US4061466A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biologically active composition and the use thereof
Assignee: Individual
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Filing: 1975-10-14
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Granted: 1977-12-06
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Expires: 1995-10-14
Abstract
Abstract Immobilized biologically active substances comprising microporous, spherical particles in gel form and in the form of a three-dimensional network. These particles contain the biologically active substance entrapped in the meshes of the network. The particles have sizes below 10 μm and the average diameter of the particles is in the range of from 0.5 to 4 μm, preferably 1 μm. The biologically active substance is capable of exerting its biological activity against substances which cannot penetrate into the meshes of the network of the particles. Preferably, the particles are gel grains obtained by copolymerization of acrylic compounds with cross-linkers. The immobilized biologically active substances are used for labelling or separation of cells, cell fragments, viruses or tissue structures having specific receptors or of substances having specific surface structures.
Inventors
- 👤 Ingvar Gosta Holger Sjoholm
- 👤 Nils Roger Lindmark
- 👤 Bo Magnus Ekman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:45
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; A; A61; A61K; A61K9/00; A61K9/14; A61K9/16; A61K9/1605; A61K9/1629; A61K9/1635; A61K9/167; B; B01; B01J; B01J13/00; B01J13/02; B01J13/06; B01J13/14; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/549; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/816; Y10S436/828; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/815; Y10S530/817
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
51 words
A biologically active composition comprising microporous, spherical particles in gel form, said particles having a three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4μm and a biologically active macromolecular substance immobilized within the meshes of said polymeric network but retainining its biological activity.
Claim 2
Dependent Claim
51 words
A biologically active composition comprising microporous, spherical particles in gel form, said particles having a three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4μm and a biologically active macromolecular substance immobilized within the meshes of said polymeric network but retainining its biological activity.
Claim 3
Dependent Claim
29 words
A biologically active composition according to claim 1 wherein the spherical particles consist of a copolymer of a low-molecular acrylic compound or of 1-vinyl-2-pyrrolidinone with a low-molecular divinyl compound.
Claim 4
Dependent Claim
71 words
A biologically active composition according to claim 2 wherein the spherical particles consist of a copolymer of an acrylamide of the formula ##STR2## wherein R 1 is hydrogen or methyl and R 2 is OH or --NHR 3 , wherein R 3 is hydrogen or a straight or branched alkyl group of 1-6 carbon atoms, which alkyl group is unsubstituted or substituted with a hydroxyl or an oxo group, with N,N'-methylene-bis-acrylamide.
Claim 5
Dependent Claim
91 words
A process for carrying out a bioassay for quantitatively determining a particular substance in a biological sample comprising adding to said sample a reagent comprising of a biologically active composition comprising a. microporous, spherical particles in gel form, said particles having a three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4 μm and b. a biologically active macromolecular substance immobilized within the meshes of said polymeric network but retaining its biological activity, said biologically active macromolecular substance being specific to said particular substance.
Claim 6
Dependent Claim
91 words
A process for carrying out a bioassay for quantitatively determining a particular substance in a biological sample comprising adding to said sample a reagent comprising of a biologically active composition comprising a. microporous, spherical particles in gel form, said particles having a three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4 μm and b. a biologically active macromolecular substance immobilized within the meshes of said polymeric network but retaining its biological activity, said biologically active macromolecular substance being specific to said particular substance.
Claim 7
Dependent Claim
13 words
Process according to claim 4 wherein the average particle diameter is 1 μm.
Claim 8
Dependent Claim
32 words
Process according to claim 4 wherein the biologically active macromolecular substance is labelled with an atom or group capable of emitting radiation or with an enzyme or a group producing a color.
Claim 9
Dependent Claim
110 words
A process for labelling a biological member selected from the group consisting of cells, cell fragments, viruses, tissue structures having specific receptors and substances having specific surface conformations comprising contacting said member with a reagent comprising a biologically active composition comprising a. microporous, spherical particles in gel form, said particles having three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4 μm and b. a biologically active macromolecular substance immobilized within the meshes of said polymeric network but retainings its biological activity, said biologically active macromolecular substance being specific to said member and being labelled with a radioactive atom, an enzyme or fluorophore.
Claim 10
Dependent Claim
110 words
A process for labelling a biological member selected from the group consisting of cells, cell fragments, viruses, tissue structures having specific receptors and substances having specific surface conformations comprising contacting said member with a reagent comprising a biologically active composition comprising a. microporous, spherical particles in gel form, said particles having three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4 μm and b. a biologically active macromolecular substance immobilized within the meshes of said polymeric network but retainings its biological activity, said biologically active macromolecular substance being specific to said member and being labelled with a radioactive atom, an enzyme or fluorophore.
Claim 11
Dependent Claim
13 words
Process according to claim 7 wherein the average particle diameter is 1 μm.
Claim 12
Independent Claim
49 words
A method of separating a biological member selected from the group consisting of cells, cell fragments, viruses, tissue structures having specific receptors and substances having specific surface conformtions from a biological sample containing said member comprising mixing said biological sample with a reagent comprising a biologically active composition comprising
Claim 13
Independent Claim
49 words
A method of separating a biological member selected from the group consisting of cells, cell fragments, viruses, tissue structures having specific receptors and substances having specific surface conformtions from a biological sample containing said member comprising mixing said biological sample with a reagent comprising a biologically active composition comprising
Claim 14
Dependent Claim
71 words
a. microporous, spherical particles in gel form, said particles having a three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4 μm and b. a biologically active macromoleeular substance immobilized within the meshes of said polymeric network but retaining its biological activity, said biologically active macromolecular substance being specific to said member and separating the resulting conjugate of said member-said reagent by physical means.
Claim 15
Dependent Claim
71 words
a. microporous, spherical particles in gel form, said particles having a three-dimensional polymeric network, diameters less than 10 μm and an average diameter of from 0.5 to 4 μm and b. a biologically active macromoleeular substance immobilized within the meshes of said polymeric network but retaining its biological activity, said biologically active macromolecular substance being specific to said member and separating the resulting conjugate of said member-said reagent by physical means.
Claim 16
Dependent Claim
13 words
Method according to claim 9, wherein the average particle diameter is 1 μm.
Claim 17
Dependent Claim
32 words
Method according to claim 9 wherein the biologically active macromolecular substance is labelled with an atom or group capable of emitting radiation or with an enzyme or a group producing a color.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-05 | 1981-06-06 | 1981-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-05 | 1985-06-06 | 1985-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-05 | 1989-06-06 | 1989-12-06 | $7700.00 | Window Closed / Lapsed |