Cell growth promoting material

Claim 2

A process for preparing growth promoting material, whose effectiveness can be demonstrated by in vitro cell culture experiments, which comprises: (a) slowly contacting serum or plasma with sufficient chilled perchloric acid to reach a 0.1 to 0.25 final molar concentration of said perchloric acid in said serum or plasma, (b) at a temperature of -1° C. to 15° C., (c) under intensive mixing which is continued until a homogeneous suspension is obtained, (d) separating the resultant precipitate, which contains the growth-promoting substances, from the supernatant, (e) eluting said growth-promoting substances from said precipitate by first resuspending said precipitate in an aqueous alkaline or salt solution, and thereafter, (f) adjusting the pH to solubilize the growth-promoting substances from the insoluble proteins, (g) separating the supernatant, which contains the growth-promoting substances, from the insoluble, undesired precipitate, (h) exchanging the solvent in the supernatant for a physiological solution, and (i) sterilizing the resultant growth-promoting material.

Claim 3

The process of claim 1 wherein serum is utilized in step (a).

Claim 4

The process of claim 2 wherein the serum is bovine serum.

Claim 5

The process of claim 1 wherein plasma is utilized in step (a).

Claim 6

A composition comprising material suitable for promoting cell growth, whose effectiveness can be demonstrated by in vitro cell culture experiments in both human and animal-derived cell types at a level comparable to the activity of Fetal Bovine Serum, said material prepared by a process which comprises: (a) slowly contacting serum or plasma, derived from non-fetal bovine blood, with sufficient chilled perchloric acid to reach a 0.1 to 0.25 final molar concentration of said perchloric acid in said serum or plasma, (b) at a temperature of -1° C. to 15° C., (c) under intensive mixing which is continued until a homogeneous suspension is obtained, (d) separating the resultant precipitate, which contains the growth-promoting substances, from the supernatant, (e) eluting said growth-promoting substances from said precipitate by first resuspending said precipitate in an aqueous alkaline or salt solution, and thereafter, (f) adjusting the pH to solubilize the growth-promoting substances from the insoluble proteins, (g) separating the supernatant, which contains the growth-promoting substances, from the insoluble, undesired precipitate, (h) exchanging the solvent in the supernatant for a physiological solution, and (i) sterilizing the resultant growth-promoting material.

Claim 7

A composition comprising material suitable for promoting cell growth, whose effectiveness can be demonstrated by in vitro cell culture experiments in both human and animal-derived cell types at a level comparable to the activity of Fetal Bovine Serum, said material prepared by a process which comprises: (a) slowly contacting serum or plasma, derived from non-fetal bovine blood, with sufficient chilled perchloric acid to reach a 0.1 to 0.25 final molar concentration of said perchloric acid in said serum or plasma, (b) at a temperature of -1° C. to 15° C., (c) under intensive mixing which is continued until a homogeneous suspension is obtained, (d) separating the resultant precipitate, which contains the growth-promoting substances, from the supernatant, (e) eluting said growth-promoting substances from said precipitate by first resuspending said precipitate in an aqueous alkaline or salt solution, and thereafter, (f) adjusting the pH to solubilize the growth-promoting substances from the insoluble proteins, (g) separating the supernatant, which contains the growth-promoting substances, from the insoluble, undesired precipitate, (h) exchanging the solvent in the supernatant for a physiological solution, and (i) sterilizing the resultant growth-promoting material.

Claim 8

The composition of claim 5 wherein serum is utilized in step (a).

Claim 9

The composition of claim 5 wherein plasma is utilized in step (a).