US5811447A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Therapeutic inhibitor of vascular smooth muscle cells
Assignee: UAB Research Foundation
•
Filing: 1995-05-25
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Granted: 1998-09-22
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Expires: 2015-05-25
Abstract
Abstract Methods are provided for inhibiting stenosis following vascular trauma or disease in a mammalian host, comprising administering to the host a therapeutically effective dosage of a therapeutic conjugate containing a vascular smooth muscle binding protein that associates in a specific manner with a cell surface of the vascular smooth muscle cell, coupled to a therapeutic agent dosage form that inhibits a cellular activity of the muscle cell. Methods are also provided for the direct and/or targeted delivery of therapeutic agents to vascular smooth muscle cells that cause a dilation and fixation of the vascular lumen by inhibiting smooth muscle cell contraction, thereby constituting a biological stent. Also discussed are mechanisms for in vivo vascular smooth muscle cell proliferation modulation, agents that impact those mechanisms and protocols for the use of those agents.
Inventors
- 👤 Lawrence L. Kunz
- 👤 Richard A. Klein
- 👤 John M. Reno
- 👤 David J. Grainger
- 👤 James C. Metcalfe
- 👤 Peter L. Weissberg
- 👤 Peter G. Anderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:37
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/0012; A61K9/0019; A61K9/0024; A61K31/00; A61K31/13; A61K31/135; A61K31/138; A61K31/33; A61K31/335; A61K31/337; A61K31/395; A61K31/40; A61K31/4025; A61K31/403; A61K31/4035; A61K31/407; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6801; A61K47/6803; A61K47/6807; A61K47/6809; A61K47/6811; A61K47/6817; A61K47/6831; A61K47/6835; A61K47/6843; A61K47/69; A61K47/6921; A61K47/6927; A61K47/6929; A61K47/6931; G; G01; G01N; G01N2800/00; G01N2800/32; G01N2800/323
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
61 words
A method for biologically stenting a mammalian blood vessel, which method comprises: administering to the blood vessel of a mammal cytochalasin B or a cytochalasin that is a functional analog of cytochalasin B in an amount and for a period of time effective to inhibit the contraction of vascular smooth muscle cells while not eliminating their ability to secrete extracellular matrix.
Claim 2
Independent Claim
61 words
A method for biologically stenting a mammalian blood vessel, which method comprises: administering to the blood vessel of a mammal cytochalasin B or a cytochalasin that is a functional analog of cytochalasin B in an amount and for a period of time effective to inhibit the contraction of vascular smooth muscle cells while not eliminating their ability to secrete extracellular matrix.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the vessel is subjected to angioplasty.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein the cytochalasin is infused to achieve a concentration of about 10 -3 to 10 -12 M.
Claim 5
Dependent Claim
16 words
The method of claim 1 wherein the cytochalasin is locally administered in one or more doses.
Claim 6
Dependent Claim
12 words
The method of claim 4 wherein the cytochalasin is administered by catheter.
Claim 7
Dependent Claim
12 words
The method of claim 1 wherein the cytochalasin is administered in solution.
Claim 8
Dependent Claim
19 words
The method of claim 1 further comprising the administration of a cytostatic agent in a sustained release dosage form.
Claim 9
Dependent Claim
18 words
The method of claim 7 wherein the cytostatic agent is effective to inhibit cellular proliferation subsequent to stenting.
Claim 10
Dependent Claim
34 words
A therapeutic method comprising maintaining or expanding blood vessel luminal area by systemically administering to a mammal an effective amount of cytochalasin B or a cytochalasin that is a functional analog of cytochalasin B.
Claim 11
Dependent Claim
34 words
A therapeutic method comprising maintaining or expanding blood vessel luminal area by systemically administering to a mammal an effective amount of cytochalasin B or a cytochalasin that is a functional analog of cytochalasin B.
Claim 12
Dependent Claim
21 words
The method of claim 9 wherein the cytostatic agent comprises cytochalasin B or a cytochalasin that is a functional analog thereof.
Claim 13
Dependent Claim
23 words
The method of claim 10 wherein the sustained release formulation comprises microparticles or nanoparticles comprising said cytochalasin B or said functional analog thereof.
Claim 14
Independent Claim
45 words
A method for biologically stenting a mammalian blood vessel, which method comprises administering to the blood vessel of a mammal a cytoskeletal inhibitor in an amount and for a period of time effective to inhibit the contraction or migration of the vascular smooth muscle cells.
Claim 15
Independent Claim
45 words
A method for biologically stenting a mammalian blood vessel, which method comprises administering to the blood vessel of a mammal a cytoskeletal inhibitor in an amount and for a period of time effective to inhibit the contraction or migration of the vascular smooth muscle cells.
Claim 16
Dependent Claim
16 words
The method of claim 10 wherein the cytostatic agent comprises taxol or a structural analog thereof.
Claim 17
Dependent Claim
23 words
The method of claim 10 wherein the sustained release formulation comprises microparticles or nanoparticles comprising said cytochalasin B or said functional analog thereof.
Claim 18
Dependent Claim
23 words
The method of claim 1 wherein the administration of cytochalasin B or a functional analog thereof is before, during or after the trauma.
Claim 19
Dependent Claim
23 words
The method of claim 10 wherein the administration of cytochalasin B or a functional analog thereof is before, during or after the trauma.
Claim 20
Dependent Claim
25 words
The method of claim 16 wherein the administration of cytochalasin B or a functional analog thereof and the administration of the cytostatic agent is simultaneous.
Claim 21
Dependent Claim
18 words
The method of claim 10 wherein the administration of the cytostatic agent is before or after the trauma.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-09-21 | 2002-03-23 | 2002-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-09-21 | 2006-03-23 | 2006-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-09-21 | 2010-03-23 | 2010-09-22 | $7700.00 | Window Closed / Lapsed |