Patent US5106743A successfully refreshed from Google Patents.
US5106743A
Expired / Lapsed
US
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Hydrogels capable of supporting cell growth
Assignee: Boston University
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Filing: 1985-06-18
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Granted: 1992-04-21
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Expires: 2005-06-18
Abstract
Abstract A method of growing anchorage-dependent cells is disclosed in which a substrate comprising a hydrogel is employed. The hydrogel is formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and includes a macromolecule capable of supporting cell growth.
Inventors
- 👤 Carl Franzblau
- 👤 Barbara A. Faris
- 👤 Linda Civerchia-Perez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:57
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/50
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
56 words
A hydrogel comprising an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer, said crosslinked polymer having been formed by polymerization of a hydrophilic monomer in said aqueous solution, said aqueous solution additionally incorporating a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 2
Dependent Claim
56 words
A hydrogel comprising an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer, said crosslinked polymer having been formed by polymerization of a hydrophilic monomer in said aqueous solution, said aqueous solution additionally incorporating a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 3
Dependent Claim
11 words
A hydrogel of claim 1 wherein said macromolecule comprises a protein.
Claim 4
Dependent Claim
10 words
A hydrogel of claim 2 wherein said protein comprises collagen.
Claim 5
Dependent Claim
12 words
A hydrogel of claim 3 wherein said hydrophilic monomer comprises hydroxyethyl methacrylate.
Claim 6
Dependent Claim
47 words
A hydrogel formed by polymerizing a hydrophilic monomer in an aqueous solution to form a crosslinked polymer which gels said aqueous solution, the aqueous solution additionally containing a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 7
Dependent Claim
47 words
A hydrogel formed by polymerizing a hydrophilic monomer in an aqueous solution to form a crosslinked polymer which gels said aqueous solution, the aqueous solution additionally containing a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 8
Dependent Claim
10 words
A hydrogel of claim 5 wherein said macromolecule comprises collagen.
Claim 9
Dependent Claim
12 words
A hydrogel of claim 6 wherein said hydrophilic monomer comprises hydroxyethyl methacrylate.
Claim 10
Dependent Claim
101 words
A hydrogel capable of supporting growth of anchorage dependent mammalian cells, produced by: a. forming an aqueous solution containing: (i) a hydrophilic monomer in an amount which will, upon polymerization and crosslinking, produce a three-dimensional polymer network sufficient to gel said aqueous solution thereby forming a hydrogel; and additionally, (ii) a macromolecule capable of supporting cell growth, said macromolecule being present in an amount sufficient to enable said hydrogel to support growth of anchorage-dependent mammalian cells; and b. polymerizing and crosslinking said hydrophilic monomer to thereby form a hydrogel which integrates the macromolecule and which supports growth of anchorage-dependent mammalian cells.
Claim 11
Dependent Claim
101 words
A hydrogel capable of supporting growth of anchorage dependent mammalian cells, produced by: a. forming an aqueous solution containing: (i) a hydrophilic monomer in an amount which will, upon polymerization and crosslinking, produce a three-dimensional polymer network sufficient to gel said aqueous solution thereby forming a hydrogel; and additionally, (ii) a macromolecule capable of supporting cell growth, said macromolecule being present in an amount sufficient to enable said hydrogel to support growth of anchorage-dependent mammalian cells; and b. polymerizing and crosslinking said hydrophilic monomer to thereby form a hydrogel which integrates the macromolecule and which supports growth of anchorage-dependent mammalian cells.
Claim 12
Dependent Claim
11 words
A hydrogel of claim 8 wherein said macromolecule comprises water-soluble collagen.
Claim 13
Dependent Claim
12 words
A hydrogel of claim 9 wherein said hydrophilic monomer comprises hydroxyethyl methacrylate.
Claim 14
Dependent Claim
8 words
A hydrogel of claim 8 which is transparent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-04-21 | 1995-10-21 | 1996-04-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-04-21 | 1999-10-21 | 2000-04-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-04-21 | 2003-10-21 | 2004-04-21 | $7700.00 | Window Closed / Lapsed |