US3910819A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Treatment of surfaces to stimulate biological cell adhesion and growth
Assignee: California Institute of Technology
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Filing: 1974-02-19
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Granted: 1975-10-07
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Expires: 1994-02-19
Abstract
Abstract Contact of negatively charged biological cells is promoted by dispersion within the cell medium of a minor amount of a polyquaternary polymeric amine known as an ionene. Adhesion of the cells to normally negatively charged surfaces such as glass or plastic tissue culture devices is stimulated by pretreating the surfaces of the devices with a dilute solution of the ionene polymer. Increased cell adhesion and growth is demonstrated for both normal and transformed cells. Experiments have verified that cells grow at a more rapid rate on the surface of an ionene treated container than in the most effective treated containers that are commercially available.
Inventors
- 👤 Alan Rembaum
- 👤 Marylou Ingram
- 👤 F Agnes Stroud-Schmink
- 👤 Donald E Rounds
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 17:15
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N1/00; C12N1/38; C12N2533/00; C12N2533/50
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
70 words
A METHOD OF PROMOTING GROWTH OF BIOLOGICAL CELLS IN VITRO COMPRISING THE STEPS OF: ADDING TO A CULTURE MEDIUM OF THE CELLS A MINOR GROWTH PROMOTING AMOUNT OF A WATER-SOLUBLE, LINEAR, IONENE POLYQUATERNARY POLYELECTROLYTE POLYMER HAVING A MOLECULAR WEIGHT FROM 3,000 TO 100,000, AN AVERAGE CHARGE OF AT LEAST ONE INTRAPOLYMERIC QUATERNARY NITROGEN ATOM FOR AN AVERAGE OF EVERY TWELVE POLYMER CHAIN ATOMS AND A REPEATING UNIT OF THE FORMULA:
Claim 2
Independent Claim
70 words
A METHOD OF PROMOTING GROWTH OF BIOLOGICAL CELLS IN VITRO COMPRISING THE STEPS OF: ADDING TO A CULTURE MEDIUM OF THE CELLS A MINOR GROWTH PROMOTING AMOUNT OF A WATER-SOLUBLE, LINEAR, IONENE POLYQUATERNARY POLYELECTROLYTE POLYMER HAVING A MOLECULAR WEIGHT FROM 3,000 TO 100,000, AN AVERAGE CHARGE OF AT LEAST ONE INTRAPOLYMERIC QUATERNARY NITROGEN ATOM FOR AN AVERAGE OF EVERY TWELVE POLYMER CHAIN ATOMS AND A REPEATING UNIT OF THE FORMULA:
Claim 3
Dependent Claim
40 words
A method according to claim 1 in which the medium is cultured in a container and the ionene polyelectrolyte is applied as a dilute solution to the surface of the container in contact with the medium before contact is made.
Claim 4
Dependent Claim
16 words
A method according to claim 2 in which the surface is selected from glass and plastic.
Claim 5
Dependent Claim
12 words
A method according to claim 3 in which the plastic is polystyrene.
Claim 6
Dependent Claim
22 words
A method according to claim 3 in which the concentration of ionene polymer on the surface is at least 0.01 Mu g/cm2.
Claim 7
Dependent Claim
26 words
A method according to claim 1 in which the ionene polymer has a molecular weight from 10,000 to 60,000 and Z is chloro, bromo or iodo.
Claim 8
Dependent Claim
23 words
A method according to claim 1 in which R3 and R4 are alkylene of 3-6 carbon atoms and R1 and R2 are methyl.
Claim 9
Dependent Claim
15 words
A method according to claim 7 in which R3 is hexamethylene and R4 is decamethylene.
Claim 10
Dependent Claim
14 words
A method according to claim 7 in which R3 and R4 are both propylene.
Claim 11
Dependent Claim
20 words
A method according to claim 1 in which the concentration of ionene polymer is no greater than 4 Mu g/ml.
Claim 12
Independent Claim
73 words
An apparatus for culturing biological cells in which the cell contacting surface of the apparatus contains a growth promoting layer of a water-soluble, linear ionene polymer at a concentration of at least 0.01 Mu g/cm2; said ionene polymer having a molecular weight from 3,000 to 100,000, an average charge of at least one intrapolymeric quaternary nitrogen atom for an average of every twelve polymer chain atoms and a repeating unit of the formula:
Claim 13
Independent Claim
73 words
An apparatus for culturing biological cells in which the cell contacting surface of the apparatus contains a growth promoting layer of a water-soluble, linear ionene polymer at a concentration of at least 0.01 Mu g/cm2; said ionene polymer having a molecular weight from 3,000 to 100,000, an average charge of at least one intrapolymeric quaternary nitrogen atom for an average of every twelve polymer chain atoms and a repeating unit of the formula:
Claim 14
Dependent Claim
35 words
An apparatus in accordance with claim 11 in which the cell contacting surfaces are selected from glass and plastic, the ionene is a 6,10-ionene chloride and the concentration is no more than 0.1 Mu g/cm2.
Claim 15
Dependent Claim
57 words
A culture medium for promoting the growth of biological cells containing up to 4 Mu g/ml of a water-soluble, linear ionene polymer having a molecular weight from 3,000 to 100,000, an average charge of at least one intrapolymeric quaternary nitrogen atom for an average of every twelve polymer chain atoms and a repeating unit of the formula:
Claim 16
Dependent Claim
57 words
A culture medium for promoting the growth of biological cells containing up to 4 Mu g/ml of a water-soluble, linear ionene polymer having a molecular weight from 3,000 to 100,000, an average charge of at least one intrapolymeric quaternary nitrogen atom for an average of every twelve polymer chain atoms and a repeating unit of the formula:
References Cited (Backward Citations)
US3759788A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-10-06 | 1979-04-07 | 1979-10-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-10-06 | 1983-04-07 | 1983-10-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-10-06 | 1987-04-07 | 1987-10-07 | $7700.00 | Window Closed / Lapsed |