US6608040B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Chemical modification of biomedical materials with genipin
Assignee: Challenge Bioproducts Co Ltd
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Filing: 2001-09-27
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Granted: 2003-08-19
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Expires: 2021-09-27
Abstract
Abstract Biocompatible cross-linked materials, suitable for use in implants, wound dressings, and blood substitutes, are described. The materials are prepared by cross-linking biological substances, such as collagen, chitosan, or hemoglobin, with genipin, a naturally occurring cross-linking agent. The cross-linking agent has much lower toxicity than conventionally used reagents, and the cross-linked products have good thermal and mechanical stability as well as biocompatibility.
Inventors
- 👤 Ching-Kuan Lin
- 👤 Thomas Chau-Jen Lee
- 👤 Hsing-Wen Sung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 02:43
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/50; A61L27/507; A61L15/00; A61L15/16; A61L15/22; A61L15/28; A61L15/32; A61L15/325; A61L24/00; A61L24/04; A61L24/10; A61L24/102; A61L24/104; A61L26/00; A61L26/0009; A61L26/0023; A61L26/0028; A61L26/0033; A61L26/0038; A61L27/14; A61L27/20; A61L27/22; A61L27/222; A61L27/24; A61L27/36; A61L27/3604; A61L27/3616; A61L27/3641; A61L27/3645; A61L27/3662; A61L27/3683; A61L27/3687; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; A61F2310/00383; A61L2430/00; A61L2430/40
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
57 words
A method of providing a biocompatible implant, comprising (i) procuring a tissue material comprising genipin-crosslinkable collagenous tissue, wherein the tissue material is characterized by suitable tensile strength, and (ii) crosslinking said tissue material with genipin to produce a crosslinked structure, said crosslinked structure having stability to degradative enzymes, low acute toxicity, and durability as the biocompatible implant.
Claim 2
Independent Claim
57 words
A method of providing a biocompatible implant, comprising (i) procuring a tissue material comprising genipin-crosslinkable collagenous tissue, wherein the tissue material is characterized by suitable tensile strength, and (ii) crosslinking said tissue material with genipin to produce a crosslinked structure, said crosslinked structure having stability to degradative enzymes, low acute toxicity, and durability as the biocompatible implant.
Claim 3
Dependent Claim
16 words
The method of claim 1 , wherein said genipin-crosslinkable collagenous tissue comprises an amine containing molecule.
Claim 4
Dependent Claim
20 words
The method of claim 1 , wherein said tissue material comprises gelatin, the gelatin being derived from a collagen source.
Claim 5
Dependent Claim
15 words
The method of claim 1 , wherein said crosslinked structure comprises a prosthetic heart valve.
Claim 6
Dependent Claim
14 words
The method of claim 1 , wherein said crosslinked structure comprises a ligament replacement.
Claim 7
Dependent Claim
14 words
The method of claim 1 , wherein said crosslinked structure comprises a vascular graft.
Claim 8
Independent Claim
38 words
A method of preparing a biocompatible adhesive comprising crosslinking a gelatin with an amount of genipin effective to yield a desired adhesiveness and consistency, wherein said gelatin is derived from hydrolytic treatment of collagen or a collagen-containing tissue.
Claim 9
Independent Claim
38 words
A method of preparing a biocompatible adhesive comprising crosslinking a gelatin with an amount of genipin effective to yield a desired adhesiveness and consistency, wherein said gelatin is derived from hydrolytic treatment of collagen or a collagen-containing tissue.
Claim 10
Dependent Claim
14 words
A composition suitable for use in a blood substitute, comprising hemoglobin crosslinked with genipin.
Claim 11
Dependent Claim
14 words
A composition suitable for use in a blood substitute, comprising hemoglobin crosslinked with genipin.
Claim 12
Dependent Claim
24 words
The composition of claim 8 , wherein the crosslinked hemoglobin has a mean degree of polymerization greater than 1 and less than about 9.
Claim 13
Independent Claim
19 words
A method of preparing crosslinked hemoglobin, comprising treating hemoglobin with an amount of genipin effective to crosslink said hemoglobin.
Claim 14
Independent Claim
19 words
A method of preparing crosslinked hemoglobin, comprising treating hemoglobin with an amount of genipin effective to crosslink said hemoglobin.
Claim 15
Dependent Claim
32 words
The method of claim 10 , wherein the hemoglobin is treated with an amount of genipin effective to give a mean degree of polymerization greater than 1 and less than about 9.
Claim 16
Dependent Claim
50 words
The method of claim 10 , wherein said hemoglobin is contacted with said genipin in an aqueous medium having a neutral or basic pH, in a molar ratio of about 6:1 to 9:1, for a period of about 12-15 hours at a temperature of about 4° C. to 20° C.
Claim 17
Dependent Claim
12 words
The crosslinked hemoglobin as prepared by the method of claim 12 .
Claim 18
Dependent Claim
14 words
The method of claim 1 , wherein said crosslinked structure comprises a pericardial patch.
Claim 19
Independent Claim
67 words
A method of providing a biocompatible wound dressing comprising: (i) procuring a tissue material comprising genipin-crosslinkable collagenous tissue; (ii) crosslinking said tissue material with genipin to produce a crosslinked structure, said crosslinked structure having stability to degradative enzymes and low acute toxicity as the biocompatible wound dressing; and (iii) fabricating said crosslinked structure to a proper shape and size configured for use as the biocompatible wound dressing.
Claim 20
Independent Claim
67 words
A method of providing a biocompatible wound dressing comprising: (i) procuring a tissue material comprising genipin-crosslinkable collagenous tissue; (ii) crosslinking said tissue material with genipin to produce a crosslinked structure, said crosslinked structure having stability to degradative enzymes and low acute toxicity as the biocompatible wound dressing; and (iii) fabricating said crosslinked structure to a proper shape and size configured for use as the biocompatible wound dressing.
Claim 21
Dependent Claim
16 words
The method of claim 15 , wherein said genipin-crosslinkable collagenous tissue comprises an amine containing molecule.
Claim 22
Dependent Claim
12 words
The method of claim 15 , wherein said tissue material comprises chitosan.
References Cited (Backward Citations)
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