US5521154A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Imidoester cross-linked hemoglobin compositions
Assignee: Pharmacia and Upjohn Co
•
Filing: 1994-06-15
•
Granted: 1996-05-28
•
Expires: 2014-06-15
Abstract
Abstract An imidoester, cross-linked hemoglobin composition useful in the transport of oxygen to living cells and being essentially free of any impurities, a P50 of at least 13mm Hg and predominantly in tetraruer form. Preferably, the cross-linked hemoglobin composition has a predominant molecular weight of at least 64,000. The purified and cross-linked hemoglobin has improved cross-link stability to autoxidation and can be used as a blood substitute for mammals or as an oxygen transport fluid.
Inventors
- 👤 Robert L. Garlick
- 👤 Joseph P. Martin, Jr.
- 👤 Stephen B. Lyle
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:39
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/795; C07K14/805; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
81 words
A cross-linked hemoglobin for transporting oxygen in living cells having a P50 of at least 13 and wherein at least 80% of said hemoglobin has a molecular weight of at least 64,000 made by the process comprising: cross-linking a deoxygenated hemoglobin lysate with dimethyl adipimidate or dimethyl suberimidate in a polymerization solution having a pH of at least 8 and comprising 50 nM Tris-HCl, NaCl and a buffer selected from the group consisting of 2-amino-2-methyl-1-propanol, Tris, sodium carbonate, CHES and CAPSO.
Claim 2
Dependent Claim
81 words
A cross-linked hemoglobin for transporting oxygen in living cells having a P50 of at least 13 and wherein at least 80% of said hemoglobin has a molecular weight of at least 64,000 made by the process comprising: cross-linking a deoxygenated hemoglobin lysate with dimethyl adipimidate or dimethyl suberimidate in a polymerization solution having a pH of at least 8 and comprising 50 nM Tris-HCl, NaCl and a buffer selected from the group consisting of 2-amino-2-methyl-1-propanol, Tris, sodium carbonate, CHES and CAPSO.
References Cited (Backward Citations)
US3925344A
Cited
US4001401A
Cited
US4061736A
Cited
US4001200A
Cited
US4053590A
Cited
US4777244A
Cited
EP0181033A1
Cited
EP0195558A2
Cited
US4826811A
Cited
WO1988003408A1
Cited
US4857636A
Cited
EP0361720A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-05-28 | 1999-11-27 | 2000-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-05-28 | 2003-11-27 | 2004-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-05-28 | 2007-11-27 | 2008-05-28 | $7700.00 | Window Closed / Lapsed |