US4675310A
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Liposome composition as gas transport agents
Assignee: Abbott Vascular Devices Ltd
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Filing: 1985-03-25
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Granted: 1987-06-23
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Expires: 2005-03-25
Abstract
Abstract The invention herein involves the use of ligands and phospholipid liposomes as a carrier for the transport of various gases.
Inventors
- 👤 Dennis Chapman
- 👤 James A. Hayward
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:16
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; A61K38/41; A61K38/42; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1273
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
186 words
An aqueous dispersion of liposomal phospholipid polymers wherein the liposome contains a ligand that will reversibly bind with molecular oxygen or with nitrogen or carbon monoxide or carbon dioxide and wherein the liposome is formed from an intermolecularly and/or intramolecularly cross-linked polymer of a conjugated di-yne of the general formula: ##STR9## wherein at least one of B 1 and B 2 is a group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 wherein p is 0 or 1, X 1 is a direct bond or a divalent aliphatic or cycloaliphatic group, Y 1 is H or a monovalent aliphatic or cycloaliphatic group, the total number of carbon atoms in X 1 and Y 1 in each B 1 and/or B 2 being 8 to 26, and the other of B 1 and B 2 is either (a) the same or a different group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 or (b) is an aliphatic or cycloalkphatic group containing at least 8 carbon atoms; n is 0 or 1, m is 2, 3 or 4 and each R independently represents an alkyl group containing 1 to 4 carbon atoms.
Claim 2
Dependent Claim
186 words
An aqueous dispersion of liposomal phospholipid polymers wherein the liposome contains a ligand that will reversibly bind with molecular oxygen or with nitrogen or carbon monoxide or carbon dioxide and wherein the liposome is formed from an intermolecularly and/or intramolecularly cross-linked polymer of a conjugated di-yne of the general formula: ##STR9## wherein at least one of B 1 and B 2 is a group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 wherein p is 0 or 1, X 1 is a direct bond or a divalent aliphatic or cycloaliphatic group, Y 1 is H or a monovalent aliphatic or cycloaliphatic group, the total number of carbon atoms in X 1 and Y 1 in each B 1 and/or B 2 being 8 to 26, and the other of B 1 and B 2 is either (a) the same or a different group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 or (b) is an aliphatic or cycloalkphatic group containing at least 8 carbon atoms; n is 0 or 1, m is 2, 3 or 4 and each R independently represents an alkyl group containing 1 to 4 carbon atoms.
Claim 3
Dependent Claim
17 words
An aqueous dispersion according to claim 1 wherein n=1, m=2 and each R is CH 3 .
Claim 4
Dependent Claim
24 words
An aqueous dispersion according to claim 1 wherein B 1 and B 2 each is an identical --(CO) p --X 1 --C.tbd.C--C.tbd.C--Y 1 group.
Claim 5
Dependent Claim
35 words
An aqueous dispersion according to claim 3 wherein in B 1 and B 2 each p is 1 and there are 16-26 carbon atoms in B 1 and 16-26 carbon atoms in B 2 .
Claim 6
Dependent Claim
20 words
An aqueous dispersion according to claim 1 wherein both of B 1 and B 2 are straight chain aliphatic groups.
Claim 7
Dependent Claim
28 words
An aqueous dispersion according to claim 1 wherein B 1 and B 2 each is a group of formula: --CO--(CH.sub.2).sub.8 --C.tbd.C--C.tbd.C--(CH.sub.2).sub.n CH.sub.3 where n is 9 or 13.
Claim 8
Dependent Claim
50 words
An aqueous dispersion according to claim 1 wherein one of B 1 and B 2 is a group of formula: --CO--(CH.sub.2).sub.8 --C.tbd.C--C.tbd.C--(CH.sub.2).sub.9 CH.sub.3 and the other of B 1 and B 2 is a mixture of --(CH 2 ) 15 --CH 3 and --(CH 2 ) 17 CH 3 groups.
Claim 9
Dependent Claim
26 words
An aqueous dispersion according to claim 1 wherein the ligand is deoxyhaemoglobin or haemoglobin and the dispersed liposomes are capable of reversibly binding with molecular oxygen.
Claim 10
Dependent Claim
28 words
An aqueous dispersion according to claim 1 wherein the liposome is formed from a mixture of the conjugated di-yne I together with cholesterol or a negatively charged phospholipid.
Claim 11
Dependent Claim
27 words
An aqueous dispersion according to claim 1 wherein the liposome is formed from a mixture of the conjugated di-yne I together with alpha-tocopherol and/or butyl hydroxy toluene.
Claim 12
Dependent Claim
26 words
An aqueous dispersion according to claim 1 wherein the liposome is formed from a mixture of the conjugated di-yne I together with a phosphate and/or carbohydrate.
Claim 13
Dependent Claim
11 words
An aqueous dispersion according to claim 1 additionally containing a carbohydrate.
Claim 14
Dependent Claim
37 words
A solid polymer, forming on rehydration an aqueous dispersion capable of reversible binding molecular oxygen or nitrogen or carbon monoxide or carbon dioxide, which is obtained by removing water from an aqueous dispersion according to claim 12.
Claim 15
Dependent Claim
15 words
A solid polymer according to claim 13 wherein the carbohydrate is trehalose, galactose or glucose.
Claim 16
Independent Claim
200 words
A method of producing an aqueous dispersion of liposomal phospholipid polymers by forming an aqueous dispersion comprising liposomes of a conjugated di-yne I ##STR10## wherein at least one of B 1 and B 2 is a group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 wherein p is 0 or 1, X 1 is a direct bond or a divalent aliphatic or cycloaliphatic group, Y 1 is H or a monovalent aliphatic or cycloaliphatic group, the total number of carbon atoms in X 1 and Y 1 in each B 1 and/or B 2 being 8 to 26, and the other of B 1 and B 2 is either (a) the same or a different group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 or (b) is an aliphatic or cycloaliphatic group containing at least 8 carbon atoms; n is 0 or 1, m is 2, 3 or 4 and each R independently represents an alkyl group containing 1 to 4 carbon atoms; introducing a ligand that will reversibly bind with molecular oxygen or with nitrogen or with carbon monoxide or carbon dioxide, the ligand being introduced before, during or after the conjugated di-yne is formed into liposomes, and finally polymerising the conjugated di-yne.
Claim 17
Independent Claim
200 words
A method of producing an aqueous dispersion of liposomal phospholipid polymers by forming an aqueous dispersion comprising liposomes of a conjugated di-yne I ##STR10## wherein at least one of B 1 and B 2 is a group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 wherein p is 0 or 1, X 1 is a direct bond or a divalent aliphatic or cycloaliphatic group, Y 1 is H or a monovalent aliphatic or cycloaliphatic group, the total number of carbon atoms in X 1 and Y 1 in each B 1 and/or B 2 being 8 to 26, and the other of B 1 and B 2 is either (a) the same or a different group of the formula: --(CO).sub.p --X.sub.1 --C.tbd.C--C.tbd.C--Y.sub.1 or (b) is an aliphatic or cycloaliphatic group containing at least 8 carbon atoms; n is 0 or 1, m is 2, 3 or 4 and each R independently represents an alkyl group containing 1 to 4 carbon atoms; introducing a ligand that will reversibly bind with molecular oxygen or with nitrogen or with carbon monoxide or carbon dioxide, the ligand being introduced before, during or after the conjugated di-yne is formed into liposomes, and finally polymerising the conjugated di-yne.
Claim 18
Dependent Claim
16 words
A method according to claim 15 wherein the conjugated di-yne is formed into liposomes by sonication.
Claim 19
Dependent Claim
15 words
A method according to claim 16 wherein multi-lamellar or small diameter unilamellar liposomes are formed.
Claim 20
Dependent Claim
43 words
A method according to claim 15 wherein the ligand is introduced into the dispersion after liposome formation and the dispersion containing the ligand and liposomes is subjected to at least two cycles of freezing and thawing to incorporate the ligand in the liposomes.
Claim 21
Dependent Claim
18 words
A method according to any claim 15 wherein the conjugated di-yne is polymerised by exposure to actinic radiation.
Claim 22
Dependent Claim
48 words
A method according to claim 15 wherein the dispersion additionally contains a carbohydrate and wherein the water is removed from the dispersion to leave a solid polymer which, on rehydration, gives an aqueous dispersion capable of reversibly binding molecular oxygen or nitrogen or carbon monoxide or carbon dioxide.
Claim 23
Dependent Claim
14 words
A method according to claim 20 wherein the carbohydrate is trehalose, galactose or glucose.
Claim 24
Dependent Claim
65 words
A method of transporting a gas which is molecular oxygen, nitrogen, carbon monoxide or carbon dioxide which comprises bringing the gas into contact with an aqueous dispersion according to claim 1 at a first station to form a gas rich aqueous dispersion, transporting the gas rich aqueous dispersion to a second station and releasing the gas from the gas rich dispersion at the second station.
Claim 25
Dependent Claim
16 words
A method according to claim 15 wherein n=1, m=2 and each R is CH 3 .
Claim 26
Dependent Claim
49 words
A method according to claim 15 wherein one of B 1 and B 2 is a group of formula: --CO--(CH.sub.2).sub.8 --C═C--C═C--(CH.sub.2).sub.9 CH.sub.3 and the other of B 1 and B 2 is a mixture of --(CH 2 ) 15 CH 3 and --(CH 2 ) 17 CH 3 groups.
Claim 27
Dependent Claim
13 words
A method according to claim 15 wherein the ligand is deoxyhaemoglobin or haemoglobin.
References Cited (Backward Citations)
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