US5326357A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reconstituted cartridge tissue
Assignee: Mount Sinai Hospital
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Filing: 1992-03-18
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Granted: 1994-07-05
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Expires: 2012-03-18
Abstract
Abstract The present invention relates to cartilage tissue reconstituted on a substrate; to a method for producing reconstituted cartilage tissue; and to cartilage tissue reconstituted in vitro from isolated chondrocytes cultured on a substrate.
Inventors
- 👤 Rita A. Kandel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:50
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3604; A61L27/3612; A61L27/3633; A61L27/3641; A61L27/3645; A61L27/365; A61L27/3654; A61P; A61P43/00; C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0655; A61F; A61F2/00; A61F2/02; A61F2/30; A61F2/30756; A61F2310/00; A61F2310/00005; A61F2310/00365; A61K; A61K35/00; A61K35/12; A61L2430/00; A61L2430/06; C12N2501/00; C12N2501/20; C12N2501/23; C12N2533/00; C12N2533/50; C12N2533/54
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
67 words
A biological material comprising cartilage tissue reconstituted on a substrate in vitro from isolated chondrocytes and characterized by a continuous layer of cartilage tissue, having a substantial extracellular matrix and comprising: a superficial zone wherein chondrocytes are flattened and arranged parallel to the substrate; mid and deep zones wherein the chondrocytes are spherical; and wherein the matrix in the superficial, mid and deep zones contains collagen fibres.
Claim 2
Dependent Claim
67 words
A biological material comprising cartilage tissue reconstituted on a substrate in vitro from isolated chondrocytes and characterized by a continuous layer of cartilage tissue, having a substantial extracellular matrix and comprising: a superficial zone wherein chondrocytes are flattened and arranged parallel to the substrate; mid and deep zones wherein the chondrocytes are spherical; and wherein the matrix in the superficial, mid and deep zones contains collagen fibres.
Claim 3
Dependent Claim
24 words
A biological material as claimed in claim 1 wherein the substrate comprises a porous tissue culture insert coated with at least one attachment factor.
Claim 4
Dependent Claim
14 words
A biological material as claimed in claim 2 wherein the insert is a MILLICELL®-CM.
Claim 5
Dependent Claim
18 words
A biological material as claimed in claim 2 wherein at least one of the attachment factors is collagen.
Claim 6
Dependent Claim
17 words
A biological material as claimed in claim 4 wherein the collagen is type I or II collagen.
Claim 7
Dependent Claim
16 words
A biological material as claimed in claim 1, wherein the collagen is predominantly type II collagen.
Claim 8
Dependent Claim
29 words
A biological material as claimed in claim 1, wherein the cartilage tissue contains proteoglycans containing galactosamine and glucosamine having a ratio of galactosamine to glucosamine of 4.5 to 6.5.
Claim 9
Dependent Claim
15 words
A biological material as claimed in claim 1, further containing sulphated proteoglycans within the matrix.
Claim 10
Dependent Claim
24 words
A biological material as claimed in claim 8, wherein the sulphated proteoglycans increase in abundance from the superficial to the mid and deep zones.
Claim 11
Independent Claim
118 words
A method for producing a biological material comprising reconstituted cartilage tissue comprising: isolating chondrocytes from articular cartilage tissue; forming a monolayer of chondrocytes on a substrate which is selected to permit growth media to be applied above and below the substrate; culturing the chondrocytes on the substrate in growth media which is applied above and below the chondrocytes on the substrate to produce a biological material characterized by a continuous layer of cartilage tissue having a substantial extracellular matrix and comprising: a superficial zone wherein chondrocytes are flattened and arranged parallel to the substrate; and mid and deep zones wherein the chondrocytes are spherical; and wherein the matrix in the superficial, mid and deep zones contains collagen fibres.
Claim 12
Independent Claim
118 words
A method for producing a biological material comprising reconstituted cartilage tissue comprising: isolating chondrocytes from articular cartilage tissue; forming a monolayer of chondrocytes on a substrate which is selected to permit growth media to be applied above and below the substrate; culturing the chondrocytes on the substrate in growth media which is applied above and below the chondrocytes on the substrate to produce a biological material characterized by a continuous layer of cartilage tissue having a substantial extracellular matrix and comprising: a superficial zone wherein chondrocytes are flattened and arranged parallel to the substrate; and mid and deep zones wherein the chondrocytes are spherical; and wherein the matrix in the superficial, mid and deep zones contains collagen fibres.
Claim 13
Dependent Claim
17 words
A method as claimed in claim 10 wherein the chondrocytes are isolated from human articular cartilage tissue.
Claim 14
Dependent Claim
16 words
A method as claimed in claim 10 wherein the chondrocytes are isolated from bovine articular cartilage.
Claim 15
Dependent Claim
17 words
A method as claimed in claim 12, wherein the chondrocytes are isolated by sequential enzyme digestion techniques.
Claim 16
Dependent Claim
14 words
A method as claimed in claim 10 wherein the isolated chondrocytes are mesenchymal cells.
Claim 17
Dependent Claim
23 words
A method as claimed in claim 10 wherein the substrate is a porous tissue culture insert coated with at least one attachment factor.
Claim 18
Dependent Claim
15 words
A method as claimed in claim 15 wherein the porous tissue culture insert is MILLICELL®-CM.
Claim 19
Dependent Claim
48 words
A method as claimed in claim 15 wherein the attachment factors are selected from the group consisting of type I collagen, type II collagen, type IV collagen, fibronectin, gelatin, laminin, polylysine, vitronectin, cytotactin, echinonectin, entactin, tenascin, thrombospondin, uvomorulin, biglycan, chondroitin sulfate, decorin, dermatan sulfate, heparin, and hyaluronic acid.
Claim 20
Dependent Claim
19 words
A method as claimed in claim 15, wherein at least one of the attachment factors is type I collagen.
Claim 21
Dependent Claim
24 words
A method as claimed in claim 10, wherein the chondrocytes are cultured at a cell density of at least 0.1×10 6 /cm 2 .
Claim 22
Dependent Claim
24 words
A method as claimed in claim 10, wherein the chondrocytes are cultured at a cell density of 1.0 to 7×10 6 /cm 2 .
Claim 23
Dependent Claim
15 words
A method as claimed in claim 10, wherein the growth media is Ham's F12 medium.
Claim 24
Dependent Claim
17 words
A method as claimed in claim 10 wherein the chondrocytes are cultured for at least 14 days.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-07-04 | 1998-01-03 | 1998-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-07-04 | 2002-01-03 | 2002-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-07-04 | 2006-01-03 | 2006-07-05 | $7700.00 | Window Closed / Lapsed |