US4496397A
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Process for purifying and stabilizing catechol-containing proteins and materials obtained thereby
Assignee: CONNECTICUT UNIVERSITY OF CONNECTICUT HEALTH CENTER, University of
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Filing: 1984-03-07
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Granted: 1985-01-29
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Expires: 2004-03-07
Abstract
Abstract A process is provided for purifying and stabilizing marine mussel polyphenolic proteins rich in 3,4-dihydroxyphenylalanine (dopa) and hydroxyproline (hyp) while obtaining high yields thereof. The process includes the steps of providing an acid soluble extract of the dopa-containing proteins, removing the low molecular weight material from the extract and reacting the remaining proteinaceous material with a water soluble borate at a pH of 7.0-9.0 to provide a soluble borate complex of the dopa-containing protein while precipitating impurities. The complex is separated from the precipitate and may be concentrated for storage or treated with an acetic acid solution and either concentrated or lyophilized and stored under an inert atmosphere. The purified proteins exhibit a dopa:protein index of purity ratio of at least about 0.10.
Inventors
- 👤 J. Herbert Waite
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:02
CPC Classifications
C; C09; C09J; C09J189/00; A; A23; A23J; A23J1/00; A23J1/04; Y; Y10; Y10S; Y10S530/00; Y10S530/855; Y10S530/857
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
111 words
A process for purifying and stabilizing catechol-containing polyphenolic proteins comprising the steps of: (a) providing an acid soluble extract of a catechol-containing polyphenolic proteins in an acid medium; (b) removing from the extract those acid soluble components having a low average molecular weight to provide a remaining extract fraction of relatively higher molecular weight catechol-containing proteins; (c) reacting said remaining extract fraction with a soluble borate at a pH in the range of 7.0 to 9.0 to form selective soluble borate complexes of the catechol-containing proteins; and (d) separating the borate complexes while maintaining the pH between 7.0 and 9.0 to permit stabilized storage of the proteins and subsequent use thereof.
Claim 2
Dependent Claim
111 words
A process for purifying and stabilizing catechol-containing polyphenolic proteins comprising the steps of: (a) providing an acid soluble extract of a catechol-containing polyphenolic proteins in an acid medium; (b) removing from the extract those acid soluble components having a low average molecular weight to provide a remaining extract fraction of relatively higher molecular weight catechol-containing proteins; (c) reacting said remaining extract fraction with a soluble borate at a pH in the range of 7.0 to 9.0 to form selective soluble borate complexes of the catechol-containing proteins; and (d) separating the borate complexes while maintaining the pH between 7.0 and 9.0 to permit stabilized storage of the proteins and subsequent use thereof.
Claim 3
Dependent Claim
39 words
The process of claim 1 wherein the removal of low molecular weight components includes subjecting the extract to dialysis against a dilute acetic acid solution using a dialysis membrane having a molecular weight exclusion limit of 50,000 and less.
Claim 4
Dependent Claim
24 words
The process of claim 1 wherein each of the purification steps is carried out at a cold temperature of less than about 10° C.
Claim 5
Dependent Claim
43 words
The process of claim 1 wherein the reacting of the remaining extract fraction includes dialyzing the fraction against a large volume of a sodium borate solution for a period of time sufficient to complete at least about 90 percent of the complexing reaction.
Claim 6
Dependent Claim
26 words
The process of claim 1 wherein the water soluble borate reacted with the extract fraction has a molar concentration in the range of about 0.05-0.50 mole.
Claim 7
Dependent Claim
28 words
The process of claim 1 where the separation of the purified complexes includes subjecting the reaction mixture to centrifugation and collecting the supernatant liquid containing the stabilized complexes.
Claim 8
Dependent Claim
45 words
The process of claim 1 wherein the separated borate complexes are treated to reduce the pH to well below 7.0 to provide a purified acidic solution of the catechol-containing polyphenolic proteins and subsequently treating the acid solution to obtain the purified proteins for stabilized storage.
Claim 9
Dependent Claim
97 words
The process of claim 1 wherein the removal of low molecular weight materials includes subjecting the extract to dialysis against a large volume of a weak acid solution for a sufficient time to provide the remaining extract fraction; the reacting of the extract fraction includes dialyzing the fraction against a large volume of a borate solution at a pH in the range of about 7.5-9.0 and the separation of the complexes includes subjecting the reaction mixture to centrifugation for a brief period of time at high centrifugal force and collecting the supernatant liquid containing the stabilized complexes.
Claim 10
Dependent Claim
23 words
The process of claim 8 wherein each of the purification steps is carried out at a temperature of less than about 10° C.
Claim 11
Dependent Claim
47 words
The process of claim 8 wherein the separated borate complexes are treated to reduce the pH to a level well below 7.0 to provide a purified acidic solution of the catechol-containing polyphenolic proteins and subsequently treating the acid solution to obtain the purified proteins for stabilized storage.
Claim 12
Dependent Claim
64 words
The process of claim 1 wherein the separation of the purified complexes includes subjecting the reaction mixture to centrifugation while at a temperature of less than about 10° C. and at a pH in the range of about 7.5-9.0 and collecting the supernatant liquid containing the stabilized complexes, and subsequently concentrating the solution of catechol-containing proteins to obtain the purified proteins for stabilized storage.
Claim 13
Dependent Claim
26 words
The process of claim 11 wherein the collected borate complex-containing solution is treated to reduce the pH thereof to about 2.5-4 prior to concentrating the solution.
Claim 14
Dependent Claim
48 words
The process of claim 1 wherein the separated borate complexes are treated to reduce the pH to about 2.5-4 to provide a purified acidic solution of the catechol-containing polyphenolic proteins and subsequently lyophilizing the acid solution in an inert atmosphere to obtain the purified proteins in solid form.
Claim 15
Dependent Claim
56 words
The process of claim 1 wherein the separated borate complexes are treated to reduce the pH to about 2.5-4 by dialyzing the supernatant liquid containing the stabilized complexes against a large volume of a dilute acetic acid solution at a temperature of about 1°-8° C. to provide a purified acidic solution of the catechol-containing polyphenolic proteins.
Claim 16
Dependent Claim
147 words
The process of claim 1 wherein the removal of low molecular weight materials includes subjecting the extract to dialysis against at least about 500 volumes of a 1-5 percent acetic acid solution at a temperature of about 1°-8° C. using a dialysis membrane having a molecular weight cut off porosity of less than about 50,000 to provide the remaining extract fraction; the reacting of the remaining extract fraction includes dialyzing the fraction against at least about 500 volumes of a sodium borate solution at a pH in the ranqe of 7.5-8.5 at a temperature of 1°-8° C. for a period of time sufficient to complete at least about 90 percent of the complexing reaction; and the separation of the purified complexes includes subjected the reaction mixture to centrifugation of about 15,000-50,000 x g while at the same pH and collecting the supernatant liquid containing the stabilized complexes.
Claim 17
Dependent Claim
34 words
The process of claim 1 wherein the stabilized complexes are subsequently ooncentrated while maintaining the borate molarity at a level in excess of the molarity of the catechol by at least about 5-10 percent.
Claim 18
Dependent Claim
130 words
The process of claim 1 wherein the catechol is dopa, the removal of low molecular weight materials includes subjecting the extract to dialysis against an acetic acid solution at a temperature of about 4° C. to provide a remaining extract fraction; the reacting of the remaining extract fraction includes dialyzing the fraction against a sodium borate solution at a pH of about 8.0 and a temperature of about 4° C. for a period of time sufficient to complete about 95-98 percent of the complexing reaction and cause precipitation of selected noncomplexed proteins; the separation of the purified complexes includes subjecting the reaction mixture to centrifugation at about 30,000 x g while at a pH of about 8.0-8.5 and collecting the supernatant liquid containing the stabilized complexes of the dopa-containing proteins.
Claim 19
Dependent Claim
38 words
The process of claim 17 wherein the stabilized complexes of the dopa-containing proteins are subsequently concentrated while maintaining the borate molarity at a level in excess of the molarity of the dopa by at least about 5-10 percent.
Claim 20
Dependent Claim
79 words
The process of claim 17 wherein the stabilized complexes of the dopa-containing protein are treated to reduce the pH to about 2.5-4 by dialyzing the supernatant liquid containing the stabilized complexes against at least about 500 volumes of a 1-5 percent acetic acid solution at a temperature of about 1°-8° C. to provide a purified acidic solution of the dopa-containing polyphenolic proteins and lyophilizing the acidic solution under an inert atmosphere to obtain the purified proteins in solid form.
Claim 21
Dependent Claim
90 words
A purified and stabilized polyphenolic proteinaceous material comprising polypeptide proteins rich in 3,4 dihydroxyphenylalanine (dopa) and hydroxyproline (hyp) having a molecular weight in the range of about 110,000-140,000 with a repeated decapeptide having an amino acid sequence wherein dopa is sequenced predominantly at positions five and nine and hyp is sequenced predominantly at positions six and seven; said material having a dopa/protein index of purity ratio of at least about 0.10 and being free from collagens and other alkaline-insoluble proteins incapable of forming borate complexes at a pH of 7.0-9.0.
Claim 22
Dependent Claim
90 words
A purified and stabilized polyphenolic proteinaceous material comprising polypeptide proteins rich in 3,4 dihydroxyphenylalanine (dopa) and hydroxyproline (hyp) having a molecular weight in the range of about 110,000-140,000 with a repeated decapeptide having an amino acid sequence wherein dopa is sequenced predominantly at positions five and nine and hyp is sequenced predominantly at positions six and seven; said material having a dopa/protein index of purity ratio of at least about 0.10 and being free from collagens and other alkaline-insoluble proteins incapable of forming borate complexes at a pH of 7.0-9.0.
Claim 23
Dependent Claim
46 words
A solution of a borate complex of the purified and stabilized proteinaceous material of claim 20 wherein the solution has a pH in the range of 7.0-9.0 and the molarity level of borate in the solution exceeds that of dopa by at least about 5-10 percent.
Claim 24
Dependent Claim
18 words
The material of claim 20 where the proteinaceous material is in dry solid form under an inert atmosphere.
Claim 25
Dependent Claim
31 words
An acidic solution of the purified and stabilized proteinaceous material of claim 20 wherein the acid is acetic acid at an acid concentration of about 5 percent by weight and less.
Claim 26
Dependent Claim
19 words
The material of claim 20 wherein the decapeptide is repeated from 60 to 100 times within the polypeptide protein.
Claim 27
Dependent Claim
14 words
The purified and stabilized proteins obtained in accordance with the method of claim 1.
Claim 28
Dependent Claim
14 words
The purified and stabilized proteins obtained in accordance with the method of claim 8.
Claim 29
Dependent Claim
14 words
The purified and stabilized proteins obtained in accordance with the method of claim 11.
Claim 30
Dependent Claim
14 words
The purified and stabilized proteins obtained in accordance with the method of claim 17.
Claim 31
Dependent Claim
14 words
The purified and stabilized proteins obtained in accordance with the method of claim 19.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-29 | 1988-07-30 | 1989-01-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-29 | 1992-07-30 | 1993-01-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-29 | 1996-07-30 | 1997-01-29 | $7700.00 | Window Closed / Lapsed |