US5053453A
Expired / Lapsed
US
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Thromboresistant materials and methods for making same
Assignee: Baxter International Inc
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Filing: 1991-01-15
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Granted: 1991-10-01
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Expires: 2011-01-15
Abstract
Abstract Thromboresistant materials are disclosed comprising hirudin or hirudin derivatives covalently linked to support materials such that the resultant composition has substantially the same biological activity as hirudin. Methods for making such compositions are also disclosed.
Inventors
- 👤 Cecilia S. L. Ku
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:27
CPC Classifications
C; C07; C07K; C07K17/00; C07K17/02; C07K17/06; A; A61; A61K; A61K35/00; A61K35/56; A61K35/62; A61K47/00; A61K47/50; A61K47/51; A61K47/52; A61K47/56; A61K47/58; A61K47/61; A61K47/62; A61L; A61L33/00; A61L33/06; A61L33/12; A61L33/128; C07K14/00; C07K14/81; C07K14/815; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
36 words
A thromboresistant material comprising: a protein having an amino acid sequence sufficiently complementary to the hirudin binding sites of thrombin so as to have antithrombotic activity; covalently bound through a linking group to a support material.
Claim 2
Dependent Claim
36 words
A thromboresistant material comprising: a protein having an amino acid sequence sufficiently complementary to the hirudin binding sites of thrombin so as to have antithrombotic activity; covalently bound through a linking group to a support material.
Claim 3
Dependent Claim
13 words
The thromboresistant material of claim 1 wherein the support material is a polymer.
Claim 4
Dependent Claim
21 words
The thromboresistant material of claim 2 wherein the polymer is a naturally occurring polymer, genetically-derived polymer or synthetic polymer or copolymer.
Claim 5
Dependent Claim
30 words
The thromboresistant material of claim 2 wherein the polymer is selected from the group consisting of polyvinylchloride, polyethylene, polypropylene, polyacrylonitrile, poly(hydroxyethyl methacrylate), polytetrafluoroethylene, polyethylene terephthalate, polyamides, polyurethanes, polymethoxysiloxane and polysaccharides.
Claim 6
Dependent Claim
50 words
The thromboresistant material of claim 2 wherein the polymer is chosen from the group consisting of polyvinyl alcohol copolymers, glass, silica, polyhydroxyethyl methacrylate, maleic anhydride copolymers, carboxymethyl cellulose, modified silica gel, poly-p-amino styrene, polyethyleneimines, polymers treated with glutaraldehyde, polysaccharides treated with periodate, acetylmercaptosuccinic acid modified polymers, fluoropolymers, polyacrylonitrile and silanes.
Claim 7
Dependent Claim
16 words
The thromboresistant material of claim 1 wherein the support material is a membrane, tissue or organ.
Claim 8
Dependent Claim
13 words
The thromboresistant material of claim 1 wherein the support material is a metal.
Claim 9
Dependent Claim
13 words
The thromboresistant material of claim 1 wherein the support material is a ceramic.
Claim 10
Dependent Claim
13 words
The thromboresistant material of claim 1 wherein the support material is a glass.
Claim 11
Dependent Claim
26 words
The thromboresistant material of claim 1 wherein the linking group is selected from the group consisting of bifunctional protein crosslinking reagents, polypeptides, proteins, and multifunctional polymers.
Claim 12
Dependent Claim
24 words
The thromboresistant material of claim 1 wherein the protein is coupled to the support material by an amino acid residue in the N-terminal region.
Claim 13
Dependent Claim
19 words
The thromboresistant material of claim 1 wherein the protein is coupled to the support material by the C-terminal residue.
Claim 14
Independent Claim
39 words
A method for producing a thromboresistant material having substantially the same biological activity as hirudin comprising coupling a protein having substantially the same biological activity as hirudin through a linking group to a support material having active functional groups.
Claim 15
Independent Claim
39 words
A method for producing a thromboresistant material having substantially the same biological activity as hirudin comprising coupling a protein having substantially the same biological activity as hirudin through a linking group to a support material having active functional groups.
Claim 16
Dependent Claim
12 words
The method of claim 13 wherein the support material is a polymer.
Claim 17
Dependent Claim
21 words
The method of claim 14 wherein the polymer is a naturally occurring polymer, genetically derived polymer or synthetic polymer or copolymer.
Claim 18
Dependent Claim
29 words
The method of claim 14 wherein the polymer is selected from the group consisting of polyvinylchloride, polyethylene, polypropylene, polyacrylonitrile, poly(hydroxyethyl methacrylate), polytetrafluoroethylene, polyethylene terephthalate, polyamides, polyurethanes, polymethoxysiloxane and polysaccharides.
Claim 19
Dependent Claim
49 words
The method of claim 14 wherein the polymer is chosen from the group consisting of polyvinyl alcohol copolymers, glass, silica, polyhydroxyethyl methacrylate, maleic anhydride copolymers, carboxymethyl cellulose, modified silica gel, poly-p-amino styrene, polyethyleneimines, polymers treated with glutaraldehyde, polysaccharides treated with periodate, acetylmercaptosuccinic acid modified polymers, fluoropolymers, polyacrylonitrile and silanes.
Claim 20
Dependent Claim
15 words
The method of claim 13 wherein the support material is a membrane, tissue or organ.
Claim 21
Dependent Claim
12 words
The method of claim 13 wherein the support material is a metal.
Claim 22
Dependent Claim
12 words
The method of claim 13 wherein the support material is a ceramic.
Claim 23
Dependent Claim
12 words
The method of claim 13 wherein the support material is a glass.
Claim 24
Dependent Claim
46 words
The method of claim 13 wherein the linking group is first covalently bound to the protein and then the resulting protein-linking group is covalently bound by a functional group on the linking group end of the protein-linking group to the functional group of the support material.
Claim 25
Dependent Claim
48 words
The method of claim 13 wherein the linking group is first covalently bound to the functional group of the support material and then the resulting support material-linking group is covalently bound by a functional group on the linking group end of the support material-linking group to the protein.
Claim 26
Dependent Claim
25 words
The method of claim 22 wherein the linking group is selected from the group consisting of bifunctional protein crosslinking reagents, polypeptides, proteins, and multifunctional polymers.
Claim 27
Dependent Claim
23 words
The method of claim 13 wherein the protein is coupled to the support material by an amino acid residue in the N-terminal region.
Claim 28
Dependent Claim
18 words
The method of claim 13 wherein the protein is coupled to the support material by the C-terminal residue.
Claim 29
Dependent Claim
26 words
The method of claim 13 further comprising protecting active sites of in the thrombin binding region of the protein prior to coupling to the support material.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1994-09-30 | 1995-04-01 | 1995-10-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-30 | 1999-04-01 | 1999-10-01 | $3760.00 | Window Closed / Lapsed |
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