Patent US2212481A successfully refreshed from Google Patents.
US5145771A
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Rinse solution for organs and tissues
Assignee: University of North Carolina at Chapel Hill
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Filing: 1990-10-10
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Granted: 1992-09-08
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Expires: 2010-10-10
Abstract
Abstract Disclosed is a rinse for preservation solution for organs and tissues selected for transplantation. The solution can be used at all temperatures from 0 DEG C. to normal body temperature and continues to be protective above 10 DEG C. Methods for preparing organs and tissues preserved in a preservation solution prior to transplant into a recipient are also disclosed.
Inventors
- 👤 John J. Lemasters
- 👤 Ronald G. Thurman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:43
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
100 words
A solution for the preservation and storage of organs and tissues, at temperatures of 0° C. to 37° C. intended for implantation in a patient requiring such implantation, and for rinsing other preservation and storage solution from an organ prior to implantation, said solution comprising, in one liter of solution: from about 0.12 to about 1.2 mM adenosine; sodium, potassium, calcium and magnesium ions in a physiologically acceptable amount; and water for injection sufficient to make a liter of solution; said solution having a pH of about 6.0 to 7.5, and a concentration of potassium of less than 6 MEQ/L.
Claim 2
Dependent Claim
100 words
A solution for the preservation and storage of organs and tissues, at temperatures of 0° C. to 37° C. intended for implantation in a patient requiring such implantation, and for rinsing other preservation and storage solution from an organ prior to implantation, said solution comprising, in one liter of solution: from about 0.12 to about 1.2 mM adenosine; sodium, potassium, calcium and magnesium ions in a physiologically acceptable amount; and water for injection sufficient to make a liter of solution; said solution having a pH of about 6.0 to 7.5, and a concentration of potassium of less than 6 MEQ/L.
Claim 3
Dependent Claim
10 words
The solution of claim 1 being substantially free of oxygen.
Claim 4
Dependent Claim
58 words
A solution according to claim 1, further comprising about 5% by weight of hydroxyethyl starch, said hydroxyethyl starch having a weight average molecular weight of from about 50,000 to about 1,000,000 daltons, a degree of substitution of from about 0.4 to about 0.7, and being substantially free of contaminants, including ethylene glycol, ethylene chlorohydrin, acetone and sodium chloride.
Claim 5
Dependent Claim
71 words
A solution for the rinsing of organs intended for implantation in a patient requiring such implantation prior to implantation and for the storage of such organs, said solution comprising: ______________________________________
NaCl 85 to 145 mM
KCl 3 to 6 mM
CaCl.sub.2 1.0 to 1.6 mM
KH.sub.2 PO.sub.4 0.7 to 1.3 mM
Adenosine 0.12 to 1.2 mM
Distilled deionized q.s.
water
______________________________________ and said solution having a pH of 6.0 to 7.4.
Claim 6
Dependent Claim
71 words
A solution for the rinsing of organs intended for implantation in a patient requiring such implantation prior to implantation and for the storage of such organs, said solution comprising: ______________________________________
NaCl 85 to 145 mM
KCl 3 to 6 mM
CaCl.sub.2 1.0 to 1.6 mM
KH.sub.2 PO.sub.4 0.7 to 1.3 mM
Adenosine 0.12 to 1.2 mM
Distilled deionized q.s.
water
______________________________________ and said solution having a pH of 6.0 to 7.4.
Claim 7
Independent Claim
90 words
A method for rinsing preservation or storage solution from organs or tissue intended for implantation in a patient requiring such implantation, said method comprising: rinsing said organ or tissue prior to implantation with a solution comprising in one liter of solution: from about 0.12 to 1.2 mM adenosine; sodium, potassium, calcium and magnesium ions in a physiologically acceptable amount; and water for injection sufficient to make a liter of solution; said solution having a pH of about 6.0 to 7.5, and a concentration of potassium of less than 6 MEQ/L.
Claim 8
Independent Claim
90 words
A method for rinsing preservation or storage solution from organs or tissue intended for implantation in a patient requiring such implantation, said method comprising: rinsing said organ or tissue prior to implantation with a solution comprising in one liter of solution: from about 0.12 to 1.2 mM adenosine; sodium, potassium, calcium and magnesium ions in a physiologically acceptable amount; and water for injection sufficient to make a liter of solution; said solution having a pH of about 6.0 to 7.5, and a concentration of potassium of less than 6 MEQ/L.
Claim 9
Independent Claim
95 words
A method for the preservation and storage at from about 12° C. to about 37° C. of organs or tissue intended for implantation in a patient requiring such implantation, said method comprising: contacting said organ or tissue with a solution comprising, in one liter of solution: from about 0.12 to 1.2 mM adenosine; sodium, potassium, calcium and magnesium ions in a physiologically acceptable amount; and water for injection sufficient to make a liter of solution; said solution having a pH of about 6.0 to 7.5, and a concentration of potassium of less than 6 MEQ/L.
Claim 10
Independent Claim
95 words
A method for the preservation and storage at from about 12° C. to about 37° C. of organs or tissue intended for implantation in a patient requiring such implantation, said method comprising: contacting said organ or tissue with a solution comprising, in one liter of solution: from about 0.12 to 1.2 mM adenosine; sodium, potassium, calcium and magnesium ions in a physiologically acceptable amount; and water for injection sufficient to make a liter of solution; said solution having a pH of about 6.0 to 7.5, and a concentration of potassium of less than 6 MEQ/L.
Claim 11
Dependent Claim
11 words
A solution according to claim 1 having a pH of 6.5.
Claim 12
Dependent Claim
14 words
A solution according to claim 1, further comprising from 0.05 to 5.0 mM allopurinol.
Claim 13
Dependent Claim
13 words
A solution according to claim 1, further comprising 0.5 to 10 mM glutathione.
Claim 14
Dependent Claim
13 words
A solution according to claim 1, further comprising 0.1 to 5.0 mM nicardipine.
Claim 15
Dependent Claim
13 words
A solution according to claim 1, further comprising 0.02 to 2.0 mM desferrioxamine.
Claim 16
Dependent Claim
11 words
A solution according to claim 4 being substantially free of oxygen.
Claim 17
Dependent Claim
11 words
A solution according to claim 4, having a pH of 6.5.
Claim 18
Dependent Claim
14 words
A solution according to claim 4, further comprising from 0.05 to 5.0 mM allopurinol.
Claim 19
Dependent Claim
13 words
A solution according to claim 4, further comprising 0.5 to 10 mM glutathione.
Claim 20
Dependent Claim
13 words
A solution according to claim 4, further comprising 0.1 to 5.0 mM nicardipine.
Claim 21
Dependent Claim
13 words
A solution according to claim 4, further comprising 0.02 to 2.0 mM desferrioxamine.
Claim 22
Dependent Claim
51 words
A method according to claim 5, said solution comprising: ______________________________________
NaCl 85 to 145 mM
KCl 3 to 6 mM
CaCl.sub.2 1.0 to 1.6 mM
KH.sub.2 PO.sub.4 0.7 to 1.3 mM
Adenosine 0.12 to 1.2 mM
Distilled deionized q.s.
water
______________________________________ and said solution having a pH of 6.0 to 7.4.
Claim 23
Dependent Claim
13 words
A solution according to claim 5, said solution being substantially free of oxygen.
Claim 24
Dependent Claim
13 words
A solution according to claim 5, said solution having a pH of 6.5.
Claim 25
Dependent Claim
16 words
A solution according to claim 5, said solution further comprising from 0.05 to 5.0 mM allopurinol.
Claim 26
Dependent Claim
15 words
A solution according to claim 5, said solution further comprising 0.5 to 10 mM glutathione.
Claim 27
Dependent Claim
15 words
A solution according to claim 5, said solution further comprising 0.1 to 5.0 mM nicardipine.
Claim 28
Dependent Claim
15 words
A solution according to claim 5, said solution further comprising 0.02 to 2.0 mM desferrioxamine.
Claim 29
Dependent Claim
51 words
A method according to claim 5, said solution comprising: ______________________________________
NaCl 85 to 145 mM
KCl 3 to 6 mM
CaCl.sub.2 1.0 to 1.6 mM
KH.sub.2 PO.sub.4 0.7 to 1.3 mM
Adenosine 0.12 to 1.2 mM
Distilled deionized q.s.
water
______________________________________ and said solution having a pH of 6.0 to 7.4.
Claim 30
Dependent Claim
13 words
A method according to claim 6, said solution being substantially free of oxygen.
Claim 31
Dependent Claim
13 words
A solution according to claim 6, said solution having a pH of 6.5.
Claim 32
Dependent Claim
16 words
A solution according to claim 6, said solution further comprising from 0.05 to 5.0 mM allopurinol.
Claim 33
Dependent Claim
15 words
A solution according to claim 6, said solution further comprising 0.5 to 10 mM glutathione.
Claim 34
Dependent Claim
15 words
A solution according to claim 6, said solution further comprising 0.1 to 5.0 mM nicardipine.
Claim 35
Dependent Claim
15 words
A solution according to claim 6, said solution further comprising 0.02 to 2.0 mM desferrioxamine.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1995-09-08 | 1996-03-09 | 1996-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-08 | 2000-03-09 | 2000-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-08 | 2004-03-09 | 2004-09-08 | $7700.00 | Window Closed / Lapsed |