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US3941661A Expired / Lapsed US ⏱️ 0.0 years remaining

Roller culture bottle insert

Assignee: University of Missouri–St. Louis
•
Filing: 1974-01-16
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Granted: 1976-03-02
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Expires: 1994-01-16
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Abstract

Abstract A plastic spiral insert for use in existing roller culture bottles effective to increase the total surface area available for cell culture. The insert consists of a continuous coil of plastic sheet material that may be thermally set so as to retain its spiral configuration within the roller bottle and may also include integral raised portions on the sheet material effective to maintain uniform spacing between successive layers of the coil. The invention also includes retraction apparatus for removing the insert from small necked bottles.

Inventors

  • 👤 William D. Noteboom

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 15:06
CPC Classifications
C; C12; C12M; C12M27/00; C12M27/10; C12M27/12; C12M23/00; C12M23/02; C12M23/08; C12M27/18

Claims Analysis

Total Claims: 6
Claim 1
Independent Claim 115 words
Apparatus for use in a roller culture bottle that is partially filled with a culture medium for the growing of cells or microorganisms and comprising: a continuous elongated sheet of plastic material wound into a tight cylindrical coil and thermally set so as to retain a spiral configuration for insertion into the culture bottle and further adapted to expand into an enlarged spiral configuration after insertion into the bottle, and spacing means formed integrally with said sheet for separating successive layers of said spiral when present within the culture bottle so as to permit the passage of culture medium therethrough as the bottle is rolled about an axis coincident with the axis of said spiral.
Claim 2
Dependent Claim 115 words
Apparatus for use in a roller culture bottle that is partially filled with a culture medium for the growing of cells or microorganisms and comprising: a continuous elongated sheet of plastic material wound into a tight cylindrical coil and thermally set so as to retain a spiral configuration for insertion into the culture bottle and further adapted to expand into an enlarged spiral configuration after insertion into the bottle, and spacing means formed integrally with said sheet for separating successive layers of said spiral when present within the culture bottle so as to permit the passage of culture medium therethrough as the bottle is rolled about an axis coincident with the axis of said spiral.
Claim 3
Dependent Claim 38 words
The apparatus of claim 1 wherein, said sheet of plastic material is formed with a plurality of integral raised bosses, each adapted to contact a successive layer of said spiral for maintaining a uniform spacing between successive layers.
Claim 4
Dependent Claim 23 words
The apparatus of claim 1 wherein, said sheet of plastic material is formed on at least one edge with a continuous integral loop.
Claim 5
Dependent Claim 17 words
The apparatus of claim 1 wherein, said sheet of plastic material is comprised of open porous material.
Claim 6
Dependent Claim 31 words
The apparatus of claim 1 wherein, said sheet of plastic material is formed on its inner leading edge with integral engagement means to facilitate removal of said sheet from the bottle.

References Cited (Backward Citations)

US3633753A Cited
US3740321A Cited
US3695443A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1979-03-02 1979-09-01 1980-03-02 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1983-03-02 1983-09-01 1984-03-02 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1987-03-02 1987-09-01 1988-03-02 $7700.00 Window Closed / Lapsed

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