US4757017A
Expired / Lapsed
US
⏱️ 0.0 years remaining
In vitro cell culture system
Assignee: MCW RESEARCH FOUNDATION Inc A CORP OF WI
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Filing: 1984-09-14
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Granted: 1988-07-12
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Expires: 2004-09-14
Abstract
Abstract In vitro cell culture systems for anchorage-dependent mammalian cells using solid substrates of mitogenic calcium compounds, such as hydroxyapatite and tricalcium phosphate forms of calcium phosphate and calcium carbonate. The calcium solid substrates can be in the form of granules with a particle size of at least 0.050 mm. or in the form of solid bodies and may be either porous or non-porous. Unique features of cells cultured in the stated in vitro cell culture systems include the growth of cells in layers many cells thick, growth of cells that maintain their phenotype and the ability to culture cells for extended periods of time.
Inventors
- 👤 Herman S. Cheung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:20
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/10; C12N2533/18
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
98 words
In a method for in vitro culture of anchorage-dependent mammalian cells on the surface of a solid substrate immersed in a nutrient growth medium, the improvement comprising: providing a solid substrate consisting of a calcium compound which is mitogenic and non-toxic to the anchorage dependent mammalian cells and of a size sufficient to provide support; seeding the cells on the solid substrate; adding nutrient growth medium to cover the solid substrate and the cells; and growing the cells on the solid substrate for a time sufficient to grow multi-cell thick layers of the cells on the solid substrate.
Claim 2
Dependent Claim
98 words
In a method for in vitro culture of anchorage-dependent mammalian cells on the surface of a solid substrate immersed in a nutrient growth medium, the improvement comprising: providing a solid substrate consisting of a calcium compound which is mitogenic and non-toxic to the anchorage dependent mammalian cells and of a size sufficient to provide support; seeding the cells on the solid substrate; adding nutrient growth medium to cover the solid substrate and the cells; and growing the cells on the solid substrate for a time sufficient to grow multi-cell thick layers of the cells on the solid substrate.
Claim 3
Dependent Claim
37 words
An in vitro cell culture method according to claim 1, including: providing the solid substrate in the form of granules of the calcium compound having a particle size of at least 0.05 millimeters in their largest dimension.
Claim 4
Dependent Claim
29 words
An in vitro cell culture method according to claim 2, wherein: the granules of the calcium compound have a particle size in the range of 0.050 to 1.0 millimeters.
Claim 5
Dependent Claim
26 words
An in vitro cell culture method according to claim, 1, including: providing the solid substrate in the form of a solid body of the calcium compound.
Claim 6
Dependent Claim
24 words
An in vitro cell culture method according to claim 4, wherein: the solid body is formed of sintered porous granules of the calcium compound.
Claim 7
Dependent Claim
32 words
An in vitro cell culture method according to claim 1, 2, 3, 4, or 5 wherein: the calcium compound is the hydroxyapatite or tricalcium phosphate form of calcium phosphate or calcium carbonate.
Claim 8
Dependent Claim
34 words
An in vitro cell culture method according to claim 2 or 3 including: growing the cells on the solid substrate for a period of time in the range of 4 weeks to 13 months.
References Cited (Backward Citations)
US3717551A
Cited
WO1983004177A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-07-12 | 1992-01-11 | 1992-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-07-12 | 1996-01-11 | 1996-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-07-12 | 2000-01-11 | 2000-07-12 | $7700.00 | Window Closed / Lapsed |