Patent US4721096A successfully refreshed from Google Patents.
US4721096A
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Process for replicating bone marrow in vitro and using the same
Assignee: Advanced BioHealing Inc
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Filing: 1987-04-03
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Granted: 1988-01-26
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Expires: 2007-04-03
Abstract
Abstract According to the present invention there is provided a process for treating a person whose bone marrow has been destroyed or lost its functional ability. The process includes the steps of obtaining bone marrow from a donor, cryropreserving the marrow, replicating the bone marrow cells in vitro, and then infusing the replicated bone marrow cells into a person whose bone marrow has been destroyed or functionally compromised by disease or the treatment of disease. The person receiving the replicated bone marrow infusion may be the donor or another person. There is also disclosed a process for replicating bone marrow in vitro.
Inventors
- 👤 Brian A. Naughton
- 👤 Gail K. Naughton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:18
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0669; A; A61; A61K; A61K35/00; A61K35/12; A61K35/28; A61L; A61L27/00; A61L27/50; A61L27/60; A61K2035/124; C12N2500/00; C12N2500/30; C12N2500/34; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/30; C12N2501/33; C12N2501/335; C12N2501/38; C12N2501/39; C12N2502/00; C12N2502/13; C12N2502/1323; C12N2510/00; C12N2533/00; C12N2533/30; C12N2533/40; C12N2533/70
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
77 words
A process for treating a person whose bone marrow has been destroyed or lost its functional ability, said process comprising the steps of: I. obtaining a bone marrow sample from a donor; and then II. replicating the bone marrow sample in vitro to produce a replicated bone marrow containing hematopoietic stem cells having marrow repopulating activity; and then III. infusing the replicated bone marrow containing hematopoietic stem cells into the person to restore hematopoiesis in the person.
Claim 2
Dependent Claim
77 words
A process for treating a person whose bone marrow has been destroyed or lost its functional ability, said process comprising the steps of: I. obtaining a bone marrow sample from a donor; and then II. replicating the bone marrow sample in vitro to produce a replicated bone marrow containing hematopoietic stem cells having marrow repopulating activity; and then III. infusing the replicated bone marrow containing hematopoietic stem cells into the person to restore hematopoiesis in the person.
Claim 3
Dependent Claim
22 words
The process of claim 1 further comprising the step of cryopreserving the bone marrow sample prior to replicating the bone marrow sample.
Claim 4
Dependent Claim
25 words
The process of claim 1 wherein the bone marrow sample is obtained by aspirating a bone marrow sample from the iliac crest of the donor.
Claim 5
Dependent Claim
21 words
The process of claim 1 wherein the donor of Step I and the person of Step III are the same person.
Claim 6
Dependent Claim
20 words
The process of claim 1 wherein the donor of Step I and the person of Step III are different persons.
Claim 7
Dependent Claim
45 words
The process of claim 1 wherein the bone marrow sample is replicated in vitro by growing bone marrow cells of the bone marrow sample, including hematopoietic stem cells, in a culture containing secretory products of normal marrow stromal cells and secretory products of extramedullary macrophages.
Claim 8
Dependent Claim
33 words
The process of claim 6 further comprising the step of plating the bone marrow cells on a dish containing a reticular fiber network prior to growing the bone marrow cells in the culture.
Claim 9
Dependent Claim
18 words
The process of claim 7 further comprising the step of subsetting fibroblasts to produce the reticular fiber network.
Claim 10
Dependent Claim
34 words
The process of claim 6 further comprising the step of producing the secretory products of extramedullary macrophages by growing extramedullary macrophage cells in the presence of prostaglandins E 2 , E 1 and A.
Claim 11
Dependent Claim
14 words
The process of claim 9 wherein the extramedullary macrophages are hepatic and splenic macrophages.
Claim 12
Dependent Claim
18 words
The process of claim 9 wherein the extramedullary macrophage cells are grown in the presence of latex beads.
Claim 13
Dependent Claim
30 words
The process of claim 6 further comprising the step of producing the secretory products of marrow stromal cells by growing marrow stromal cells in the presence of prostaglandins and interleukins.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-01-25 | 1991-07-27 | 1992-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-01-25 | 1995-07-27 | 1996-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-01-25 | 1999-07-27 | 2000-01-26 | $7700.00 | Window Closed / Lapsed |