Osteogenic composition and implant containing same

Claim 3

The method of claim 1 wherein an osteogenic composition in viscous liquid form is provided.

Claim 4

The method of claim 1 wherein in step (b), the demineralized bone matrix is converted to a pulp.

Claim 5

The method of claim 1 wherein the demineralized bone matrix contains not greater than about 10 weight percent calcium.

Claim 6

The method of claim 3 wherein the demineralized bone matrix contains not greater than about 1 percent calcium.

Claim 7

The method of claim 5 wherein the demineralized bone matrix contains not greater than about 0.5 weight percent calcium.

Claim 8

The method of claim 1 wherein said heating step (c) is carried out at a temperature of from about 30° to 75° C.

Claim 9

The method of claim 7 wherein said heating step (c) is carried out at a temperature of from about 35° to about 60° C.

Claim 10

The method of claim 8 wherein said heating step (c) is carried out at a temperature of from about 40° to about 55° C.

Claim 11

The method of claim 1 wherein said heating step (c) is carried out for a period of from about 5 minutes to about 10 hours.

Claim 12

The method of claim 1 comprising the additional step of adding demineralized bone powder to said osteogenic composition.

Claim 13

The method of claim 1 comprising the additional step of adding to said osteogenic composition, at least one ingredient selected from the group consisting of antiviral agent, antimicrobial agent, antibiotic agent, amino acid, peptide, vitamin, inorganic element, hormone, endocrine, tissue, synthesizer, enzyme, polymer-cell scaffolding agent with parenchymal cells, angiogenic drug, polymeric drug carrier, collagen lattice, antigenic agent, cytoskeletal agent, cartilage fragment, living cell, natural extract, tissue transplant, bioadhesive, biologically active component, mesenchymal agent, bone digester, antitumor agent, cellular attractant, cellular attachment agent, immunosuppressant, nucleic acid and penetration enhancer.

Claim 14

The method of claim 1 comprising the additional step of applying the osteogenic composition to a surface of a surgical implant device such that the osteogenic composition adheres thereto.

Claim 15

The method of claim 13 wherein the surgical implant device is selected from the group consisting of donor bone tissue, an osteoprosthetic device, an orthopedic fracture wrapping and a collagen-coated microspherical filler.

Claim 16

The method of claim 1 additionally comprising the step of: d) treating the liquid composition produced in step (c) to obtain the osteogenic composition in solid form by adding organic solvent to the liquid composition, whereby the osteogenic composition precipitates out of solution in solid form.

Claim 17

The method of claim 15 additionally comprising the step of: titrating the liquid composition produced in step (c), before carrying out step (d), to remove excess acid.

Claim 18

The osteogenic composition of claim 15, additionally comprising the step of: further dissolving the precipitated solid composition from step (d) in solution, whereby the osteogenic composition is in injectable form.

Claim 19

The method of claim 17 wherein the osteogenic composition is provided in a form selected from the group consisting of a form suitable for delivery by percutaneous injection to a fracture delivery site, a form for direct injection into an osseous defect resulting from periodontal disease, and as a general injectable substance for stimulating bone growth to counteract bone loss due to injury or disease.

Claim 20

The method of claim 1 additionally comprising the step of d) treating the liquid composition produced in step (c) to obtain the osteogenic composition in solid form, by first titrating the liquid composition produced in step (c) to remove excess acid, then placing the titrated composition in a dialysis bag and then carrying out dialysis, whereby the osteogenic composition remains in the dialysis bag in solid form.

Claim 21

The method of claim 19 additionally comprising the step of further dissolving the osteogenic composition from step (d) in solution, whereby the osteogenic composition is in injectable form.

Claim 22

The method of claim 20 wherein the osteogenic composition is provided in a form selected from the group consisting of a form suitable for delivery by percutaneous injection to a fracture delivery site, a form for direct injection into an osseous defect resulting from periodontal disease, and as a general injectable substance for stimulating bone growth to counteract bone loss due to injury or disease.

Claim 23

The method of claim 1 wherein said acid-promoted cleavage step (b) is carried out simultaneously with said bone demineralizing step (a).

Claim 24

The method of claim 1 wherein said acid-promoted cleavage step (b) is separately carried out after said demineralizing step (a) has been completed.

Claim 25

The method of claim 1 wherein said heating step (c) is carried out simultaneously with or subsequent to said acid-promoted cleavage step (b).

Claim 26

The method of claim 1 comprising the additional step of adding hydroxyapatite to the osteogenic composition.

Claim 27

The method of claim 1 comprising the additional step of adding non-demineralized bone powder to the osteogenic composition.

Claim 28

The method of claim 1 comprising the additional step of adding to the osteogenic composition, at least one ingredient selected from the group consisting of bone morphogenic agents and growth factors.

Claim 29

The method of claim 27 comprising the additional step of adding to the osteogenic composition, at least one ingredient selected from the group consisting of platelet derived growth factor, basic fibroblast growth factor, transforming growth factor beta, insulin-like growth factor, and epidermal growth factor.

Claim 30

The method of claim 1 comprising the additional step of incorporating the osteogenic composition within a surgical implant device.

Claim 31

The method of claim 29 wherein the surgical implant device is selected from the group consisting of donor bone tissue, an osteoprosthetic device, an orthopedic fracture wrapping and a collagen-coated microspherical filler.

Claim 32

The method of claim 1 wherein the demineralized bone tissue is derived from the group consisting of cortical autogenic, cortical allogeneic, cortical xenogeneic, cancellous autogenic, cancellous allogeneic, cancellous xenogeneic, corticocancellous autogenic, corticocancellous allogeneic and corticocancellous xenogeneic bone.

Claim 33

The method of claim 1 wherein concentration of acid in step (b) is at least about 2.0N.