US6150328A
Expired / Lapsed
US
⏱️ 0.0 years remaining
BMP products
Assignee: Genetics Institute LLC
•
Filing: 1991-06-14
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Granted: 2000-11-21
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Expires: 2011-06-14
Abstract
Abstract Purified BMP-2 and BMP-4 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 Elizabeth A. Wang
- 👤 John M. Wozney
- 👤 Vicki A. Rosen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:34
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61L24/00; A61L24/04; A61L24/043; C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
187 words
A method for induction selected from the group consisting of the induction of bone formation, cartilage formation and the formation of the combination thereof in a patient comprising administering an amount of a purified BMP-2 protein effective for inducing said formation of bone, cartilage and the combination thereof said BMP-2 protein produced by the steps of: (a) culturing under suitable conditions a cell transformed with a DNA sequence selected from the group consisting of (i) a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 of SEQ ID NO:3 encoding a subunit of BMP-2; and (ii) DNA sequences comprising a nucleotide sequence which encodes a subunit having the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO: 4 said DNA sequence in operative association with an expression control sequence therefor (b) recovering and purifying from said culture medium a protein wherein each subunit comprises the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO: 4 said protein having induction activity selected from the group consisting of bone, cartilage and the combination thereof.
Claim 2
Independent Claim
187 words
A method for induction selected from the group consisting of the induction of bone formation, cartilage formation and the formation of the combination thereof in a patient comprising administering an amount of a purified BMP-2 protein effective for inducing said formation of bone, cartilage and the combination thereof said BMP-2 protein produced by the steps of: (a) culturing under suitable conditions a cell transformed with a DNA sequence selected from the group consisting of (i) a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 of SEQ ID NO:3 encoding a subunit of BMP-2; and (ii) DNA sequences comprising a nucleotide sequence which encodes a subunit having the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO: 4 said DNA sequence in operative association with an expression control sequence therefor (b) recovering and purifying from said culture medium a protein wherein each subunit comprises the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO: 4 said protein having induction activity selected from the group consisting of bone, cartilage and the combination thereof.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein said induction comprises the induction of bone formation.
Claim 4
Dependent Claim
14 words
The method of claim 1 wherein said induction comprises the induction of cartilage formation.
Claim 5
Dependent Claim
16 words
The method of claim 1 wherein said induction comprises the induction of bone and cartilage formation.
Claim 6
Dependent Claim
17 words
The method of claim 1 wherein said BMP-2 protein comprises amino acids #1-114 of SEQ ID NO:4.
Claim 7
Dependent Claim
10 words
The method of claim 1 wherein said cell is mammalian.
Claim 8
Dependent Claim
12 words
The method of claim 1 wherein said cell is a CHO cell.
Claim 9
Independent Claim
184 words
A method for induction selected from the group consisting of the induction of bone formation, cartilage formation and the formation of the combination thereof in a patient comprising administering an amount of a purified BMP-4 protein effective for inducing said formation of bone, cartilage and the combination thereof said BMP-4 protein produced by the steps of (a) culturing under suitable conditions a cell transformed with a DNA sequence selected from the group consisting of (i) a cDNA comprising the nucleotide sequence from nucleotide #403 to #1626 of SEQ ID NO: 5 encoding a subunit of BMP-4; and (ii) DNA sequences comprising a nucleotide sequence which encodes a subunit having the amino acid sequence from amino acid #19 to #116 of SEQ ID NO:6 said DNA sequence in operative association with an expression control sequence therefor (b) recovering and purifying from said culture medium a protein wherein each subunit comprises the 98 amino acid sequence from amino acid #19 to amino acid #116 of SEQ ID NO: 6 said protein having induction activity selected from the group consisting of bone, cartilage and the combination thereof.
Claim 10
Independent Claim
184 words
A method for induction selected from the group consisting of the induction of bone formation, cartilage formation and the formation of the combination thereof in a patient comprising administering an amount of a purified BMP-4 protein effective for inducing said formation of bone, cartilage and the combination thereof said BMP-4 protein produced by the steps of (a) culturing under suitable conditions a cell transformed with a DNA sequence selected from the group consisting of (i) a cDNA comprising the nucleotide sequence from nucleotide #403 to #1626 of SEQ ID NO: 5 encoding a subunit of BMP-4; and (ii) DNA sequences comprising a nucleotide sequence which encodes a subunit having the amino acid sequence from amino acid #19 to #116 of SEQ ID NO:6 said DNA sequence in operative association with an expression control sequence therefor (b) recovering and purifying from said culture medium a protein wherein each subunit comprises the 98 amino acid sequence from amino acid #19 to amino acid #116 of SEQ ID NO: 6 said protein having induction activity selected from the group consisting of bone, cartilage and the combination thereof.
Claim 11
Dependent Claim
14 words
The method of claim 8 wherein said induction comprises the induction of bone formation.
Claim 12
Dependent Claim
14 words
The method of claim 8 wherein said induction comprises the induction of cartilage formation.
Claim 13
Dependent Claim
16 words
The method of claim 8 wherein said induction comprises the induction of bone and cartilage formation.
Claim 14
Dependent Claim
18 words
The method of claim 8 wherein said BMP-4 protein comprises amino acids #1-#116 of SEQ ID NO: 6.
Claim 15
Dependent Claim
10 words
The method of claim 8 wherein said cell is mammalian.
Claim 16
Dependent Claim
12 words
The method of claim 8 wherein said cell is a CHO cell.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-11-21 | 2004-05-22 | 2004-11-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-11-21 | 2008-05-22 | 2008-11-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-11-21 | 2012-05-22 | 2012-11-21 | $7700.00 | Window Closed / Lapsed |