US7122356B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for attachment of biomolecules to medical device surfaces
Assignee: Medtronic Inc
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Filing: 2003-07-15
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Granted: 2006-10-17
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Expires: 2023-07-15
Abstract
Abstract A method for making a medical device having at least one biomolecule immobilized on a substrate surface is provided. One method of the present invention includes immobilizing a biomolecule comprising an unsubstituted amide moiety on a biomaterial surface. Another method of the present invention includes immobilizing a biomolecule on a biomaterial surface comprising an unsubstituted amide moiety. Still another method of the present invention may be employed to crosslink biomolecules comprising unsubstituted amide moieties immobilized on medical device surfaces. Additionally, one method of the present invention may be employed to crosslink biomolecules comprising unsubstituted amide moieties in solution, thereby forming a crosslinked biomaterial or a crosslinked medical device coating.
Inventors
- 👤 James R. Keogh
- 👤 Paul V. Trescony
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:28
CPC Classifications
A; A61; A61L; A61L33/00; A61L33/18; A61L27/00; A61L27/14; A61L27/22; A61L27/24; A61L27/28; A61L27/34; A61L29/00; A61L29/08; A61L29/085; A61L31/00; A61L31/08; A61L31/10; A61L33/0005; A61L33/0011; A61L33/0029; A61L33/0047; A61L33/0076; A61L33/0082; A61L33/06; A61L33/12; A61L33/128; C; C08; C08L; C08L89/00; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
149 words
A method of forming a crosslinked coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the method comprising the steps of: (a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising unsubstituted amide moieties; (b) combining the amide moieties with an amine forming agent to form amine moieties; (c) providing two or more biomolecules, the biomolecules comprising an amine moiety and a 1,2-dihydroxy moiety, combining a periodate with the biomolecules to oxidize the 1,2-dihydroxy moieties to form aldehyde moieties; (d) combining the biomolecules with the surface; (e) allowing the aldehyde moieties to combine with the amine moieties to form imine moieties; and (f) reacting the imine moieties with a reducing agent to form amine linkages, the amine linkages immobilizing and crosslinking the biomolecules on the surface, the immobilized and crosslinked biomolecules forming the coating.
Claim 2
Independent Claim
149 words
A method of forming a crosslinked coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the method comprising the steps of: (a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising unsubstituted amide moieties; (b) combining the amide moieties with an amine forming agent to form amine moieties; (c) providing two or more biomolecules, the biomolecules comprising an amine moiety and a 1,2-dihydroxy moiety, combining a periodate with the biomolecules to oxidize the 1,2-dihydroxy moieties to form aldehyde moieties; (d) combining the biomolecules with the surface; (e) allowing the aldehyde moieties to combine with the amine moieties to form imine moieties; and (f) reacting the imine moieties with a reducing agent to form amine linkages, the amine linkages immobilizing and crosslinking the biomolecules on the surface, the immobilized and crosslinked biomolecules forming the coating.
Claim 3
Dependent Claim
24 words
The method of claim 1 , wherein the periodate comprises at least one of periodic acid, sodium periodate, alkali metal periodates, and potassium periodate.
Claim 4
Dependent Claim
28 words
The method of claim 1 , wherein the periodate is combined with the 2-aminoalcohol moieties in an aqueous solution having a pH between about 4 and about 9.
Claim 5
Dependent Claim
30 words
The method of claim 1 , wherein the periodate is combined with the 2-aminoalcohol moieties in an aqueous solution having a temperature between about 0 and about 50 degrees Celsius.
Claim 6
Dependent Claim
17 words
The method of claim 1 , wherein the oxidizing step is performed in the absence of light.
Claim 7
Dependent Claim
27 words
The method of claim 1 , wherein the biomolecules and the surface are combined in an aqueous solution having a pH between about 6 and about 10.
Claim 8
Dependent Claim
29 words
The method of claim 1 , wherein the biomolecules and the surface are combined in an aqueous solution having a temperature between about 0 and about 50 degrees Celsius.
Claim 9
Dependent Claim
22 words
The method of claim 1 , wherein the reducing agent comprises at least one of sodium borohydride, sodium cyanoborohydride, and amine borane.
Claim 10
Dependent Claim
29 words
The method of claim 1 , wherein the reducing agent is combined with the imine moieties in an aqueous solution having a pH between about 6 and about 10.
Claim 11
Dependent Claim
31 words
The method of claim 1 , wherein the reducing agent is combined with the imine moieties in an aqueous solution having a temperature between about 0 and about 50 degrees Celsius.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2009-10-16 | 2010-04-17 | 2010-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2013-10-16 | 2014-04-17 | 2014-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2017-10-16 | 2018-04-17 | 2018-10-17 | $7700.00 | Window Closed / Lapsed |