US5352435A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ionophore containing liposomes for ultrasound imaging
Assignee: Bristol Myers Squibb Pharma Co
•
Filing: 1992-10-27
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Granted: 1994-10-04
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Expires: 2012-10-27
Abstract
Abstract Liposomes suitable as ultrasound contrast agents which contain media of various types including gases, gaseous precursors activated by pH, temperature or pressure, as well as other solid or liquid contrast enhancing agents, are described. Methods of using the same as ultrasound contrast agents are also disclosed. The present invention also comprises novel methods for synthesizing liposomes having encapsulated therein gases.
Inventors
- 👤 Evan C. Unger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:02
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K41/00; A61K41/0028; A61K41/0052; A61K47/00; A61K47/50; A61K47/69; A61K47/6905; A61K47/6911; A61K47/6921; A61K47/6941; A61K49/00; A61K49/06; A61K49/18; A61K49/1806; A61K49/1815; A61K49/22; A61K49/222; A61K49/223; A61K49/227; A61K9/1277; A61K9/1278
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
18 words
A contrast agent for ultrasonic imaging which comprises an ionophore-containing liposome having encapsulated therein a pH-activated gaseous precursor.
Claim 2
Dependent Claim
18 words
A contrast agent for ultrasonic imaging which comprises an ionophore-containing liposome having encapsulated therein a pH-activated gaseous precursor.
Claim 3
Dependent Claim
25 words
A contrast agent according to claim 1 wherein said pH-activated gaseous precursor is selected from the group consisting of metal carbonate and metal bicarbonate salts.
Claim 4
Dependent Claim
15 words
A contrast agent according to claim 2 wherein said pH-activated gaseous precursor is sodium bicarbonate.
Claim 5
Dependent Claim
66 words
A contrast agent according to claim 1 wherein said ionophore in said ionophore-containing liposome is selected from the group consisting of carbonylcyanide, p-trifluoromethoxyphenylhydrazone, carbonylcyanide M-chlorophenylhydrazone, carbonylcyanide phenylhydrazine, tetrachloro-2-trifluoromethyl benzimidazole, 5,6-dichloro-2-trifluoromethyl benzimidazole, Uncoupler 1799, gramicidin, alamethicin, filipin, etruscomycin, nystatin, pimaricin, amphotericin, valinomycin, enniatin, beauvericin, monomycin, nonactin, monactin, dinactin, trinactin, tetranactin, antamanide, nigericin, monensin, salinomycin, narisin, mutalomycin, carriomycin, dianemycin, septamycin, A-204 A, X-206, X-537 A, A-23187 and dicyclohexyl-18-crown-6.
Claim 6
Dependent Claim
44 words
A contrast agent for ultrasonic imaging which comprises a liposome having encapsulated therein a solid contrast enhancing agent selected from the group consisting of magnetite and solid iodine particles or a liquid contrast enhancing agent selected from the group consisting of solubilized iodinated compounds.
Claim 7
Dependent Claim
44 words
A contrast agent for ultrasonic imaging which comprises a liposome having encapsulated therein a solid contrast enhancing agent selected from the group consisting of magnetite and solid iodine particles or a liquid contrast enhancing agent selected from the group consisting of solubilized iodinated compounds.
Claim 8
Dependent Claim
44 words
A contrast agent according to claim 1 wherein the liposome is comprised of lipid selected from the group consisting of fatty acids, lysolipids, dipalmitoylphosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, phosphatidylinositol, sphingomyelin, lysolipids, cholesterol, cholesterol hemisuccinate, glycosphingolipids, glycolipids, glucolipids, sulphatides, and tocopherol hemisuccinate.
Claim 9
Dependent Claim
14 words
A contrast agent according to claim 6 wherein the liposome is comprised of phosphatidylcholine.
Claim 10
Dependent Claim
16 words
A contrast agent according to claim 6 wherein the liposome is comprised of cholesterol and phosphatidylchcline.
Claim 11
Dependent Claim
24 words
A contrast agent according to claim 1 wherein the liposome has a mean outer diameter of between about 30 nanometers and about 2 microns.
Claim 12
Dependent Claim
24 words
A contrast agent according to claim 1 wherein the liposome has a mean outer diameter of between about 2 microns and about 10 microns.
Claim 13
Dependent Claim
44 words
A contrast agent according to claim 5 wherein the liposome is comprised of lipids selected from the group consisting of fatty acids, lysolipids, dipalmitoylphosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, phosphatidylinositol, sphingomyelin, lysolipids, cholesterol, cholesterol hemisuccinate, glycosphingolipids, glycolipids, glucolipids, sulphatides, and tocopherol hemisuccinate.
Claim 14
Dependent Claim
14 words
A contrast agent according to claim 11 wherein the liposome is comprised of phosphatidylcholine.
Claim 15
Dependent Claim
16 words
A contrast agent according to claim 11 wherein the liposome is comprised of cholesterol and phosphatidylchcline.
Claim 16
Dependent Claim
24 words
A contrast agent according to claim 5 wherein the liposome has a mean outer diameter of between about 30 nanometers and about two microns.
Claim 17
Dependent Claim
24 words
A contrast agent according to claim 5 wherein the liposome has a mean outer diameter of between about 2 microns and about 10 microns.
Claim 18
Dependent Claim
26 words
A contrast agent according to claim 5 wherein said contrast enhancing agent is a solid selected from the group consisting of magnetite and solid iodine particles.
Claim 19
Dependent Claim
16 words
A contrast agent according to claim 16 wherein said solid iodine particles are iodipamide ethyl ester.
Claim 20
Dependent Claim
24 words
A contrast agent according to claim 5 wherein said contrast enhancing agent is a liquid selected from the group consisting of solubilized iodinated compounds.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-03 | 1998-04-04 | 1998-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-03 | 2002-04-04 | 2002-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-03 | 2006-04-04 | 2006-10-04 | $7700.00 | Window Closed / Lapsed |