US5108753A
Expired / Lapsed
US
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Osteogenic devices
Assignee: Stryker Corp
•
Filing: 1990-09-07
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Granted: 1992-04-28
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Expires: 2010-09-07
Abstract
Abstract Disclosed are osteogenic devices comprising a matrix containing substantially pure mammalian osteogenic protein and methods of inducing endochondral bone growth in mammals. A partial amino acid sequence, amino acid composition, solubility properties, and various other data characterizing osteogenic protein are also disclosed, as well as a nucleic acid sequences encoding a consensus osteogenic protein.
Inventors
- 👤 Thangavel Kuberasampath
- 👤 David C. Rueger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 02:30
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/0012; A61K9/0019; A61K9/0024; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61L27/36; A61L27/3604; A61L27/3608; A61L27/50; A61L27/56; C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61C; A61C8/00; A61C8/0003; A61C8/0004; A61C8/0006; A61K38/00; A61L2430/00; A61L2430/02; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/84; Y10S623/00; Y10S623/902; Y10S623/908
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
96 words
An osteogenic device for implantation in a mammal, said device comprising: a biocompatible, porous, insoluble particulate matrix defining pores of a dimension sufficient to permit influx, differentiation, a proliferation of migratory progenitor cells from the body of said mammal; and substantially pure osteogenic protein disposed on the surface of said matrix, said protein comprising a pair of polypeptide chains disulfide bonded to produce a dimeric species having a conformation such that said pair of polypeptide chains is capable of inducing endochondral bone formation in said mammal when disposed in said matrix and accessible to said cells.
Claim 2
Dependent Claim
96 words
An osteogenic device for implantation in a mammal, said device comprising: a biocompatible, porous, insoluble particulate matrix defining pores of a dimension sufficient to permit influx, differentiation, a proliferation of migratory progenitor cells from the body of said mammal; and substantially pure osteogenic protein disposed on the surface of said matrix, said protein comprising a pair of polypeptide chains disulfide bonded to produce a dimeric species having a conformation such that said pair of polypeptide chains is capable of inducing endochondral bone formation in said mammal when disposed in said matrix and accessible to said cells.
Claim 3
Dependent Claim
22 words
The device of claim 1 wherein said matrix comprises close-packed particulate matter having a particle size within the range of 70-850 μm.
Claim 4
Dependent Claim
19 words
The device of claim 2 wherein said particulate matter has a particle size within the range of 70-420 μm.
Claim 5
Dependent Claim
14 words
The device of claim 1 wherein said matrix comprises demineralized, protein extracted, particulate bone.
Claim 6
Dependent Claim
40 words
The device of claim 1 wherein said matrix comprises a material selected from the group consisting of collagen, hydroxyapatite, tricalcium phosphate, polymers comprising lactic acid monomer units, polymers comprising glycolic acid monomer units, demineralized, guanidine-extracted allogenic bone, and mixtures thereof.
Claim 7
Dependent Claim
18 words
The device of claim 1 wherein said matrix is shaped to span a non-union fracture in said mammal.
Claim 8
Dependent Claim
13 words
The device of claim 1 disposed within the marrow cavity of allogeneic bone.
Claim 9
Dependent Claim
31 words
The device of claim 1 wherein said osteogenic protein has a molecular weight of about 30 kD when oxidized as determined by comparison to molecular weight standards in SDS-polyacrylamide gel electrophoresis.
Claim 10
Dependent Claim
11 words
The device of claim 1 wherein said osteogenic protein is glycosylated.
Claim 11
Dependent Claim
47 words
The device of claim 1 wherein one of said pair of polypeptide chains has a molecular weight of about 16 kD and the other has a molecular weight of about 18 kD, both as determined after reduction by comparison to molecular weight standards in SDS-polyacrylamide gel electrophoresis.
Claim 12
Dependent Claim
16 words
The device of claim 1 wherein said osteogenic protein comprises the following amino acid sequence: D-F-D-A-Y-Y-C-S-G-A-C-Q-F-P-S.
Claim 13
Dependent Claim
16 words
The device of claim 1 wherein said osteogenic protein comprises the following amino acid sequence: S-L-K-P-S-N-Y-A-T-I-Q-S-I-V.
Claim 14
Dependent Claim
16 words
The device of claim 1 wherein said osteogenic protein comprises the following amino acid sequence: E-L-S-A-I-S-M-L-Y-L-D-E-N.
Claim 15
Dependent Claim
16 words
The device of claim 1 wherein said osteogenic protein comprises the following amino acid sequence: M-S-S-L-S-I-L-F-F-D-E-N.
Claim 16
Dependent Claim
36 words
The method of inducing local cartilage and bone formation in a mammal comprising the step of implanting the device of claim 1 in said mammal at a locus accessible to migratory progenitor cells of said mammal.
Claim 17
Dependent Claim
34 words
A method of inducing endochondral bone formation in a mammal comprising the step of implanting the device of claim 1 in said mammal at a locus accessible to migratory progenitor cells of said mammal.
Claim 18
Dependent Claim
31 words
A method of inducing endochondral bone formation in a non union fracture in a mammal comprising the step of implanting in the fracture in said mammal the device of claim 6.
Claim 19
Dependent Claim
28 words
The device of claim 1 wherein said osteogenic protein has a half maximum activity of at least about 25 to 50 ng per 25 mg of said matrix.
Claim 20
Dependent Claim
14 words
The osteogenic device of claim 1 for use in periodontal or dental reconstructive procedures.
Claim 21
Dependent Claim
45 words
The method of claim 16 comprising the step of implanting the device of claim 1 in said mammal at a locus accessible to migratory progenitor cells of said mammal wherein said locus in a mammalian jaw bone for use in periodontal or dental reconstructive procedures.
Claim 22
Independent Claim
13 words
The method of claims 15 or 16 for use in craniofacial reconstructive procedures.
References Cited (Backward Citations)
US4294753A
Cited
US4455256A
Cited
US4394370A
Cited
US4434094A
Cited
EP0128041A2
Cited
EP0148155A2
Cited
US4563489A
Cited
WO1985005274A1
Cited
WO1986000526A1
Cited
EP0169016A2
Cited
US4563350A
Cited
EP0182483A1
Cited
US4795467A
Cited
GB2178447A
Cited
EP0212474A2
Cited
WO1988000205A1
Cited
US5013649A
Cited
US4877864A
Cited
WO1989009605A1
Cited
WO1989010409A1
Cited
WO1990003733A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-04-28 | 1995-10-28 | 1996-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-04-28 | 1999-10-28 | 2000-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-04-28 | 2003-10-28 | 2004-04-28 | $7700.00 | Window Closed / Lapsed |