles comprise a monolayer.
Claim 170
Dependent Claim
18 words
A method according to claim 160 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 171
Dependent Claim
18 words
A method according to claim 160 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 172
Dependent Claim
19 words
A method according to claim 160 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 173
Dependent Claim
12 words
A method according to claim 159 wherein said vesicles comprise a bilayer.
Claim 174
Dependent Claim
18 words
A method according to claim 164 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 175
Dependent Claim
17 words
A method according to claim 164 wherein said lipid is a phospholipid and fluorinated compound is perfluoropropane.
Claim 176
Dependent Claim
19 words
A method according to claim 164 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 177
Dependent Claim
20 words
A method according to claim 158 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 178
Dependent Claim
12 words
A method according to claim 168 wherein said vesicles comprise a monolayer.
Claim 179
Dependent Claim
18 words
A method according to claim 169 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 180
Dependent Claim
18 words
A method according to claim 169 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 181
Dependent Claim
19 words
A method according to claim 169 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 182
Dependent Claim
12 words
A method according to claim 168 wherein said vesicles comprise a bilayer.
Claim 183
Dependent Claim
18 words
A method according to claim 173 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 184
Dependent Claim
18 words
A method according to claim 173 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 185
Dependent Claim
19 words
A method according to claim 173 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 186
Dependent Claim
13 words
A method according to claim 28 wherein said fluorinated compound is sulfur hexafluoride.
Claim 187
Dependent Claim
13 words
A method according to claim 28 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 188
Dependent Claim
21 words
A method according to claim 28 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 189
Dependent Claim
12 words
A method according to claim 179 wherein said vesicles comprise unilamellar vesicles.
Claim 190
Dependent Claim
12 words
A method according to claim 180 wherein said vesicles comprise a monolayer.
Claim 191
Dependent Claim
18 words
A method according to claim 181 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 192
Dependent Claim
18 words
A method according to claim 181 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 193
Dependent Claim
19 words
A method according to claim 181 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 194
Dependent Claim
12 words
A method according to claim 180 wherein said vesicles comprise a bilayer.
Claim 195
Dependent Claim
18 words
A method according to claim 185 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 196
Dependent Claim
18 words
A method according to claim 185 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 197
Dependent Claim
19 words
A method according to claim 185 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 198
Dependent Claim
20 words
A method according to claim 179 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 199
Dependent Claim
12 words
A method according to claim 189 wherein said vesicles comprise a monolayer.
Claim 200
Dependent Claim
18 words
A method according to claim 190 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 201
Dependent Claim
18 words
A method according to claim 190 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 202
Dependent Claim
19 words
A method according to claim 190 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 203
Dependent Claim
12 words
A method according to claim 189 wherein said vesicles comprise a bilayer.
Claim 204
Dependent Claim
18 words
A method according to claim 194 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 205
Dependent Claim
18 words
A method according to claim 194 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 206
Dependent Claim
19 words
A method according to claim 194 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 207
Dependent Claim
13 words
A method according to claim 29 wherein said fluorinated compound is sulfur hexafluoride.
Claim 208
Dependent Claim
13 words
A method according to claim 29 wherein said fluorinated compound comprises a perfluorocarbon.
Claim 209
Dependent Claim
21 words
A method according to claim 29 wherein said vesicles are selected from the group consisting of unilamellar, oligolamellar and multilamellar vesicles.
Claim 210
Dependent Claim
12 words
A method according to claim 200 wherein said vesicles comprise unilamellar vesicles.
Claim 211
Dependent Claim
12 words
A method according to claim 201 wherein said vesicles comprise a monolayer.
Claim 212
Dependent Claim
18 words
A method according to claim 202 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 213
Dependent Claim
18 words
A method according to claim 202 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 214
Dependent Claim
19 words
A method according to claim 202 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 215
Dependent Claim
12 words
A method according to claim 201 wherein said vesicles comprise a bilayer.
Claim 216
Dependent Claim
18 words
A method according to claim 206 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 217
Dependent Claim
18 words
A method according to claim 206 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 218
Dependent Claim
19 words
A method according to claim 206 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 219
Dependent Claim
20 words
A method according to claim 200 wherein said vesicles are selected from the group consisting of oligolamellar and multilamellar vesicles.
Claim 220
Dependent Claim
12 words
A method according to claim 210 wherein said vesicles comprise a monolayer.
Claim 221
Dependent Claim
18 words
A method according to claim 211 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 222
Dependent Claim
18 words
A method according to claim 211 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 223
Dependent Claim
19 words
A method according to claim 211 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 224
Dependent Claim
12 words
A method according to claim 210 wherein said vesicles comprise a bilayer.
Claim 225
Dependent Claim
18 words
A method according to claim 215 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropentane.
Claim 226
Dependent Claim
18 words
A method according to claim 215 wherein said lipid is a phospholipid and said fluorinated compound is perfluoropropane.
Claim 227
Dependent Claim
19 words
A method according to claim 215 wherein said lipid is a phospholipid and said fluorinated compound is sulfur hexafluoride.
Claim 228
Dependent Claim
22 words
A composition according to claim 31 wherein said perfluorocarbon is selected from the group consisting of perfluoromethane, perfluoroethane, perfluoropropane, perfluorobutane and perfluorocyclobutane.
Claim 229
Dependent Claim
22 words
A formulation according to claim 18 wherein said perfluorocarbon is selected from the group consisting of perfluoromethane, perfluoroethane, perfluoropropane, perfluorobutane and perfluorocyclobutane.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-01-05 | 2001-07-07 | 2002-01-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-01-05 | 2005-07-07 | 2006-01-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-01-05 | 2009-07-07 | 2010-01-06 | $7700.00 | Window Closed / Lapsed |