Patent US4919659A successfully refreshed from Google Patents.
US4919659A
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Radio frequency plasma deposited polymers that enhance cell growth
Assignee: University of Washington
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Filing: 1987-12-21
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Granted: 1990-04-24
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Expires: 2007-12-21
Abstract
Abstract A method for enhancing bio-material capability for use as a supporting surface for cell culture is disclosed. The method requires exposure of the bio-material to a plasma polymerizable polymer gas, which includes oxygen-containing organic molecules. The material surfaces are then subjected to an RF plasma gas discharge in the presence of the gas, causing a deposition on the exposed material surfaces of a conformal overcoating of the polymer on the material. The deposition enhances fibronectin adsorption and hence cell attachment, spreading and cell growth. Preferred polymerizable gases are acetone, methanol, and ethylene oxide.
Inventors
- 👤 Thomas A. Horbett
- 👤 Buddy D. Ratner
- 👤 Joseph A. Chinn
- 👤 Yasmeen Haque
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:52
CPC Classifications
B; B05; B05D; B05D1/00; B05D1/62; A; A61; A61L; A61L27/00; A61L27/28; A61L27/34; A61L33/00; A61L33/0005; A61L33/0011; A61L33/0029; A61L33/0076; A61L33/0088; C; C03; C03C; C03C17/00; C03C17/28; C03C17/32; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/50; Y; Y10; Y10S; Y10S623/00; Y10S623/92; Y10S623/921
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
115 words
A method of depositing a polymeric overcoat layer on a bio-material wherein said deposited layer has an effective amount of oxygen-containing groups and an enhanced ability to absorb a protein layer on said deposited layer when exposed to a biological fluid, and wherein cell attachment, mass cell culture, cell growth or mass tissue culture on said deposited layer is enhanced, said method comprising: exposing a surface of said bio-material to an effective amount of a gas, said gas being plasma polymerizable and includes oxygen-containing organic molecules; and subjecting said surface, in the presence of said gas, to a plasma gas discharge wherein said enhanced protein-adsorbing surface layer is generated and attached to said exposed surface.
Claim 2
Independent Claim
115 words
A method of depositing a polymeric overcoat layer on a bio-material wherein said deposited layer has an effective amount of oxygen-containing groups and an enhanced ability to absorb a protein layer on said deposited layer when exposed to a biological fluid, and wherein cell attachment, mass cell culture, cell growth or mass tissue culture on said deposited layer is enhanced, said method comprising: exposing a surface of said bio-material to an effective amount of a gas, said gas being plasma polymerizable and includes oxygen-containing organic molecules; and subjecting said surface, in the presence of said gas, to a plasma gas discharge wherein said enhanced protein-adsorbing surface layer is generated and attached to said exposed surface.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein said adsorbed protein layer is rich in fibronectin.
Claim 4
Dependent Claim
23 words
The method of claim 1 wherein said gas is selected from the group consisting of acetone, methanol, ethylene oxide, glutaraldehyde and mixtures thereof.
Claim 5
Dependent Claim
50 words
The method of claim 1 wherein said surface, after having been subjected to said plasma gas discharge, is further treated by exposing said surface to a biological fluid wherein a protein layer that enhances cell attachment, mass cell culture, cell growth and mass tissue culture is adsorbed by said surface.
Claim 6
Dependent Claim
16 words
The method of claim 4 wherein said protein is fibronectin and said biological fluid includes heparin.
Claim 7
Dependent Claim
20 words
The method of claim 1 wherein said material is selected from the group consisting of polymers, ceramics, glasses and metals.
Claim 8
Dependent Claim
23 words
The method of claim 6 wherein said polymer is selected from the group consisting of polyethylene, polyesters, polyacrylics, polyurethane, polystyrene, and silicon-containing polymers.
Claim 9
Dependent Claim
15 words
The method of claim 1 wherein said plasma gas deposition is generated by radio-frequency means.
Claim 10
Dependent Claim
16 words
The method of claim 1 wherein said plasma gas deposition is generated by microwave frequency means.
Claim 11
Dependent Claim
42 words
The method of claim 1 wherein said modified material surface characteristics result from said plasma-deposited polymer forming a conformal, polymeric overcoating on said material, said modified surfaces further characterized as being polar in nature and including oxygen-containing groups pendent upon said surface.
Claim 12
Dependent Claim
67 words
A biological implant article that promotes endothelial cell growth and heparin binding, said article consisting essentially of: a porous polymer material selected from the group consisting of polyester, tetrafluoroethylene and polyurethane; and an overcoated surface layer covalently bound to said porous polymer material and comprising a plasma gas discharge layer of gas polymer selected from the group consisting of acetone, methanol, ethylene oxide, gluteraldehyde and mixtures thereof.
Claim 13
Dependent Claim
67 words
A biological implant article that promotes endothelial cell growth and heparin binding, said article consisting essentially of: a porous polymer material selected from the group consisting of polyester, tetrafluoroethylene and polyurethane; and an overcoated surface layer covalently bound to said porous polymer material and comprising a plasma gas discharge layer of gas polymer selected from the group consisting of acetone, methanol, ethylene oxide, gluteraldehyde and mixtures thereof.
Claim 14
Dependent Claim
63 words
A composite support member for cell culture comprising a body of bio-material and a plasma-polymerized surface deposit layer thereon, said surface deposit layer consisting essentially of a plasma-polymerized deposit layer from and effective amount of a plasma-polymerizable organic gas, wherein said gas includes oxygen-containing organic molecules, and covalently bonded to the surface of said bio-material through pendent oxygen-containing groups of the plasma-polymerizable gas.
Claim 15
Dependent Claim
63 words
A composite support member for cell culture comprising a body of bio-material and a plasma-polymerized surface deposit layer thereon, said surface deposit layer consisting essentially of a plasma-polymerized deposit layer from and effective amount of a plasma-polymerizable organic gas, wherein said gas includes oxygen-containing organic molecules, and covalently bonded to the surface of said bio-material through pendent oxygen-containing groups of the plasma-polymerizable gas.
Claim 16
Dependent Claim
22 words
A support member according to claim 12 wherein said bio-material is selected from the group consisting of polymers, ceramics, glasses, and metals.
Claim 17
Dependent Claim
25 words
A support member according to claim 13 wherein said polymer is selected from the group consisting if polyethylenes, polyesters, polyacrylics, polyurethanes, polystyrenes, and silicon-containing polymers.
Claim 18
Dependent Claim
25 words
A support member according to claim 12 wherein said gas is selected from the group consisting of acetone, methanol, ethylene oxide, glutaraldehyde, and mixtures thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-04-23 | 1993-10-23 | 1994-04-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-04-23 | 1997-10-23 | 1998-04-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-04-23 | 2001-10-23 | 2002-04-24 | $7700.00 | Window Closed / Lapsed |