US4226747A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunological diagnostic reagents comprising thio-amine terminated latex particles
Assignee: Roche Diagnostics Corp
•
Filing: 1978-07-31
•
Granted: 1980-10-07
•
Expires: 1998-07-31
Abstract
Abstract A reagent for an immunological determination comprising water insoluble latex particles being formed from vinyl polymerizates and having free terminal functional groups of the formula: <IMAGE> through which are bound an immunologically active material including materials for preparing this reagent and for utilizing this reagent for diagnostic purposes. The latex particles contain a core and an outer layer, said core being formed by polymerization of vinyl, or diene monomers or mixtures thereof with said polymerizates in said core carrying a functional group selected from group consisting of carboxyl, sulfonate or mixtures thereof and said outer layer being formed from vinyl polymerizates carrying said terminal functional group.
Inventors
- 👤 Gaetano Roncari
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; Y; Y10; Y10S; Y10S436/00; Y10S436/823
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
113 words
A reagent for an immunological determination comprising a water insoluble reagent in the form of discrete latex particles, said reagent having a specific weight corresponding to about that of water, said latex particles being formed from vinyl polymerizates and having free terminal functional groups of the formula ##STR7## through which is bound an immunologically active material, wherein said latex particles contain a core and an outer layer, said core containing polymerizates formed from vinyl or dienes monomers or mixtures thereof, with said polymerizates in said core carrying a functional group selected from the group consisting of carboxyl, sulfonate or mixtures thereof and said outer layer containing vinyl polymerizates carrying said terminal funtional groups.
Claim 2
Dependent Claim
113 words
A reagent for an immunological determination comprising a water insoluble reagent in the form of discrete latex particles, said reagent having a specific weight corresponding to about that of water, said latex particles being formed from vinyl polymerizates and having free terminal functional groups of the formula ##STR7## through which is bound an immunologically active material, wherein said latex particles contain a core and an outer layer, said core containing polymerizates formed from vinyl or dienes monomers or mixtures thereof, with said polymerizates in said core carrying a functional group selected from the group consisting of carboxyl, sulfonate or mixtures thereof and said outer layer containing vinyl polymerizates carrying said terminal funtional groups.
Claim 3
Dependent Claim
22 words
The reagent of claim 1, wherein said latex particles have an average diameter of from about 0.03 μm to about 5 μm.
Claim 4
Dependent Claim
56 words
The reagent of claim 2, wherein the vinyl polymerizates are homopolymerizates of monomers selected from the group consisting of vinyl chloride, vinylidene chloride, vinyl acetate, acryl derivatives, styrene and styrene derivatives, copolymerizates of said monomers with one another or copolymerizates of said monomers with modified vinyl comonomers which constitute up to 5 wt.% of the copolymerizate.
Claim 5
Dependent Claim
53 words
The reagent of claim 2 wherein the diene polymerizates are homopolymerizates of a diene monomer selected from the group consisting of butadiene and its derivatives or copolymerizates of said diene monomers with one another, with vinyl monomers, with modified vinyl comonomers constituting up to 5 wt.% of the copolymerizate, or with mixtures thereof.
Claim 6
Dependent Claim
65 words
The reagent of claim 3, wherein the vinyl polymerizates which form the outer layer are homopolymerizates of olefin monomers selected from the group consisting of styrene, styrene derivatives, alkyl acrylates, alkyl methacrylates, acrylonitrile and methacrylonitrile, or copolymerizates of said olefin monomers, or copolymerizates of said olefin monomers with modified acting vinyl comonomers wherein the said vinyl comonomers constitute up to 5 wt.% of the copolymerizates.
Claim 7
Dependent Claim
46 words
A reagent according to any one of claim 2, wherein the core polymerizate comprises from about 30 wt.% to about 99.5 wt.% of the latex particle and the polymerizate of the outer layer constitutes from about 70 wt.% to about 0.5 wt.% of the latex particle.
Claim 8
Dependent Claim
25 words
The reagent of claim 1, wherein the amount of immunologically active material is from about 0.01 wt.% to about 15.0 wt.% based upon the latex.
Claim 9
Dependent Claim
23 words
The reagent of claim 7, wherein the immunologically active material is bound covalently to said terminal functional groups on the discrete latex particles.
Claim 10
Dependent Claim
14 words
The reagent of claim 1, wherein the immunologically active material is human immunoglobulin G.
Claim 11
Dependent Claim
16 words
The reagent of claim 1, wherein the immunologically active material is goat anti-human albumin immunoglobulin G.
Claim 12
Dependent Claim
16 words
The reagent of claim 1, wherein the immunologically active material is sheep anti-human IgG immunoglobulin G.
Claim 13
Dependent Claim
23 words
The reagent of claim 1, wherein the immunologically active material is bound to the latex through the diazotization of the terminal functional group.
Claim 14
Dependent Claim
37 words
The reagent of claim 1 wherein the immunologically active material is bound to the latex by means of a bifunctional compound which reacts with the free terminal functional group of the latex and the immunologically active material.
Claim 15
Dependent Claim
101 words
A process for preparing a reagent for immunological determination comprising reacting discrete latex particles formed from vinyl polymerizates and having a specific weight corresponding to about that of water and having free terminal functional group of the formula: ##STR8## with an immunologically active material, wherein said latex particles contain a core and an outer layer, said core formed by polymerization of vinyl, or diene monomers or mixtures thereof with said polymerizates in said core carrying a functional group selected from group consisting of carboxyl, sulfonate or mixtures thereof and said outer layer formed from vinyl polymerizates carrying said terminal functional group.
Claim 16
Dependent Claim
101 words
A process for preparing a reagent for immunological determination comprising reacting discrete latex particles formed from vinyl polymerizates and having a specific weight corresponding to about that of water and having free terminal functional group of the formula: ##STR8## with an immunologically active material, wherein said latex particles contain a core and an outer layer, said core formed by polymerization of vinyl, or diene monomers or mixtures thereof with said polymerizates in said core carrying a functional group selected from group consisting of carboxyl, sulfonate or mixtures thereof and said outer layer formed from vinyl polymerizates carrying said terminal functional group.
Claim 17
Dependent Claim
20 words
The process of claim 14 wherein the latex particles have a diameter of about 0.03 μm to about 5 μm.
Claim 18
Dependent Claim
23 words
The process of claim 15 wherein the immunologically active material is bound to the latex through the diazotization of said function terminal group.
Claim 19
Dependent Claim
14 words
The process of claim 16 wherein said immunologically active material is denatured immunoglobulin G.
Claim 20
Dependent Claim
16 words
The process of claim 15 wherein the immunologically active material is goat anti-human albumin immonoblogulin G.
Claim 21
Dependent Claim
24 words
The process of claim 15 wherein the immunologically active material is bound to said terminal functional groups of said latex through a bifunctional compound.
Claim 22
Dependent Claim
14 words
The process of claim 19 wherein said bifunction compound is p-phenyl diisothiocyanate or bis-diazobenzidine.
References Cited (Backward Citations)
DE1806418A1
Cited
DE2204684A1
Cited
US3857931A
Cited
US4118349A
Cited
US4059685A
Cited
BE845402A
Cited
US4064080A
Cited
FR2331567A1
Cited
US4140662A
Cited
US4134872A
Cited
GB2001996A
Cited
US4156669A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-07 | 1984-04-07 | 1984-10-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-07 | 1988-04-07 | 1988-10-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-07 | 1992-04-07 | 1992-10-07 | $7700.00 | Window Closed / Lapsed |