Patent US3813525A successfully refreshed from Google Patents.
US4172071A
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Chromophore blue adsorbent method for the purification of preparations having an interferon type activity
Assignee: Bpifrance Financement SA
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Filing: 1977-05-19
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Granted: 1979-10-23
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Expires: 1997-05-19
Abstract
Abstract The invention relates to a process for the purification of preparations having an interferon type-activity which comprises contacting the crude preparations with an adsorbent comprising as ligand a chromophore blue, equilibrated with an aqueous buffer whose concentration in salts is isomolar or hypomolar as compared with that of blood, and separating the fixed products having an interferon activity by using an aqueous buffer whose concentration in salts is hypermolar as compared with that of blood.
Inventors
- 👤 Edward De Maeyer
- 👤 Jaqueline De Maeyer nee Guignard
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 21:59
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/565; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
160 words
A process for the separation of products having an interferon-type activity, starting from a preparation containing said interferon-like product comprising contacting said preparation with an adsorbent, equilibrated with the aid of a buffer whose concentration in salt is isomolar or hypomolar as compared with that of blood, said adsorbent comprising on the one hand a gel forming a solid support the size of whose pores is such that it permits the passage of macromolecules, and on the other hand a compound fixed upon the said support, playing the role of ligand vis-a-vis the products to be separated, and possessing the polycyclic structure of the blue chromophore, sold under the trade mark CIBACRON BLUE F3GA, of formula ##STR2## whose configuration makes it possible to retain the interferon in a selective manner, the quantity of adsorbent used and the period of contact between the ligand and the preparation being both sufficient to enable the desired fixation of the products with interferon-type activity.
Claim 2
Dependent Claim
160 words
A process for the separation of products having an interferon-type activity, starting from a preparation containing said interferon-like product comprising contacting said preparation with an adsorbent, equilibrated with the aid of a buffer whose concentration in salt is isomolar or hypomolar as compared with that of blood, said adsorbent comprising on the one hand a gel forming a solid support the size of whose pores is such that it permits the passage of macromolecules, and on the other hand a compound fixed upon the said support, playing the role of ligand vis-a-vis the products to be separated, and possessing the polycyclic structure of the blue chromophore, sold under the trade mark CIBACRON BLUE F3GA, of formula ##STR2## whose configuration makes it possible to retain the interferon in a selective manner, the quantity of adsorbent used and the period of contact between the ligand and the preparation being both sufficient to enable the desired fixation of the products with interferon-type activity.
Claim 3
Dependent Claim
42 words
A process according to claim 1, for obtaining purified preparations having an interferon-type activity, characterized by the supplementary step of desorbing the fixed products with the help of a buffer whose concentration in salts is hyper-molar as compared with that of blood.
Claim 4
Dependent Claim
15 words
A process according to claim 1, characterized in that the solid support comprises a polysaccharide.
Claim 5
Dependent Claim
28 words
A process according to claim 3, characterized by the fact that the ligand, coupled on to the solid support, is CIBACRON BLUE F3GA or CIBACRON BLUE 3GA chromophore.
Claim 6
Dependent Claim
26 words
A process according to claim 4, characterized by the fact that said chromophore is attached to a high molecular weight polysaccharide known under the designation DEXTRAN.
Claim 7
Dependent Claim
42 words
A process according to claim 1, characterized by the fact that said adsorbent comprises an agarose to which is attached said chromophore blue via the intermediary of a polysaccharide, and which is the product sold under the trade mark BLUE DEXTRAN SEPHAROSE.
Claim 8
Dependent Claim
31 words
A process according to claim 1, characterized by the fact that the concentration in salts of the buffer used to equilibrate the adsorbent is less than or equal to 0.15 M.
Claim 9
Dependent Claim
24 words
A process according to claim 2, characterized by the fact that the concentration in salts of the desorption buffer is at least 0.5 M.
Claim 10
Dependent Claim
49 words
A process for obtaining purified preparations having an interferon-type activity, comprising contacting a complex of products having an interferon-type activity on an adsorbent according to claim 1 with an aqueous solution of a buffer whose concentration in salts is at least 0.5 M, whereby the fixed products are desorbed.
Claim 11
Dependent Claim
50 words
A process according to claim 1 characterized by the fact that the contact of said adsorbent and of the preparations to be purified on the one hand and the contact of the fixed products with said buffer on the other hand is carried on in a column of affinity chromatography.
Claim 12
Dependent Claim
20 words
A process according to claim 1 characterized by the fact that it is carried on with a fibroblast interferon preparations.
Claim 13
Dependent Claim
20 words
A process according to claim 1 characterized by the fact that it is carried on with a leucocyte interferon preparation.
Claim 14
Dependent Claim
24 words
A process according to claim 3 wherein the polysaccharide is a modified agarose in which the polysaccharide chains are cross-linked into a three-dimensional sieve.
Claim 15
Dependent Claim
17 words
A process according to claim 3 wherein the polysaccharide is an agarose sold under the trademark SEPHAROSE.
Claim 16
Dependent Claim
25 words
A process according to claim 5 wherein said polysaccharide has a molecular weight of about 2 million and is known under the designation DEXTRAN 2,000.
Claim 17
Dependent Claim
13 words
Products with an interferon-type activity fixed on the adsorbent according to claim 1.
Claim 18
Dependent Claim
20 words
A process according to claim 2 characterized by the fact that it is carried on with a fibroblast interferon preparation.
Claim 19
Dependent Claim
20 words
A process according to claim 2 characterized by the fact that it is carried on with a leucocyte interferon preparation.
Claim 20
Dependent Claim
49 words
A process according to claim 2 characterized by the fact that the contact of said adsorbent and the preparation to be purified on the one hand and the contact of the fixed product with the buffer on the other hand is carried out in a column of affinity chromatography.
Claim 21
Dependent Claim
17 words
A process according to claim 1 wherein the solid support comprises a polyacrylamide or an acrylic resin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-10-22 | 1983-04-23 | 1983-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-10-22 | 1987-04-23 | 1987-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-10-22 | 1991-04-23 | 1991-10-23 | $7700.00 | Window Closed / Lapsed |