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US5554497A Expired / Lapsed US ⏱️ 0.0 years remaining

Cardioplegic solution for arresting an organ

Assignee: Carolinas Healthcare System
•
Filing: 1994-12-12
•
Granted: 1996-09-10
•
Expires: 2014-12-12
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Abstract

Abstract There is provided cardioplegic solutions for arresting an organ intended for transplantation and preservation solutions for perfusing and storing an organ while awaiting transplantation. The cardioplegic solutions include, per liter of solution, a balanced isotonic solution of sodium, potassium, calcium, and magnesium ions and bicarbonate in a physiologically acceptable amount, from about 0.5 mu M to about 2.0 mu M of an amiloride-containing compound; and water sufficient to make a liter of solution. The amiloride-containing compound may be amiloride, hexamethylene amiloride, dimethyl amiloride, ethyl isopropyl amiloride or methyl isobutyl amiloride. The preserving solutions are based on the isotonic solutions described and include other components such as EDTA, a small amount of adenosine, and at least one antioxidant. There is also provided a method for arresting an organ, storing an organ and transplanting an organ all at room temperature for up to at least 24 hours.

Inventors

  • 👤 Richard M. Raymond

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 19:49
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126

Claims Analysis

Total Claims: 34
Claim 1
Independent Claim 49 words
A cardioplegic solution for arresting a heart intended for transplantation comprising, per liter of solution: (a) a balanced isotonic solution comprising sodium, potassium, calcium, magnesium and bicarbonate ions in a physiologically acceptable amount; (b) from about 0.5 μM to about 2.0 μM of an amiloride-containing compound; and (c) water.
Claim 2
Dependent Claim 49 words
A cardioplegic solution for arresting a heart intended for transplantation comprising, per liter of solution: (a) a balanced isotonic solution comprising sodium, potassium, calcium, magnesium and bicarbonate ions in a physiologically acceptable amount; (b) from about 0.5 μM to about 2.0 μM of an amiloride-containing compound; and (c) water.
Claim 3
Dependent Claim 32 words
The cardioplegic solution according to claim 1, wherein said amiloride-containing compound is a compound selected from the group consisting of amiloride, hexamethylene amiloride, dimethyl amiloride, ethyl isopropyl amiloride and methyl isobutyl amiloride.
Claim 4
Dependent Claim 14 words
The cardioplegic solution according to claim 1, wherein said amiloride-containing compound is dimethyl amiloride.
Claim 5
Dependent Claim 19 words
The cardioplegic solution according to claim 1, further comprising from about 0.5 mM to about 1.5 mM of EDTA.
Claim 6
Dependent Claim 19 words
The cardioplegic solution according to claim 1, further comprising from about 5.0 μM to about 15 μM of adenosine.
Claim 7
Dependent Claim 19 words
The cardioptegic solution according to claim 1, further comprising from about 500 units to about 1500 units of heparin.
Claim 8
Dependent Claim 23 words
The cardioplegic solution according to claim 1, further comprising an antioxidant in an amount effective to inhibit the generation of oxygen-derived free radicals.
Claim 9
Dependent Claim 83 words
A cardioplegic solution for arresting a heart intended for transplantation comprising, per liter of solution: ______________________________________ NaCl 85 mM to 145 mM KCl 20 mM to 30 mM CaCl.sub.2 0.5 mM to 2.5 mM KH.sub.2 PO.sub.4 0.7 mM to 1.3 mM MgSO.sub.4 0.9 mM to 4.8 mM NaHCO.sub.3 15 mM to 35 mM Glucose 1.0 mM to 50 mM EDTA 0.5 mM to 1.5 mm Distilled, deionized water q.s. ______________________________________ and from about 0.5 μM to about 2.0 μM of an amiloride-containing compound.
Claim 10
Dependent Claim 83 words
A cardioplegic solution for arresting a heart intended for transplantation comprising, per liter of solution: ______________________________________ NaCl 85 mM to 145 mM KCl 20 mM to 30 mM CaCl.sub.2 0.5 mM to 2.5 mM KH.sub.2 PO.sub.4 0.7 mM to 1.3 mM MgSO.sub.4 0.9 mM to 4.8 mM NaHCO.sub.3 15 mM to 35 mM Glucose 1.0 mM to 50 mM EDTA 0.5 mM to 1.5 mm Distilled, deionized water q.s. ______________________________________ and from about 0.5 μM to about 2.0 μM of an amiloride-containing compound.
Claim 11
Dependent Claim 32 words
The cardioplegic solution according to claim 8, wherein said amiloride-containing compound is a compound selected from the group consisting of amiloride, hexamethylene amiloride, dimethyl amiloride, ethyl isopropyl amiloride and methyl isobutyl amiloride.
Claim 12
Dependent Claim 15 words
The cardioplegic solution according to claim 8, wherein said amiloride containing compound is dimethyl amiloride.
Claim 13
Dependent Claim 19 words
The cardioplegic solution according to claim 8, further comprising from about 5.0 μM to about 15 μM of adenosine.
Claim 14
Dependent Claim 19 words
The cardioplegic solution according to claim 8, further comprising from about 500 units to about 1500 units of heparin.
Claim 15
Dependent Claim 23 words
The cardioplegic solution according to claim 8, further comprising an antioxidant in an amount effective to inhibit the generation of oxygen-derived free radicals.
Claim 16
Dependent Claim 56 words
A cardioplegic solution for arresting a heart intended for transplantation comprising, per liter of solution: ______________________________________ NaCl 118 mM KCl 30 mM CaCl.sub.2 1.75 mM KH.sub.2 PO.sub.4 1.2 mM MgSO.sub.4 1.2 mM NaHCO.sub.3 25 mM Glucose 11 mM Adenosine 10 μM EDTA 1.0 mM Dimethyl amiloride 1.0 μM Heparin 1000 units Distilled, deionized water q.s. ______________________________________
Claim 17
Dependent Claim 56 words
A cardioplegic solution for arresting a heart intended for transplantation comprising, per liter of solution: ______________________________________ NaCl 118 mM KCl 30 mM CaCl.sub.2 1.75 mM KH.sub.2 PO.sub.4 1.2 mM MgSO.sub.4 1.2 mM NaHCO.sub.3 25 mM Glucose 11 mM Adenosine 10 μM EDTA 1.0 mM Dimethyl amiloride 1.0 μM Heparin 1000 units Distilled, deionized water q.s. ______________________________________
Claim 18
Independent Claim 72 words
A method for removing a heart from a mammal intended for implantation in a mammal requiring such implantation, said method comprising: arresting said heart prior to removal from a donor with a solution comprising, per liter of solution: (a) a balanced isotonic solution comprising sodium, potassium, calcium, magnesium, and bicarbonate ions in a physiologically acceptable amount; (b) from about 0.5 μM to about 2.0 μM of an amiloride-containing compound; and (c) water.
Claim 19
Independent Claim 72 words
A method for removing a heart from a mammal intended for implantation in a mammal requiring such implantation, said method comprising: arresting said heart prior to removal from a donor with a solution comprising, per liter of solution: (a) a balanced isotonic solution comprising sodium, potassium, calcium, magnesium, and bicarbonate ions in a physiologically acceptable amount; (b) from about 0.5 μM to about 2.0 μM of an amiloride-containing compound; and (c) water.
Claim 20
Dependent Claim 32 words
The method according to claim 15, in which said amiloride-containing compound is a compound selected from the group consisting of amiloride, hexamethylene amiloride, dimethyl amiloride, ethyl isopropyl amiloride and methyl isobutyl amiloride.
Claim 21
Dependent Claim 14 words
The method according to claim 15, in which said amiloride-containing compound is dimethyl amiloride.
Claim 22
Dependent Claim 21 words
The method according to claim 15, in which said solution further comprises from about 0.5 mM to about 1.5 mM EDTA.
Claim 23
Dependent Claim 22 words
The method according to claim 15, in which said solution further comprising from about 5.0 μM to about 15 μM of adenosine.
Claim 24
Dependent Claim 22 words
The method according to claim 15, in which said solution further comprising from about 500 units to about 1500 units of heparin.
Claim 25
Dependent Claim 28 words
The method according to claim 15, in which said solution further comprising at least one antioxidant in an amount effective to inhibit the generation of oxygen-derived free radicals.
Claim 26
Independent Claim 100 words
A method for removing the heart from a mammal intended for transplantation, said method comprising: arresting the heart prior to removal from a donor with a solution comprising, per liter of solution: ______________________________________ NaCl 85 mM to 145 mM KCl 3 mM to 30 mM CaCl.sub.2 0.5 mM to 2.5 mM KH.sub.2 PO.sub.4 0.7 mM to 1.3 mM MgSO.sub.4 0.9 mM to 4.8 mM NaHCO.sub.3 15 mM to 35 mM Glucose 1.0 mM to 50 mM EDTA 0.5 mM to 1.5 mm Distilled, deionized water q.s. ______________________________________ and from about 0.5 μM to about 2.0 μM of an amiloride-containing compound.
Claim 27
Independent Claim 100 words
A method for removing the heart from a mammal intended for transplantation, said method comprising: arresting the heart prior to removal from a donor with a solution comprising, per liter of solution: ______________________________________ NaCl 85 mM to 145 mM KCl 3 mM to 30 mM CaCl.sub.2 0.5 mM to 2.5 mM KH.sub.2 PO.sub.4 0.7 mM to 1.3 mM MgSO.sub.4 0.9 mM to 4.8 mM NaHCO.sub.3 15 mM to 35 mM Glucose 1.0 mM to 50 mM EDTA 0.5 mM to 1.5 mm Distilled, deionized water q.s. ______________________________________ and from about 0.5 μM to about 2.0 μM of an amiloride-containing compound.
Claim 28
Dependent Claim 31 words
The method according to claim 22, wherein said amiloride-containing compound is a compound selected from the group consisting of amiloride, hexamethylene amiloride, dimethyl amiloride, ethyl isopropyl amiloride and methyl isobutyl amiloride.
Claim 29
Dependent Claim 13 words
The method according to claim 22, wherein said amiloride-containing compound is dimethyl amiloride.
Claim 30
Dependent Claim 22 words
The method according to claim 22, in which said solution further comprising from about 5 μM to about 15 μM of adenosine.
Claim 31
Dependent Claim 22 words
The method according to claim 22, in which said solution further comprising from about 500 units to about 1500 units of heparin.
Claim 32
Dependent Claim 28 words
The method according to claim 22, in which said solution further comprising at least one antioxidant in an amount effective to inhibit the generation of oxygen-derived free radicals.
Claim 33
Independent Claim 73 words
A method for removing a heart from a mammal intended for implantation, said method comprising: arresting the heart prior to removal from a donor with a solution comprising, per liter of solution: ______________________________________ NaCl 118 mM KCl 30 mM CaCl.sub.2 1.75 mM KH.sub.2 PO.sub.4 1.2 mM MgSO.sub.4 1.2 mM NaHCO.sub.3 25 mM Glucose 11 mM Adenosine 10 μM EDTA 1.0 mM Dimethyl amiloride 1.0 μM Heparin 1000 units Distilled, deionized water q.s. ______________________________________
Claim 34
Independent Claim 73 words
A method for removing a heart from a mammal intended for implantation, said method comprising: arresting the heart prior to removal from a donor with a solution comprising, per liter of solution: ______________________________________ NaCl 118 mM KCl 30 mM CaCl.sub.2 1.75 mM KH.sub.2 PO.sub.4 1.2 mM MgSO.sub.4 1.2 mM NaHCO.sub.3 25 mM Glucose 11 mM Adenosine 10 μM EDTA 1.0 mM Dimethyl amiloride 1.0 μM Heparin 1000 units Distilled, deionized water q.s. ______________________________________

References Cited (Backward Citations)

US4798824A Cited
US4873230A Cited
US4879283A Cited
US4920044A Cited
US5145771A Cited
CA2089336A1 Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1999-09-10 2000-03-11 2000-09-10 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2003-09-10 2004-03-11 2004-09-10 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2007-09-10 2008-03-11 2008-09-10 $7700.00 Window Closed / Lapsed

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