US4266032A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of culturing microcarrier supported cells
Assignee: Monsanto Co
•
Filing: 1979-08-10
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Granted: 1981-05-05
•
Expires: 1999-08-10
Abstract
Abstract A method of submerged cell culture is provided which employs a microcarrier support comprising cross-linked polystyrene resin beads derivatized with amino acids, peptides, or hydroxy carboxylic acids.
Inventors
- 👤 Robert E. Miller
- 👤 Mau-Jung Kuo
- 👤 Charles Lewis, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:32
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N5/0075; C12M; C12M25/00; C12M25/16; C12N2533/00; C12N2533/30
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
57 words
In the method of growing anchorage dependent cells in suspension the improvement comprising employing in the suspension as a microcarrier support for attachment of cells a cross-linked polystyrene resin derivatized by reaction with material selected from the group consisting of amino acid, peptide and hydroxy carboxylic acid and bonded thereto through a carboxyl group of said material.
Claim 2
Dependent Claim
57 words
In the method of growing anchorage dependent cells in suspension the improvement comprising employing in the suspension as a microcarrier support for attachment of cells a cross-linked polystyrene resin derivatized by reaction with material selected from the group consisting of amino acid, peptide and hydroxy carboxylic acid and bonded thereto through a carboxyl group of said material.
Claim 3
Dependent Claim
15 words
The method of claim 1 in which the resin is derivatized with an amino acid.
Claim 4
Dependent Claim
12 words
The method of claim 2 in which the amino acid is glycine.
Claim 5
Dependent Claim
12 words
The method of claim 2 in which the amino acid is proline.
Claim 6
Dependent Claim
14 words
The method of claim 4 in which the cells are human peripheral lymphocyte cells.
Claim 7
Dependent Claim
14 words
The method of claim 1 in which the cells are primary monkey kidney cells.
Claim 8
Dependent Claim
12 words
The method of claim 6 in which the amino acid is lysine.
Claim 9
Dependent Claim
12 words
The method of claim 6 in which the amino acid is serine.
Claim 10
Dependent Claim
27 words
The method of claim 2 in which the amount of amino acid in the microcarrier ranges from about 0.2 to about 0.6 millimoles per gram of microcarrier.
Claim 11
Dependent Claim
16 words
The method of claim 1 in which the resin is derivatized with a hydroxy carboxylic acid.
Claim 12
Dependent Claim
14 words
The method of claim 10 in which the hydroxy carboxylic acid is gluconic acid.
Claim 13
Dependent Claim
14 words
The method of claim 10 in which the hydroxy carboxylic acid is lactobionic acid.
Claim 14
Dependent Claim
14 words
The method of claim 10 in which the hydroxy carboxylic acid is glycolic acid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-05-04 | 1984-11-03 | 1985-05-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-05-04 | 1988-11-03 | 1989-05-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-05-04 | 1992-11-03 | 1993-05-05 | $7700.00 | Window Closed / Lapsed |