US6911212B2 — Malleable putty and flowable paste with allograft…

Claim 1

Independent Claim 131 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site comprising a mixture of partially demineralized osteoinductive and osteoconductive bone material having a calcium content ranging from above 4% to about 8% with a particle size ranging from about 100 to about 800 microns, the bone material ranging from about 25 to about 35% of the weight of the composition, the carrier comprising a hydrogel component in a phosphate buffered aqueous solution, said hydrogel component comprising sodium hyaluronate and its derivatives, and having a high molecular weight ranging from seven hundred thousand to three million Daltons with a stable viscosity from 22° to about 37° C. and ranging from about 0.75% to about 5.0% by weight of the aqueous carrier solution.

Claim 2

Dependent Claim 131 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site comprising a mixture of partially demineralized osteoinductive and osteoconductive bone material having a calcium content ranging from above 4% to about 8% with a particle size ranging from about 100 to about 800 microns, the bone material ranging from about 25 to about 35% of the weight of the composition, the carrier comprising a hydrogel component in a phosphate buffered aqueous solution, said hydrogel component comprising sodium hyaluronate and its derivatives, and having a high molecular weight ranging from seven hundred thousand to three million Daltons with a stable viscosity from 22° to about 37° C. and ranging from about 0.75% to about 5.0% by weight of the aqueous carrier solution.

Claim 3

Dependent Claim 26 words

A malleable bone composition as claimed in claim 1 wherein said mixture includes bone morphogenic protein in excess of the amount naturally occurring in allogeneic bone.

Claim 4

Dependent Claim 21 words

A malleable bone composition as claimed in claim 1 wherein said osteoconductive bone material has a calcium content greater than 4%.

Claim 5

Dependent Claim 26 words

A malleable bone composition as claimed in claim 1 wherein said phosphate includes two phosphate ions HPO 4 −2 and H 2 PO 4 −1 .

Claim 6

Dependent Claim 20 words

A malleable bone composition as claimed in claim 1 wherein said composition has an isotonic state of about 300 mOsmol/Kg.

Claim 7

Dependent Claim 18 words

A malleable bone composition as claimed in claim 1 wherein said bone powder contains cortical cancerous bone material.

Claim 8

Dependent Claim 22 words

A malleable bone composition as claimed in claim 1 wherein said composition has a pH ranging from about 6.8 to about 7.4.

Claim 9

Dependent Claim 33 words

A malleable bone composition as claimed in claim 1 wherein said bone material includes cortical cancellous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 10

Dependent Claim 37 words

A malleable bone composition as claimed in claim 1 wherein said bone powder contains growth factors such as transforming growth factor (TGF-beta), platelet derived growth factor (PDGF), osteopontin, fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1).

Claim 11

Dependent Claim 27 words

A malleable bone composition as claimed in claim 1 wherein said bone powder contains living cells such as blood cells, bone marrow cells and mesenchymal stem cells.

Claim 12

Dependent Claim 136 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing mixture of partially demineralized lyophilized allograft bone powder having a residual calcium content ranging from about 4% to about 8% with a particle size ranging from about 100 to about 800 microns in a sodium hyaluronate with an ionic salt based phosphate buffer carrier causing the composition to have a pH ranging between about 6.8 and about 7.4, the sodium hyaluronate component having a molecular weight of at least seven hundred Daltons and a stable viscosity ranging from 6,000 to about 275,000 cps at 22° to 37° C., the bone content of the carrier ranging in weight from about 20% to less than 50% total weight of the composition.

Claim 13

Dependent Claim 136 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing mixture of partially demineralized lyophilized allograft bone powder having a residual calcium content ranging from about 4% to about 8% with a particle size ranging from about 100 to about 800 microns in a sodium hyaluronate with an ionic salt based phosphate buffer carrier causing the composition to have a pH ranging between about 6.8 and about 7.4, the sodium hyaluronate component having a molecular weight of at least seven hundred Daltons and a stable viscosity ranging from 6,000 to about 275,000 cps at 22° to 37° C., the bone content of the carrier ranging in weight from about 20% to less than 50% total weight of the composition.

Claim 14

Dependent Claim 21 words

A malleable bone composition as claimed in claim 11 wherein said saline phosphate buffer carrier includes a sodium based phosphate compound.

Claim 15

Dependent Claim 28 words

A malleable bone composition as claimed in claim 11 wherein said phosphate includes at least two phosphate ions HPO 4 −2 and H 2 PO 4 −1 .

Claim 16

Dependent Claim 20 words

A malleable bone composition as claimed in claim 11 wherein said composition has an isotonic state of about 300 mOsmol/Kg.

Claim 17

Dependent Claim 20 words

A malleable bone composition as claimed in claim 1 wherein said bone powder has a residual calcium content above 4%.

Claim 18

Dependent Claim 16 words

A malleable bone composition as claimed in claim 11 wherein said sodium hyaluronate has been irradiated.

Claim 19

Dependent Claim 34 words

A malleable bone composition as claimed in claim 11 including antimicrobial and/or antibiotics such as erythromycin, bacitracin, neomycin, penicillin, polymyxin B, tetracycline, viomycin, chloromycetin and streptomycin, cefazolin, ampicillin, azactam, tobramycin, clindamycin, gentamycin and vitamins.

Claim 20

Dependent Claim 26 words

A malleable bone composition as claimed in claim 11 wherein said composition includes bone morphogenic proteins in excess of the amount naturally occurring in allogeneic bone.

Claim 21

Dependent Claim 20 words

A malleable bone composition as claimed in claim 11 wherein said composition has a pH ranging from 6.8 to 7.4.

Claim 22

Dependent Claim 37 words

A malleable bone composition as claimed in claim 11 wherein said bone powder contains growth factors such as transforming growth factor (TGF-beta), platelet derived growth factor (PDGF), osteopontin, fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1).

Claim 23

Dependent Claim 27 words

A malleable bone composition as claimed in claim 11 wherein said bone powder contains living cells such as blood cells, bone marrow cells and mesenchymal stem cells.

Claim 24

Dependent Claim 107 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site comprising a new bone growth inducing partially demineralized lyophilized allograft bone powder with a residual calcium content of about 4% to 5% and a particle size ranging from about 100 to about 800 microns in a high molecular weight sodium hyaluronate and saline phosphate buffer carrier, the bone content of the composition ranging from about 30% to about 35% by weight with the sodium hyaluronate component constituting a percentage of the carrier not in excess of 5% and has a molecular weight greater than 700,000 Daltons.

Claim 25

Dependent Claim 107 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site comprising a new bone growth inducing partially demineralized lyophilized allograft bone powder with a residual calcium content of about 4% to 5% and a particle size ranging from about 100 to about 800 microns in a high molecular weight sodium hyaluronate and saline phosphate buffer carrier, the bone content of the composition ranging from about 30% to about 35% by weight with the sodium hyaluronate component constituting a percentage of the carrier not in excess of 5% and has a molecular weight greater than 700,000 Daltons.

Claim 26

Dependent Claim 22 words

A malleable bone composition as claimed in claim 22 wherein said composition has a pH ranging from about 7.2 to about 7.4.

Claim 27

Dependent Claim 20 words

A malleable bone composition as claimed in claim 22 wherein said composition has an isotonic state of about 300 mOsmol/Kg.

Claim 28

Dependent Claim 37 words

A malleable bone composition as claimed in claim 22 wherein said bone powder contains growth factors such as transforming growth factor (TGF-beta), platelet derived growth factor (PDGF), osteopontin, fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1).

Claim 29

Dependent Claim 27 words

A malleable bone composition as claimed in claim 22 wherein said bone powder contains living cells such as blood cells, bone marrow cells and mesenchymal stem cells.

Claim 30

Dependent Claim 137 words

A sterile malleable bone gel composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized allograft bone material with a residual calcium content of above 4% to about 6% dry weight and a particle size ranging from about 100 to about 800 microns in a high molecular weight sodium hyaluronate having a molecular weight of at least about seven hundred thousand Daltons in saline phosphate buffer carrier with the sodium hyaluronate component comprising about 1.0% to about 4.0% of the carrier and having a viscosity of about 1,800 to 13,000 cps, the bone powder content of the composition ranging from about 25% to about 30% with said composition having an osmolality ranging from about 280 to about 340 mOsmol/Kg.

Claim 31

Dependent Claim 137 words

A sterile malleable bone gel composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized allograft bone material with a residual calcium content of above 4% to about 6% dry weight and a particle size ranging from about 100 to about 800 microns in a high molecular weight sodium hyaluronate having a molecular weight of at least about seven hundred thousand Daltons in saline phosphate buffer carrier with the sodium hyaluronate component comprising about 1.0% to about 4.0% of the carrier and having a viscosity of about 1,800 to 13,000 cps, the bone powder content of the composition ranging from about 25% to about 30% with said composition having an osmolality ranging from about 280 to about 340 mOsmol/Kg.

Claim 32

Dependent Claim 26 words

A malleable bone composition as claimed in claim 27 wherein said composition includes bone morphogenic proteins in excess of the amount naturally occurring in allogeneic bone.

Claim 33

Dependent Claim 26 words

A malleable bone composition as claimed in claim 27 wherein said phosphate includes two phosphate ions HPO 4 −2 and H 2 PO 4 −1 .

Claim 34

Dependent Claim 20 words

A malleable bone composition as claimed in claim 27 wherein said composition has an isotonic state of about 300 mOsmol/Kg.

Claim 35

Dependent Claim 18 words

A malleable bone composition as claimed in claim 27 wherein said bone material contains cortical allograft bone powder.

Claim 36

Dependent Claim 19 words

A malleable bone composition as claimed in claim 27 wherein said bone material includes cortical cancerous allograft bone powder.

Claim 37

Dependent Claim 22 words

A malleable bone composition as claimed in claim 27 wherein said composition has a pH ranging from about 7.2 to about 7.4.

Claim 38

Dependent Claim 33 words

A malleable bone composition as claimed in claim 27 wherein said bone material includes cortical cancerous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 39

Dependent Claim 37 words

A malleable bone composition as claimed in claim 27 wherein said bone powder contains growth factors such as transforming growth factor (TGF-beta), platelet derived growth factor (PDGF), osteopontin, fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1).

Claim 40

Dependent Claim 27 words

A malleable bone composition as claimed in claim 27 wherein said bone powder contains living cells such as blood cells, bone marrow cells and mesenchymal stem cells.

Claim 41

Dependent Claim 98 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a hydrogel ionic salt based phosphate buffer carrier, a hydrogel component comprising a glycosaminoglycan ranging from about 2.0% to about 5.0% by weight of the carrier and having a molecular weight of at least 700,000 Daltons.

Claim 42

Dependent Claim 98 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a hydrogel ionic salt based phosphate buffer carrier, a hydrogel component comprising a glycosaminoglycan ranging from about 2.0% to about 5.0% by weight of the carrier and having a molecular weight of at least 700,000 Daltons.

Claim 43

Dependent Claim 18 words

A malleable bone composition as claimed in claim 37 wherein said glycosaminoglycan is sodium hyaluronate and its derivatives.

Claim 44

Dependent Claim 28 words

A sterile malleable bone putty composition as claimed in claim 37 wherein said carrier has a viscosity ranging from 120,000 to 275,000 cps at 22° to 37° C.

Claim 45

Dependent Claim 115 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized allograft bone powder having a residual calcium content of about 4% dry weight in a high molecular weight hydrogel in saline phosphate buffer solution carrier, said hydrogel comprising a glycosaminoglycan having a molecular weight of at least 700,000 Daltons, the bone amount content of the composition ranging from about 27% to about 33% by weight and the high molecular weight hydrogel ranges from about 2.0% to about 5.0% by weight of the carrier, said composition having an osmolality ranging from 280-340 mOsmol/Kg.

Claim 46

Dependent Claim 115 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized allograft bone powder having a residual calcium content of about 4% dry weight in a high molecular weight hydrogel in saline phosphate buffer solution carrier, said hydrogel comprising a glycosaminoglycan having a molecular weight of at least 700,000 Daltons, the bone amount content of the composition ranging from about 27% to about 33% by weight and the high molecular weight hydrogel ranges from about 2.0% to about 5.0% by weight of the carrier, said composition having an osmolality ranging from 280-340 mOsmol/Kg.

Claim 47

Dependent Claim 29 words

A sterile malleable bone putty composition as claimed in claim 40 wherein said carrier has a stable viscosity ranging from 120,000 to 275,000 cps at 22° to 37° C.

Claim 48

Dependent Claim 30 words

A sterile malleable bone putty composition as claimed in claim 40 wherein said carrier has a stable viscosity ranging from 6,000 to about 275,000 cps at 22° to 37° C.

Claim 49

Dependent Claim 155 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing mixture of partially demineralized lyophilized allograft bone powder having a residual calcium content ranging from greater than 4% to about 8% with a particle size ranging from about 100 to about 800 microns in a hydrogel with an ionic salt based phosphate buffer carrier causing the composition to have a pH ranging between about 7.2 and about 7.4, said hydrogel comprising a polysaccharide selected from the group consisting of sodium hyaluronate, chitosan, sodium alginate, carboxymethylcellulose and hydroxypropylmethylcellulose, said hydrogel component having a molecular weight of at least twenty thousand Daltons and a stable viscosity ranging from 6,000 to about 275,000 cps at 22° to 37° C., the bone content of the carrier ranging in weight from about 20% to less than 50% total weight of the composition.

Claim 50

Dependent Claim 155 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing mixture of partially demineralized lyophilized allograft bone powder having a residual calcium content ranging from greater than 4% to about 8% with a particle size ranging from about 100 to about 800 microns in a hydrogel with an ionic salt based phosphate buffer carrier causing the composition to have a pH ranging between about 7.2 and about 7.4, said hydrogel comprising a polysaccharide selected from the group consisting of sodium hyaluronate, chitosan, sodium alginate, carboxymethylcellulose and hydroxypropylmethylcellulose, said hydrogel component having a molecular weight of at least twenty thousand Daltons and a stable viscosity ranging from 6,000 to about 275,000 cps at 22° to 37° C., the bone content of the carrier ranging in weight from about 20% to less than 50% total weight of the composition.

Claim 51

Dependent Claim 58 words

A sterile malleable bone putty composition as claimed in claim 43 wherein the bone amount content of the composition ranges from about 30% to about 38% by weight and the high molecular weight hydrogel ranges from about 2.0% to about 5.0% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 52

Dependent Claim 124 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized human allograft bone material having a residual calcium content of at least 4% to about 8% in a high molecular weight hydrogel and saline phosphate buffer solution carrier, said hydrogel comprising a sodium hyaluronate or its derivatives having a molecular weight of at least 3,000,000 Daltons, the bone amount content of the composition ranging from about 25% to about 35% by weight and the high molecular weight hydrogel ranging from about 0.25% to about 1.5% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 53

Dependent Claim 124 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized human allograft bone material having a residual calcium content of at least 4% to about 8% in a high molecular weight hydrogel and saline phosphate buffer solution carrier, said hydrogel comprising a sodium hyaluronate or its derivatives having a molecular weight of at least 3,000,000 Daltons, the bone amount content of the composition ranging from about 25% to about 35% by weight and the high molecular weight hydrogel ranging from about 0.25% to about 1.5% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 54

Dependent Claim 35 words

A sterile malleable bone putty composition as claimed in claim 45 wherein said bone material includes cortical cancellous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 55

Dependent Claim 122 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized human allograft bone material having a residual calcium content of at least 4% to about 8% mixed in a hydrogel and saline solution carrier, said hydrogel selected from a group consisting of chitosan and sodium alginate having a molecular weight ranging from 100,000 to about 300,000 Daltons, the bone amount content of the composition ranging from about 25% to about 50% by weight and the hydrogel ranging from about 1.0% to about 5.0% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 56

Dependent Claim 122 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of partially demineralized lyophilized human allograft bone material having a residual calcium content of at least 4% to about 8% mixed in a hydrogel and saline solution carrier, said hydrogel selected from a group consisting of chitosan and sodium alginate having a molecular weight ranging from 100,000 to about 300,000 Daltons, the bone amount content of the composition ranging from about 25% to about 50% by weight and the hydrogel ranging from about 1.0% to about 5.0% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 57

Dependent Claim 34 words

A sterile malleable bone composition as claimed in claim 47 wherein said bone material includes cortical cancellous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 58

Dependent Claim 112 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of demineralized lyophilized human allograft bone material having a residual calcium content of about 4% to about 8% in a hydrogel and saline phosphate buffer solution carrier, said hydrogel comprising a Dextran having a molecular weight of at least 40,000 Daltons, the bone amount content of the composition ranging from about 25% to about 50% by weight and the hydrogel ranging from about 10.0% to about 20.0% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 59

Dependent Claim 112 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of demineralized lyophilized human allograft bone material having a residual calcium content of about 4% to about 8% in a hydrogel and saline phosphate buffer solution carrier, said hydrogel comprising a Dextran having a molecular weight of at least 40,000 Daltons, the bone amount content of the composition ranging from about 25% to about 50% by weight and the hydrogel ranging from about 10.0% to about 20.0% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 60

Dependent Claim 34 words

A sterile malleable bone composition as claimed in claim 49 wherein said bone material includes cortical cancerous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 61

Dependent Claim 26 words

A sterile malleable bone composition as claimed in claim 49 wherein said bone material has a particle size ranging from about 100 to about 800 microns.

Claim 62

Dependent Claim 119 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of demineralized lyophilized human allograft bone material having a residual calcium content of about 4% to about 8% in a hydrogel and saline phosphate buffer solution carrier, said hydrogel selected from a group consisting of hydroxypropylmethylcellulose and carboxymethylcellulose having a molecular weight ranging from 20,000 to 100,000 Daltons, the bone amount content of the composition ranging from about 25% to about 35% by weight and the hydrogel ranging from about 4.0% to about 6.0% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 63

Dependent Claim 119 words

A sterile malleable bone composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing amount of demineralized lyophilized human allograft bone material having a residual calcium content of about 4% to about 8% in a hydrogel and saline phosphate buffer solution carrier, said hydrogel selected from a group consisting of hydroxypropylmethylcellulose and carboxymethylcellulose having a molecular weight ranging from 20,000 to 100,000 Daltons, the bone amount content of the composition ranging from about 25% to about 35% by weight and the hydrogel ranging from about 4.0% to about 6.0% by weight of the carrier, said composition having a pH ranging from about 7.2 to about 7.4.

Claim 64

Dependent Claim 34 words

A sterile malleable bone composition as claimed in claim 52 wherein said bone material includes cortical cancerous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 65

Dependent Claim 26 words

A sterile malleable bone composition as claimed in claim 52 wherein said bone material has a particle size ranging from about 100 to about 800 microns.

Claim 66

Dependent Claim 84 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a gelatin ionic salt based phosphate buffer carrier, the gelatin ranging from about 4.0% to about 6.0% by weight of the carrier.

Claim 67

Dependent Claim 84 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a gelatin ionic salt based phosphate buffer carrier, the gelatin ranging from about 4.0% to about 6.0% by weight of the carrier.

Claim 68

Dependent Claim 22 words

A malleable bone composition as claimed in claim 55 wherein said composition has a pH ranging from about 7.2 to about 7.4.

Claim 69

Dependent Claim 33 words

A malleable bone composition as claimed in claim 55 wherein said bone material includes cortical cancerous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 70

Dependent Claim 37 words

A malleable bone composition as claimed in claim 55 wherein said bone powder contains growth factors such as transforming growth factor (TGF-beta), platelet derived growth factor (PDGF), osteopontin, fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1).

Claim 71

Dependent Claim 27 words

A malleable bone composition as claimed in claim 55 wherein said bone powder contains living cells such as blood cells, bone marrow cells and mesenchymal stem cells.

Claim 72

Dependent Claim 80 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a collagen ionic salt based phosphate buffer carrier, the collagen being about 4.0% by weight of the carrier.

Claim 73

Dependent Claim 80 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a collagen ionic salt based phosphate buffer carrier, the collagen being about 4.0% by weight of the carrier.

Claim 74

Dependent Claim 22 words

A malleable bone composition as claimed in claim 60 wherein said composition has a pH ranging from about 7.2 to about 7.4.

Claim 75

Dependent Claim 33 words

A malleable bone composition as claimed in claim 60 wherein said bone material includes cortical cancerous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 76

Dependent Claim 37 words

A malleable bone composition as claimed in claim 60 wherein said bone powder contains growth factors such as transforming growth factor (TGF-beta), platelet derived growth factor (PDGF), osteopontin, fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1).

Claim 77

Dependent Claim 27 words

A malleable bone composition as claimed in claim 60 wherein said bone powder contains living cells such as blood cells, bone marrow cells and mesenchymal stem cells.

Claim 78

Dependent Claim 93 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a gelatin and collagen ionic salt based phosphate buffer carrier, the gelatin being about 6.0% by weight of the carrier and the collagen being about 4.0% by weight of the carrier.

Claim 79

Dependent Claim 93 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a particle size ranging from about 100 to about 800 microns in a gelatin and collagen ionic salt based phosphate buffer carrier, the gelatin being about 6.0% by weight of the carrier and the collagen being about 4.0% by weight of the carrier.

Claim 80

Dependent Claim 22 words

A malleable bone composition as claimed in claim 65 wherein said composition has a pH ranging from about 7.2 to about 7.4.

Claim 81

Dependent Claim 33 words

A malleable bone composition as claimed in claim 65 wherein said bone material includes cortical cancerous bone segments coarsely milled into irregular polyhedral shaped chips with edge dimensions up to about 5 mm.

Claim 82

Dependent Claim 37 words

A malleable bone composition as claimed in claim 65 wherein said bone powder contains growth factors such as transforming growth factor (TGF-beta), platelet derived growth factor (PDGF), osteopontin, fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1).

Claim 83

Dependent Claim 27 words

A malleable bone composition as claimed in claim 65 wherein said bone powder contains living cells such as blood cells, bone marrow cells and mesenchymal stem cells.

Claim 84

Dependent Claim 104 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a bone component particle size ranging from about 100 to about 800 microns to achieve a bone concentration ranging from about 25% to about 33% (w/w) in a gelatin and collagen ionic salt based phosphate buffer carrier, the combined weight of the gelatin and the collagen being about 10.0% by weight of the carrier.

Claim 85

Dependent Claim 104 words

A sterile malleable bone putty composition for application to a bone defect site to promote new bone growth at the site which comprises a new bone growth inducing compound of partially demineralized lyophilized allograft bone material with a calcium content greater than 4% and less than 8% dry weight and a bone component particle size ranging from about 100 to about 800 microns to achieve a bone concentration ranging from about 25% to about 33% (w/w) in a gelatin and collagen ionic salt based phosphate buffer carrier, the combined weight of the gelatin and the collagen being about 10.0% by weight of the carrier.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	2008-06-27	2008-12-27	2009-06-28	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	2012-06-27	2012-12-27	2013-06-28	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	2016-06-27	2016-12-27	2017-06-28	$7700.00	Window Closed / Lapsed

Malleable putty and flowable paste with allograft bone having residual calcium for filling bone defects

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