US5928916A
Expired / Lapsed
US
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Ionic attachment of biomolecules with a guanidino moiety to medical device surfaces
Assignee: Medtronic Inc
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Filing: 1998-01-22
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Granted: 1999-07-27
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Expires: 2018-01-22
Abstract
Abstract Methods are provided for forming a coating of an immobilized biomolecule on a surface of a medical device to impart improved biocompatibility for contacting tissue and bodily fluids. A biomolecule having a negatively charged moiety is combined with a medical device surface having a positively charged guanidino moiety to form an ionic bond immobilizing a coating of the biomolecule on the surface. In another method, the medical device surface contains an amine moiety that is combined with a guanidino forming agent to form a positively charged guanidino moiety that is combined with the negatively charged moiety to form the ionic bond. In a further embodiment, the medical device surface contains a negatively charged moiety, and a biomolecule containing an amine moiety is combined with a guanidino forming agent to form a positively charged guanidino moiety that is combined with the negatively charged moiety to form the ionic bond.
Inventors
- 👤 James R. Keogh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 22:57
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/28; A61K; A61K38/00; A61K38/16; A61K38/43; A61L27/14; A61L27/22; A61L27/24; A61L27/34; A61L27/36; A61L27/3604; A61L27/3625; A61L27/3641; A61L27/3645; A61L27/50; A61L27/54; A61L29/00; A61L29/08; A61L29/085; A61L31/00; A61L31/08; A61L31/10; A61L31/14; A61L31/16; A61L33/00; A61L33/0005; A61L33/0011; A61L33/0029; A61L33/0047; A61L33/0076; A61L33/0082; A61L33/06; A61L33/12; A61L33/128; A61L33/18; C; C08; C08L; C08L89/00; C12; C12N; C12N11/00; C12N11/02; C12N11/14; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; A61L2300/00; A61L2300/60; A61L2300/606; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
111 words
A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissue or fluids in or temporarily removed from a living mammalian subject, the method comprising: (a) providing the medical device, the medical device having a suitable biomaterial forming the surface, the biomaterial comprising a positively charged guanidino moiety; (b) providing a biomolecule, the biomolecule comprising a negatively charged moiety; and (c) combining the negatively charged moiety with the positively charged guanidino moiety to form an ionic bond, the ionic bond immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.
Claim 2
Independent Claim
111 words
A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissue or fluids in or temporarily removed from a living mammalian subject, the method comprising: (a) providing the medical device, the medical device having a suitable biomaterial forming the surface, the biomaterial comprising a positively charged guanidino moiety; (b) providing a biomolecule, the biomolecule comprising a negatively charged moiety; and (c) combining the negatively charged moiety with the positively charged guanidino moiety to form an ionic bond, the ionic bond immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.
Claim 3
Dependent Claim
72 words
The method of claim 1, wherein the medical device is selected from the group consisting of a blood-contacting medical device, a tissue-contacting medical device, a bodily fluid-contacting medical device, an implantable medical device, an extracorporeal medical device, a blood oxygenator, a blood pump, tubing for carrying blood, an endoprosthesis medical device, a vascular graft, a stent, a pacemaker lead, a heart valve, temporary intravascular medical device, a catheter and a guide wire.
Claim 4
Dependent Claim
24 words
The method of claim 1, wherein the negatively charged moiety is selected from the group consisting of a phosphate, a sulphate and a carboxylate.
Claim 5
Dependent Claim
14 words
The method of claim 1, wherein the guanidino moiety is an arginine amino acid.
Claim 6
Dependent Claim
13 words
The method of claim 1, wherein the biomolecule is a naturally occurring biomolecule.
Claim 7
Dependent Claim
13 words
The method of claim 1, wherein the biomolecule is a chemically synthesized biomolecule.
Claim 8
Dependent Claim
90 words
The method of claim 1, wherein the biomolecule is selected from the group consisting of a globular protein, a cell attachment protein, a protein, a cell attachment peptide, a peptide, an anti-inflammatory agent, an antibody, an antigen, an immunoglobulin, an enzyme, a hormone, a growth factor, a neurotransmitter, a cytokine, a proteoglycan, a toxin, an antibiotic agent, an antibacterial agent, an antimicrobial agent, an antithrombotic agent, a polysaccharide, a carbohydrate, a fatty acid, a nucleic acid, a DNA segment, RNA segment, a lectin, a drug, a vitamin and a ligand.
Claim 9
Dependent Claim
31 words
The method of claim 1, wherein at least a portion of the surface forms at least one of a tube, a rod, a membrane, a balloon, a bag and a sheet.
Claim 10
Dependent Claim
93 words
The method of claim 1, wherein the medical device comprises at least one of a biocompatible material selected from the group consisting of metal, titanium, titanium alloys, tin-nickel alloys, shape memory alloys, aluminum oxide, platinum, platinum alloys, stainless steel, MP35N stainless steel, elgiloy, stellite, pyrolytic carbon, silver carbon, glassy carbon, polymer, polyamide, polycarbonate, polyether, polyester, polyolefin, polyethylene, polypropylene, polystyrene, polyurethane, polyvinyl chloride, polyvinylpyrrolidone, silicone elastomer, fluoropolymer, polyacrylate, polyisoprene, polytetrafluoroethylene, rubber, ceramic, hydroxapatite, human protein, human tissue, animal protein, animal tissue, bone, skin, teeth, collagen, laminin, elastin, fibrin, wood, cellulose, compressed carbon and glass.
Claim 11
Independent Claim
128 words
A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissue or fluids in or temporarily removed from a living mammalian subject, the method comprising: (a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising an amine moiety; (b) combining the amine moiety with a guanidino forming agent to form a positively charged guanidino moiety on the surface; (c) providing a biomolecule, the biomolecule comprising a negatively charged moiety; and (d) combining the negatively charged moiety with the positively charged guanidino moiety to form an ionic bond, the ionic bond immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.
Claim 12
Independent Claim
128 words
A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissue or fluids in or temporarily removed from a living mammalian subject, the method comprising: (a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising an amine moiety; (b) combining the amine moiety with a guanidino forming agent to form a positively charged guanidino moiety on the surface; (c) providing a biomolecule, the biomolecule comprising a negatively charged moiety; and (d) combining the negatively charged moiety with the positively charged guanidino moiety to form an ionic bond, the ionic bond immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.
Claim 13
Dependent Claim
71 words
The method of claim 10, wherein the device is selected from the group consisting of a blood-contacting medical device, a tissue-contacting medical device, a bodily fluid-contacting medical device, an implantable medical device, an extracorporeal medical device, a blood oxygenator, a blood pump, tubing for carrying blood, an endoprosthesis medical device, a vascular graft, a stent, a pacemaker lead, a heart valve, temporary intravascular medical device, a catheter and a guide wire.
Claim 14
Dependent Claim
39 words
The method of claim 10, wherein the guanidino forming agent is selected from the group consisting of S-ethylthiouronium bromide, S-ethylthiouronium chloride, O-methylisourea, O-methylisouronium sulfate, O-methylisourea hydrogen sulfate, S-methylisothiourea, 2-methyl-1-nitroisourea, aminoiminomethanesulfonic acid, cyanamide, cyanoguanide, dicyandiamide, 3,5-dimethyl-1-guanylpyrazole nitrate and 3,5-dimethyl pyrazole.
Claim 15
Dependent Claim
24 words
The method of claim 10, wherein the negatively charged moiety is selected from the group consisting of a phosphate, a sulphate and a carboxylate.
Claim 16
Dependent Claim
14 words
The method of claim 10, wherein the guanidino moiety is an arginine amino acid.
Claim 17
Dependent Claim
13 words
The method of claim 10, wherein the biomolecule is a naturally occurring biomolecule.
Claim 18
Dependent Claim
13 words
The method of claim 10, wherein the biomolecule is a chemically synthesized biomolecule.
Claim 19
Dependent Claim
90 words
The method of claim 10, wherein the biomolecule is selected from the group consisting of a globular protein, a cell attachment protein, a protein, a cell attachment peptide, a peptide, an anti-inflammatory agent, an antibody, an antigen, an immunoglobulin, an enzyme, a hormone, a growth factor, a neurotransmitter, a cytokine, a proteoglycan, a toxin, an antibiotic agent, an antibacterial agent, an antimicrobial agent, an antithrombotic agent, a polysaccharide, a carbohydrate, a fatty acid, a nucleic acid, a DNA segment, RNA segment, a lectin, a drug, a vitamin and a ligand.
Claim 20
Dependent Claim
31 words
The method of claim 10, wherein at least a portion of the surface forms at least one of a tube, a rod, a membrane, a balloon, a bag and a sheet.
Claim 21
Dependent Claim
93 words
The method of claim 10, wherein the medical device comprises at least one of a biocompatible material selected from the group consisting of metal, titanium, titanium alloys, tin-nickel alloys, shape memory alloys, aluminum oxide, platinum, platinum alloys, stainless steel, MP35N stainless steel, elgiloy, stellite, pyrolytic carbon, silver carbon, glassy carbon, polymer, polyamide, polycarbonate, polyether, polyester, polyolefin, polyethylene, polypropylene, polystyrene, polyurethane, polyvinyl chloride, polyvinylpyrrolidone, silicone elastomer, fluoropolymer, polyacrylate, polyisoprene, polytetrafluoroethylene, rubber, ceramic, hydroxapatite, human protein, human tissue, animal protein, animal tissue, bone, skin, teeth, collagen, laminin; elastin, fibrin, wood, cellulose, compressed carbon and glass.
Claim 22
Independent Claim
125 words
A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissue or fluids in or temporarily removed from a living mammalian subject, the method comprising: (a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising a negatively charged moiety; (b) providing a biomolecule, the biomolecule comprising an amine moiety; (c) combining the amine moiety with a guanidino forming agent to form a positively charged guanidino moiety; and (d) combining the positively charged guanidino moiety with the negatively charged moiety to form an ionic bond, the ionic bond immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.
Claim 23
Independent Claim
125 words
A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting bodily tissue or fluids in or temporarily removed from a living mammalian subject, the method comprising: (a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising a negatively charged moiety; (b) providing a biomolecule, the biomolecule comprising an amine moiety; (c) combining the amine moiety with a guanidino forming agent to form a positively charged guanidino moiety; and (d) combining the positively charged guanidino moiety with the negatively charged moiety to form an ionic bond, the ionic bond immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.
Claim 24
Dependent Claim
71 words
The method of claim 20, wherein the device is selected from the group consisting of a blood-contacting medical device, a tissue-contacting medical device, a bodily fluid-contacting medical device, an implantable medical device, an extracorporeal medical device, a blood oxygenator, a blood pump, tubing for carrying blood, an endoprosthesis medical device, a vascular graft, a stent, a pacemaker lead, a heart valve, temporary intravascular medical device, a catheter and a guide wire.
Claim 25
Dependent Claim
39 words
The method of claim 20, wherein the guanidino forming agent is selected from the group consisting of S-ethylthiouronium bromide, S-ethylthiouronium chloride, O-methylisourea, O-methylisouronium sulfate, O-methylisourea hydrogen sulfate, S-methylisothiourea, 2-methyl-1-nitroisourea, aminoiminomethanesulfonic acid, cyanamide, cyanoguanide, dicyandiamide, 3,5-dimethyl-1-guanylpyrazole nitrate and 3,5-dimethyl pyrazole.
Claim 26
Dependent Claim
24 words
The method of claim 20, wherein the negatively charged moiety is selected from the group consisting of a phosphate, a sulphate and a carboxylate.
Claim 27
Dependent Claim
14 words
The method of claim 20, wherein the guanidino moiety is an arginine amino acid.
Claim 28
Dependent Claim
13 words
The method of claim 20, wherein the biomolecule is a naturally occurring biomolecule.
Claim 29
Dependent Claim
13 words
The method of claim 20, wherein the biomolecule is a chemically synthesized biomolecule.
Claim 30
Dependent Claim
90 words
The method of claim 20, wherein the biomolecule is selected from the group consisting of a globular protein, a cell attachment protein, a protein, a cell attachment peptide, a peptide, an anti-inflammatory agent, an antibody, an antigen, an immunoglobulin, an enzyme, a hormone, a growth factor, a neurotransmitter, a cytokine, a proteoglycan, a toxin, an antibiotic agent, an antibacterial agent, an antimicrobial agent, an antithrombotic agent, a polysaccharide, a carbohydrate, a fatty acid, a nucleic acid, a DNA segment, RNA segment, a lectin, a drug, a vitamin and a ligand.
Claim 31
Dependent Claim
31 words
The method of claim 20, wherein at least a portion of the surface forms at least one of a tube, a rod, a membrane, a balloon, a bag and a sheet.
Claim 32
Dependent Claim
93 words
The method of claim 20, wherein the medical device comprises at least one of a biocompatible material selected from the group consisting of metal, titanium, titanium alloys, tin-nickel alloys, shape memory alloys, aluminum oxide, platinum, platinum alloys, stainless steel, MP35N stainless steel, elgiloy, stellite, pyrolytic carbon, silver carbon, glassy carbon, polymer, polyamide, polycarbonate, polyether, polyester, polyolefin, polyethylene, polypropylene, polystyrene, polyurethane, polyvinyl chloride, polyvinylpyrrolidone, silicone elastomer, fluoropolymer, polyacrylate, polyisoprene, polytetrafluoroethylene, rubber, ceramic, hydroxapatite, human protein, human tissue, animal protein, animal tissue, bone, skin, teeth, collagen, laminin, elastin, fibrin, wood, cellulose, compressed carbon and glass.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2002-07-26 | 2003-01-25 | 2003-07-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-07-26 | 2007-01-25 | 2007-07-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-07-26 | 2011-01-25 | 2011-07-27 | $7700.00 | Window Closed / Lapsed |