US5558875A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of preparing collagenous tissue
Assignee: Individual
•
Filing: 1994-06-06
•
Granted: 1996-09-24
•
Expires: 2014-06-06
Abstract
Abstract A process of preparation of a collagenous prosthesis for removing cell membrane proteins from a biological collagenous tissue and preserving elasticity of the collagenous prosthesis comprises the following steps: (a) soaking the tissue in an organic detergent for a sufficient time to disrupt the cell membrane and to solubilize the cellular membrane proteins of the collagenous tissue; (b) extracting and removing the cellular membrane proteins from the collagenous tissue by mechanical washing to obtain the collagenous prosthesis; (c) preserving the collagenous prosthesis in alcohol.
Inventors
- 👤 Su Wang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:47
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3691; A61L27/14; A61L27/22; A61L27/24; A61L27/3687; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; A61L2430/00; A61L2430/40; Y; Y10; Y10S; Y10S623/00; Y10S623/901
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
88 words
A process of preparing collagenous prosthesis having elasticity from a biological collagenous tissue consisting of the following steps in order: (a) soaking the tissue in an organic detergent for a sufficient time to disrupt cell membranes and to solubilize cellular membrane proteins of the collagenous tissue; (b) washing the tissue with the organic detergent to remove the solubilized cellular membrane proteins; (c) rinsing the tissue with distilled water to obtain the collagenous prosthesis having elasticity; (d) storing the prosthesis in alcohol as a means to preserve the elasticity.
Claim 2
Dependent Claim
88 words
A process of preparing collagenous prosthesis having elasticity from a biological collagenous tissue consisting of the following steps in order: (a) soaking the tissue in an organic detergent for a sufficient time to disrupt cell membranes and to solubilize cellular membrane proteins of the collagenous tissue; (b) washing the tissue with the organic detergent to remove the solubilized cellular membrane proteins; (c) rinsing the tissue with distilled water to obtain the collagenous prosthesis having elasticity; (d) storing the prosthesis in alcohol as a means to preserve the elasticity.
Claim 3
Dependent Claim
26 words
The process according to claim 1, wherein said organic detergent is an amphipathic molecule having both hydrophobic and hydrophilic regions which bind to lipids and proteins.
Claim 4
Dependent Claim
27 words
The process according to claim 1, wherein said tissue of step (a) is soaked in the detergent for 2 to 20 days to disrupt the cell membranes.
Claim 5
Dependent Claim
30 words
The process according to claim 1 wherein said tissue is selected from the group consisting of blood vessels, venous valves, heart valves, skin, tendon, bone, pericardium, cornea, and umbilical cord.
Claim 6
Dependent Claim
17 words
The process according to claim 4, wherein said blood vessel has a diameter less than 3 mm.
Claim 7
Dependent Claim
28 words
The process according to claim 1, wherein said washing of step (b) is mechanical shaking and stirring of the tissue and applying pulsatile pressure to the collagenous tissue.
Claim 8
Dependent Claim
16 words
The process according to claim 1, wherein said-organic detergent of step (a) is sodium dodecyl sulfate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-09-24 | 2000-03-25 | 2000-09-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-09-24 | 2004-03-25 | 2004-09-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-09-24 | 2008-03-25 | 2008-09-24 | $7700.00 | Window Closed / Lapsed |