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US5185260A Expired / Lapsed US ⏱️ 0.0 years remaining

Method for distinguishing normal and transformed cells using G1 kinase inhibitors

Assignee: United States Department of Energy
•
Filing: 1991-08-29
•
Granted: 1993-02-09
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Expires: 2011-08-29
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Abstract

Abstract A G1 phase kinase inhibitor is applied in a low concentration to a population of normal and transformed mammalian cells. The concentration of G1 phase kinase inhibitor is selected to reversibly arrest normal mammalian cells in the G1 cell cycle without arresting growth of transformed cells. The transformed cells may then be selectively identified and/or cloned for research or diagnostic purposes. The transformed cells may also be selectively killed by therapeutic agents that do not affect normal cells in the G1 phase, suggesting that such G1 phase kinase inhibitors may form an effective adjuvant for use with chemotherapeutic agents in cancer therapy for optimizing the killing dose of chemotherapeutic agents while minimizing undesirable side effects on normal cells.

Inventors

  • 👤 Harry A. Crissman
  • 👤 Donna M. Gadbois
  • 👤 Robert A. Tobey
  • 👤 E. Morton Bradbury

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 03:30
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/33; A61K31/395; A61K31/55; A61K31/40; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5306

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 43 words
A method for distinguishing between normal and transformed mammalian cells, comprising the step of; subjecting said normal and transformed cells to low concentrations of a G 1 kinase inhibitor effective to arrest said normal cells in G 1 without arresting said transformed cells.
Claim 2
Independent Claim 43 words
A method for distinguishing between normal and transformed mammalian cells, comprising the step of; subjecting said normal and transformed cells to low concentrations of a G 1 kinase inhibitor effective to arrest said normal cells in G 1 without arresting said transformed cells.
Claim 3
Dependent Claim 12 words
A method according to claim 1, wherein said kinase inhibitor is staurosporine.
Claim 4
Dependent Claim 17 words
A method according to claim 2, wherein said low concentrations are in the range of 1-10 ng/ml.
Claim 5
Dependent Claim 50 words
A method according to claim 1, wherein said kinase inhibitor is selected from the group consisting of ______________________________________ ##STR3## and ##STR4## K252b KT5720 KT5823 KT5926 ______________________________________ R1 H H CH.sub.3 H R2 H n-Hex CH.sub.3 CH.sub.3 R3 H H CH.sub.3 H R4 H H H OCH.sub.2 CH.sub.2 CH.sub. ______________________________________ 3.
Claim 6
Dependent Claim 44 words
A method according to claim 1, further including the steps of: growing cultures of said normal and said transformed cells in the presence of said G 1 kinase inhibitor; and determining the relative growth of cells in said cultures to identify said transformed cells.
Claim 7
Dependent Claim 44 words
A method according to claim 2, further including the steps of: growing cultures of said normal and said transformed cells in the presence of said G 1 kinase inhibitor; and determining the relative growth of cells in said cultures to identify said transformed cells.
Claim 8
Dependent Claim 44 words
A method according to claim 4, further including the steps of: growing cultures of said normal and said transformed cells in the presence of said G 1 kinase inhibitor; and determining the relative growth of cells in said cultures to identify said transformed cells.

References Cited (Backward Citations)

US4877776A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1996-02-09 1996-08-10 1997-02-09 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2000-02-09 2000-08-10 2001-02-09 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2004-02-09 2004-08-10 2005-02-09 $7700.00 Window Closed / Lapsed

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