US4746731A
Expired / Lapsed
US
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Isolated tissue protein PP18
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1985-05-17
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Granted: 1988-05-24
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Expires: 2005-05-17
Abstract
Abstract A protein PP18 is isolated having the following characteristics: (a) an electrophoretic mobility in the region of that of beta 1-globulins; (b) an isoelectric point between 5.6 and 6.2; (c) a sedimentation coefficient s20,w of 5.0+/-0.2 S; (d) a molecular weight determined in an ultracentrifuge of 82,300+/-5,600; (e) a carbohydrate fraction of 2.3+/-1.3 g/100 g (mannose 0.15+/-0.1, xylose 0.5+/-0.5, galactose 0.5+/-0.2, glucose 0.2+/-0.1, N-acetylglucosamine 0.7+/-0.2, and N-acetylneuraminic acid 0.25+/-0.2, each g/100 g). Also described is the amino acid composition of the protein and a process for its isolation. Antiserum is prepared and used in an immunoassay to detect the protein.
Inventors
- 👤 Hans Bohn
- 👤 Wilhelm Winckler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:04
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4715; C07K16/00; C07K16/18; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/689; Y; Y10; Y10S; Y10S436/00; Y10S436/811
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
87 words
An isolated, concentrated tissue protein, PP 18 , obtained by fractionating an extract of human organs, said tissue protein having: (a) an electrophoretic mobility in the region of that of β 1 -globulins; (b) an isoelectric point between 5.6 and 6.2; (c) a sedimentation coefficient s 20 ,w of 5.0±0.2 S; (d) a molecular weight determined in an ultracentrifuge of 82,300±5,600; and (e) a carbohydrate fraction of 2.3±1.3 g/100 g (mannose 0.15±0.1, xylose 0.5±0.5, galactose 0.5±0.2, glucose 0.2±0.1, N-acetylgucosamine 0.7±0.2, and N-acetylneuraminic acid 0.25±0.2, each g/100 g).
Claim 2
Dependent Claim
87 words
An isolated, concentrated tissue protein, PP 18 , obtained by fractionating an extract of human organs, said tissue protein having: (a) an electrophoretic mobility in the region of that of β 1 -globulins; (b) an isoelectric point between 5.6 and 6.2; (c) a sedimentation coefficient s 20 ,w of 5.0±0.2 S; (d) a molecular weight determined in an ultracentrifuge of 82,300±5,600; and (e) a carbohydrate fraction of 2.3±1.3 g/100 g (mannose 0.15±0.1, xylose 0.5±0.5, galactose 0.5±0.2, glucose 0.2±0.1, N-acetylgucosamine 0.7±0.2, and N-acetylneuraminic acid 0.25±0.2, each g/100 g).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-05-24 | 1991-11-23 | 1992-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-24 | 1995-11-23 | 1996-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-24 | 1999-11-23 | 2000-05-24 | $7700.00 | Window Closed / Lapsed |