US5118512A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for cryopreserving biological materials and materials prepared thereby
Assignee: Warsaw Orthopedic Inc
•
Filing: 1990-01-23
•
Granted: 1992-06-02
•
Expires: 2010-01-23
Abstract
Abstract A process for cryopreserving a biological material which comprises contacting the biological material with both an effective amount at least one cryopreservation agent, and an agent to increase diffusion of the cryopreservation agent into the biological material, such as a surfactant or a permeation enhancer.
Inventors
- 👤 Robert K. O'Leary
- 👤 Ann Prewett
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:43
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
40 words
A process for cryopreserving bone, comprising: contacting bone with both an effective amount of at least one cryopreservation agent, and an agent to increase diffusion of the at least one cryopreservation agent into the bone and then freezing said bone.
Claim 2
Dependent Claim
40 words
A process for cryopreserving bone, comprising: contacting bone with both an effective amount of at least one cryopreservation agent, and an agent to increase diffusion of the at least one cryopreservation agent into the bone and then freezing said bone.
Claim 3
Dependent Claim
41 words
The process of claim 1 wherein the cryopreservation agent is selected from the class consisting of dimethyl sulfoxide, glycerol, liquid carbon dioxide, fish blood glycoproteins isolated from species of the genus Trematomus or Borchgrebinki arachis, formamide, polyvinyl pyrrolidone, and hydroxyethyl starch.
Claim 4
Dependent Claim
21 words
The process of claim 1 wherein said agent to increase diffusion of the cryopreservation agent into the bone is a surfactant.
Claim 5
Dependent Claim
12 words
The process of claim 3 wherein the surfactant is a nonionic surfactant.
Claim 6
Dependent Claim
22 words
The process of claim 1 wherein said agent to increase diffusion of the cryopreservation agent into the bone is a permeation enhancer.
Claim 7
Dependent Claim
17 words
The process of claim 3 wherein the surfactant is miscible with said at least one cryopreservation agent.
Claim 8
Dependent Claim
31 words
The process of claim 1 wherein said at least one cryopreservation agent and said agent to increase diffusion of the cryopreservation agent into the bone are applied as an aqueous mixture.
Claim 9
Dependent Claim
28 words
The process of claim 1 wherein said at least one cryopreservation agent is added in an aqueous solution in an amount of from about 1% to about 25%.
Claim 10
Dependent Claim
34 words
The process of claim 1 wherein the agent to increase diffusion of the cryopreservation agent into the bone is added in an aqueous solution in an amount of from about 0.0001% to about 10%.
Claim 11
Dependent Claim
12 words
A product comprising a bone prepared by the process of claim 1.
Claim 12
Dependent Claim
14 words
The process of claim 4 wherein said non-ionic surfactant is an octylphenoxy polyethoxyethanol surfactant.
References Cited (Backward Citations)
US4559298A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-06-02 | 1995-12-02 | 1996-06-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-06-02 | 1999-12-02 | 2000-06-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-06-02 | 2003-12-02 | 2004-06-02 | $7700.00 | Window Closed / Lapsed |