US4356261A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Anti-invasion factor containing cultures
Assignee: Rush Presbyterian St Lukes Medical Center
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Filing: 1980-04-22
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Granted: 1982-10-26
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Expires: 2000-04-22
Abstract
Abstract Chondrocytes are cultured to produce cartilage tissue from which an anti-invasion factor (AIF) may be recovered. Mammalian cartilage providing at least about 60% viable chondrocytes is enzymatically digested to denude the cells of their extracellular matrix. The denuded cells are plated at high density and cultured to produce cartilage tissue.
Inventors
- 👤 Klaus E. Kuettner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 20:59
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0655; A; A61; A61K; A61K35/00; A61K35/12; A61K35/32; A61L; A61L27/00; A61L27/36; A61L27/38; C12N2500/00; C12N2500/30; C12N2500/38
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
88 words
A method for culturing chondrocytes to produce cartilage tissue comprising: selecting mammalian cartilage tissue providing viable chondrocytes, preparing shavings from the cartilage tissue, digesting said shavings to remove the extracellular matrix to provide denuded cartilage cells, plating said denuded cells in a roller bottle in sufficient density to provide at least a monolayer of cells so that said cells are in surface contact with each other, and providing a growth medium and rotating said roller bottle to grow said cells and produce a culture with an extracellular matrix.
Claim 2
Independent Claim
88 words
A method for culturing chondrocytes to produce cartilage tissue comprising: selecting mammalian cartilage tissue providing viable chondrocytes, preparing shavings from the cartilage tissue, digesting said shavings to remove the extracellular matrix to provide denuded cartilage cells, plating said denuded cells in a roller bottle in sufficient density to provide at least a monolayer of cells so that said cells are in surface contact with each other, and providing a growth medium and rotating said roller bottle to grow said cells and produce a culture with an extracellular matrix.
Claim 3
Independent Claim
94 words
A method for producing anti-invasion factor comprising the steps of: selecing mammalian cartilage tissue providing viable chondrocytes; preparing shavings from the cartilage tissue; digesting said shavings to remove the extracellular matrix to provide denuded cartilage cells, plating said denuded cells in a roller bottle in sufficient density to provide at least a monolayer of cells so that said cells are in surface contact with each other, providing a growth medium and rotating said roller bottle to grow said cells and produce a culture with an extracellular matrix, and extracting anti-invasion factor from said culture.
Claim 4
Independent Claim
94 words
A method for producing anti-invasion factor comprising the steps of: selecing mammalian cartilage tissue providing viable chondrocytes; preparing shavings from the cartilage tissue; digesting said shavings to remove the extracellular matrix to provide denuded cartilage cells, plating said denuded cells in a roller bottle in sufficient density to provide at least a monolayer of cells so that said cells are in surface contact with each other, providing a growth medium and rotating said roller bottle to grow said cells and produce a culture with an extracellular matrix, and extracting anti-invasion factor from said culture.
Claim 5
Dependent Claim
30 words
A method according to claim 1 or claim 2 wherein at least about 2×10 5 of said denuded cells are plated per cm 2 surface area of said roller bottle.
Claim 6
Dependent Claim
18 words
A method according to claim 2 wherein anti-invasion factor is recovered from the growth medium of said culture.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-10-25 | 1986-04-26 | 1986-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-10-25 | 1990-04-26 | 1990-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-10-25 | 1994-04-26 | 1994-10-26 | $7700.00 | Window Closed / Lapsed |