US5820581A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for cleaning large bone grafts and bone grafts produced thereby
Assignee: Lifenet Health
•
Filing: 1996-03-21
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Granted: 1998-10-13
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Expires: 2016-03-21
Abstract
Abstract A process for removing substantially all bone marrow from a large bone and a bone graft produced thereby. A large substantially intact bone is selected and excess cartilage is removed from at least one articulating surface of the bone. An opening is prepared through the cortical layer of the bone to permit access of a vacuum line to the bone cavity. A vacuum line is attached to the bone via the opening for application of vacuum to the bone cavity, and the opening is sealed. A vacuum is applied to draw a first cleaning solution through the bone cavity so as to draw the first solution and solubilized bone marrow through the vacuum line to exit the bone at the opening. The vacuum is discontinued when the bone has been substantially cleaned of bone marrow. Subsequently, a second flushing solution may be drawn through the bone cavity via vacuum.
Inventors
- 👤 Lloyd Wolfinbarger, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:49
CPC Classifications
A; A61; A61F; A61F2/00; A61F2/02; A61F2/30; A61F2/46; A61F2/4644; A61B; A61B17/00; A61B17/34; A61B17/3472; A61L; A61L2/00; A61L2/02; A61L2/025; A61L2/16; A61L2/18; C; C11; C11D; C11D1/00; C11D1/66; C11D1/83; A61B10/00; A61B10/02; A61B10/0233; A61B10/025; A61B2010/0258; A61B2017/00969; A61B90/00; A61B90/70; A61F2/28; A61F2002/2835; A61F2002/286; A61F2002/2864; A61F2002/4646; A61F2002/4649; A61F2002/4681; A61F2002/4683; A61F2002/4685; A61L2103/00; A61L2103/05; A61L2430/00; A61L2430/02; A61N; A61N7/00; C11D1/02; C11D1/12; C11D1/29; C11D1/72; C11D2111/00; C11D2111/40; C11D2111/46
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
38 words
A process for cleaning an essentially intact bone graft, comprising: applying a vacuum to an essentially intact bone to draw solution comprising at least one solvent for bone marrow through said essentially intact bone to solubilize bone marrow.
Claim 2
Dependent Claim
38 words
A process for cleaning an essentially intact bone graft, comprising: applying a vacuum to an essentially intact bone to draw solution comprising at least one solvent for bone marrow through said essentially intact bone to solubilize bone marrow.
Claim 3
Dependent Claim
41 words
The process according to claim 1, wherein the solution comprises about 0.0001× to 10× of a 1× detergent solution containing about 0.066 weight percent Brij-35, about 0.02 weight percent Nonidet P-40, and about 0.02 weight percent Nonoxynol-9 in endotoxin free water.
Claim 4
Dependent Claim
19 words
The process according to claim 2, wherein the solution comprises about 0.001× to 0.01× of the 1× detergent solution.
Claim 5
Dependent Claim
19 words
The process according to claim 3, wherein the solution comprises about 0.001× to 0.01× of the 1× detergent solution.
Claim 6
Dependent Claim
18 words
The process according to claim 4, wherein the solution comprises about 0.005× to 0.01×°of the 1× detergent solution.
Claim 7
Dependent Claim
39 words
The process according to claim 1, wherein the solution comprises endotoxin-free deionized/distilled water and at least one detergent selected from the group consisting of anionic detergents and nonanionic detergents, in concentrations ranging from about 0.001 to 2 weight percent.
Claim 8
Dependent Claim
12 words
The process according to claim 6, wherein the solution further comprises ethanol.
Claim 9
Dependent Claim
34 words
A process for cleaning a essentially intact bone graft, comprising: subjecting bone marrow of said essentially intact bone graft to a negative pressure-mediated flow of solvent under conditions effective to solubilize said bone marrow.
Claim 10
Dependent Claim
34 words
A process for cleaning a essentially intact bone graft, comprising: subjecting bone marrow of said essentially intact bone graft to a negative pressure-mediated flow of solvent under conditions effective to solubilize said bone marrow.
Claim 11
Dependent Claim
27 words
The process of claim 8, wherein said solubilized essentially intact bone marrow exits said essentially intact bone through an opening in a cortical layer of said bone.
Claim 12
Dependent Claim
18 words
The process of any one of claims 8 or 9, wherein said solvent comprises one or more detergents.
Claim 13
Dependent Claim
39 words
A process for cleaning an essentially intact bone graft, comprising: Subjecting bone marrow of said essentially intact bone graft to negative pressure under conditions effective to remove substantially all of said bone marrow from said essentially intact bone graft.
Claim 14
Dependent Claim
39 words
A process for cleaning an essentially intact bone graft, comprising: Subjecting bone marrow of said essentially intact bone graft to negative pressure under conditions effective to remove substantially all of said bone marrow from said essentially intact bone graft.
Claim 15
Dependent Claim
25 words
The process of any one of claims 1, 8, or 11, wherein said essentially intact bone graft is at least one-third of an intact bone.
Claim 16
Dependent Claim
19 words
The process of claim 12, wherein said essentially intact bone graft is at least one-half of an intact bone.
Claim 17
Dependent Claim
27 words
The process of claim 13, wherein said bone is selected from the group consisting of: a proximal femur; a femur; a ilia; a humerus; and a tibia.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-10-12 | 2002-04-13 | 2002-10-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-10-12 | 2006-04-13 | 2006-10-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-10-12 | 2010-04-13 | 2010-10-13 | $7700.00 | Window Closed / Lapsed |