US5108923A
Expired / Lapsed
US
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Bioadhesives for cell and tissue adhesion
Assignee: Discovery Labware Inc
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Filing: 1987-04-03
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Granted: 1992-04-28
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Expires: 2007-04-03
Abstract
Abstract A cell culturing system, methods for the preparation thereof, and methods for affixing other biologically active moieties to a substrate are provided. Said cell culturing system comprises: a substrate; a coating thereon of a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: <IMAGE> <IMAGE> <IMAGE> +TR <IMAGE> wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; viable cells affixed to said coated substrate; and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.
Inventors
- 👤 Christine V. Benedict
- 👤 Paul T. Picciano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:03
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61L; A61L27/00; A61L27/36; A61L27/38; C12N2533/00; C12N2533/50
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
146 words
A method of affixing viable cells to a substrate for in vitro culture comprising (1) coating a substrate with a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: ##STR5## wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; (2) drying said coating on said substrate; (3) fixing said coating on said substrate; (4) rinsing said coated substrate to remove extraneous materials not firmly attached to said substrate; and (5) applying viable mammalian cells to said coated substrate, whereby said cells become affixed to said coated substrate and, in the presence of a nutritive environment, perform normal metabolic cell functions.
Claim 2
Independent Claim
146 words
A method of affixing viable cells to a substrate for in vitro culture comprising (1) coating a substrate with a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: ##STR5## wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; (2) drying said coating on said substrate; (3) fixing said coating on said substrate; (4) rinsing said coated substrate to remove extraneous materials not firmly attached to said substrate; and (5) applying viable mammalian cells to said coated substrate, whereby said cells become affixed to said coated substrate and, in the presence of a nutritive environment, perform normal metabolic cell functions.
Claim 3
Dependent Claim
38 words
A method as defined in claim 1 wherein said substrate is coated with from 0.1 to 2 μl of said formulation containing from about 10 to 60 μg/μl of said bioadhesive polyphenolic protein per cm 2 of substrate.
Claim 4
Dependent Claim
26 words
A method as defined in claim 1 wherein said coating is fixed on said substrate by rinsing said substrate with an anhydrous biologically compatible sterilizing medium.
Claim 5
Dependent Claim
16 words
A method as defined in claim 3 wherein said sterilizing medium is 30 to 100% ethanol.
Claim 6
Dependent Claim
14 words
A method as defined in claim 3 wherein said sterilizing medium is 30-100% isopropanol.
Claim 7
Dependent Claim
24 words
A method as defined in claim 1 wherein the coated substrate is rinsed to remove extraneous materials with sterile aqueous serum-free tissue culture medium.
Claim 8
Dependent Claim
22 words
A method as defined in claim 1 wherein the coated substrate is rinsed to remove extraneous materials with water or buffer solution.
Claim 9
Dependent Claim
21 words
A method as defined in claim 1 wherein the viable cells are applied to the coated substrate in a serum-containing medium.
Claim 10
Dependent Claim
21 words
A method as defined in claim 1 wherein the viable cells are applied to said coated substrate in a serum-free medium.
Claim 11
Dependent Claim
15 words
A method as defined in claim 1 wherein said substrate is an in vitro substrate.
Claim 12
Dependent Claim
25 words
A method as defined in claim 10 wherein said substrate is selected from the group consisting of synthetic polymeric materials, glass, metals, and microporous filters.
Claim 13
Dependent Claim
12 words
A method as defined in claim 11 wherein said substrate is polytetrafluoroethylene.
Claim 14
Dependent Claim
15 words
A method as defined in claim 11 wherein said substrate is an in vivo substrate.
Claim 15
Dependent Claim
25 words
A method as defined in claim 13 wherein the substrate is selected from the group consisting of collagen, laminin, fibronectin, prosthetic grafting materials and polylysine.
Claim 16
Dependent Claim
12 words
A method as defined in claim 14 wherein said substrate is polytetrafluoroethylene.
Claim 17
Dependent Claim
102 words
An in vitro cell culturing system comprising: a substrate; a coating thereon of a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: ##STR6## wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; viable mammalian cells affixed to said coated substrate; and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.
Claim 18
Dependent Claim
102 words
An in vitro cell culturing system comprising: a substrate; a coating thereon of a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: ##STR6## wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; viable mammalian cells affixed to said coated substrate; and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.
Claim 19
Dependent Claim
17 words
A cell culturing system as defined in claim 16 wherein the substrate is an in vitro substrate.
Claim 20
Dependent Claim
27 words
A cell culturing system as defined in claim 17 wherein the substrate is selected from the group consisting of synthetic polymeric material, glass, metals, and microporous filters.
Claim 21
Dependent Claim
14 words
A cell culturing system as defined in claim 18 wherein said substrate is polytetrafluoroethylene.
Claim 22
Dependent Claim
17 words
A cell culturing system as defined in claim 16 wherein said substrate is an in vivo substrate.
Claim 23
Dependent Claim
27 words
A cell culturing system as defined in claim 20 wherein the substrate is selected from the group consisting of collagen, laminin, fibronectin, prosthetic grafting materials and polylysine.
Claim 24
Dependent Claim
14 words
A cell culturing system as defined in claim 21 wherein the substrate is polytetrafluoroethylene.
Claim 25
Dependent Claim
28 words
A method as defined in claim 1, wherein the bioadhesive polyphenolic protein contains about 45% pure bioadhesive polyphenolic protein and is obtained from the marine mussel Mytilus edulis.
Claim 26
Dependent Claim
29 words
A cell culturing system according to claim 16, wherein the bioadhesive polyphenolic protein contains about 45% pure bioadhesive polyphenolic protein and is obtained from the marine mussel Mytilus edulis.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-04-28 | 1995-10-28 | 1996-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-04-28 | 1999-10-28 | 2000-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-04-28 | 2003-10-28 | 2004-04-28 | $7700.00 | Window Closed / Lapsed |