US4402934A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic technique for rheumatoid arthritis
Assignee: Individual
•
Filing: 1980-10-30
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Granted: 1983-09-06
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Expires: 2000-10-30
Abstract
Abstract A method for diagnosis of rheumatoid arthritis and related diseases comprises determination of polyclonal lymphocyte activation in B-cells cultured in the presence of patient serum. A diagnostic kit is also provided.
Inventors
- 👤 Marius C. Teodorescu
- 👤 John L. Skosey
- 👤 Jin-Lai Chang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; G01N2800/00; G01N2800/10; G01N2800/101; G01N2800/102; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
73 words
A method for diagnosing rheumatoid arthritis and related diseases in a human patient, comprising the steps of: (a) providing a lymphocyte population, comprising B-cells, suspended in a culture medium; (b) adding to the B-cell suspension with mixing a portion of serum taken from said patient; (c) cultivating in vitro the resulting mixture of serum and B-cell suspension; and (d) determining the extent of polyclonal lymphocyte activation exhibited by the B-cells in said mixture.
Claim 2
Independent Claim
73 words
A method for diagnosing rheumatoid arthritis and related diseases in a human patient, comprising the steps of: (a) providing a lymphocyte population, comprising B-cells, suspended in a culture medium; (b) adding to the B-cell suspension with mixing a portion of serum taken from said patient; (c) cultivating in vitro the resulting mixture of serum and B-cell suspension; and (d) determining the extent of polyclonal lymphocyte activation exhibited by the B-cells in said mixture.
Claim 3
Dependent Claim
24 words
The method of claim 1 wherein the lymphocyte population is selected from the class consisting of rabbit B-cells, human B-cells, and mouse spleen B-cells.
Claim 4
Dependent Claim
27 words
The method of claim 1 wherein the cell suspensions are cultivated at about 37° C. for a period of from about 4 hours to about 72 hours.
Claim 5
Dependent Claim
21 words
The method of claim 1 wherein the polyclonal lymphocyte activation is determined by a measurement of the presence of surface receptors.
Claim 6
Dependent Claim
21 words
The method of claim 4 wherein the polyclonal lymphocyte activation is determined by a measurement of the presence of surface immunoglobulin.
Claim 7
Dependent Claim
23 words
The method of claim 1 wherein the extent of polyclonal lymphocyte activation is measured by incorporating a radio-labelled precursor into the B-cell culture.
Claim 8
Dependent Claim
26 words
The method of claim 1 wherein the serum taken from the subject patient is fractionated and a selected fraction thereof is added to the B-cell suspension.
Claim 9
Dependent Claim
44 words
The method of claim 1 wherein a lymphocyte population, comprising mouse B-cells, is suspended in a medium comprising normal human serum and radio-labelled precursors, mixing with the suspension a sample of patient serum, cultivating the mixture, and determining the extent of polyclonal lymphocyte activation.
Claim 10
Dependent Claim
61 words
A diagnostic kit, for assay of polyclonal lymphocyte activation of B-cells, as a determinant for rheumatoid arthritis in a subject patient, comprising: (a) a packaged suspension of B-cells in a culture medium; (b) means for adding to said suspension a sample of patient serum, or fraction thereof; and (c) means for determining, after incubation, the proportion of cells having surface immunoglobulin.
Claim 11
Dependent Claim
61 words
A diagnostic kit, for assay of polyclonal lymphocyte activation of B-cells, as a determinant for rheumatoid arthritis in a subject patient, comprising: (a) a packaged suspension of B-cells in a culture medium; (b) means for adding to said suspension a sample of patient serum, or fraction thereof; and (c) means for determining, after incubation, the proportion of cells having surface immunoglobulin.
Claim 12
Dependent Claim
28 words
The diagnostic kit of claim 9 wherein the packaged suspension of B-cells in a culture medium is provided as a frozen suspension maintained at substantially liquid nitrogen temperature.
References Cited (Backward Citations)
US4314987A
Cited
US4302437A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-09-05 | 1987-03-07 | 1987-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-09-05 | 1991-03-07 | 1991-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-09-05 | 1995-03-07 | 1995-09-06 | $7700.00 | Window Closed / Lapsed |