US3975344A
Expired / Lapsed
US
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Interferon purification
Assignee: GD Searle LLC
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Filing: 1974-10-02
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Granted: 1976-08-17
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Expires: 1994-10-02
Abstract
Abstract Interferon and interferon-like proteins isolated from animal cells are purified by means of zonal density gradient centrifugation.
Inventors
- 👤 Alan Asher Schwartz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:38
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/826; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
31 words
A method for the purification of interferon or interferon-like proteins isolated from animal cells which comprises subjecting crude interferon or interferon-like proteins isolated from animal cells to zonal density gradient centrifugation.
Claim 2
Independent Claim
31 words
A method for the purification of interferon or interferon-like proteins isolated from animal cells which comprises subjecting crude interferon or interferon-like proteins isolated from animal cells to zonal density gradient centrifugation.
Claim 3
Dependent Claim
25 words
The method of claim 1, wherein the interferon or interferon-like proteins isolated from animal cells is human interferon or interferon-like proteins isolated from animal cells.
Claim 4
Dependent Claim
25 words
The method of claim 1, wherein the interferon or interferon-like proteins isolated from animal cells is monkey interferon or interferon-like proteins isolated from animal cells.
Claim 5
Dependent Claim
11 words
The method of claim 1, wherein the gradient material is sucrose.
Claim 6
Dependent Claim
12 words
The method of claim 1, wherein the gradient material is cesium chloride.
Claim 7
Dependent Claim
21 words
The method of claim 1, wherein the interferon or interferon-like proteins isolated from animal cells is derived from human fibroblast material.
Claim 8
Dependent Claim
36 words
The method of claim 1, wherein human interferon or interferon-like proteins isolated from animal cells is purified by zonal density gradient centrifugation at 48,000 rpm for 41 hours at 5°C. and the gradient material is sucrose.
Claim 9
Dependent Claim
40 words
The method of claim 1, wherein monkey interferon or interferon-like proteins isolated from animal cells is purified by zonal density gradient centrifugation at 48,000 rpm for 48 hours at 5°C. and the gradient material is 0 - 25% cesium chloride.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-17 | 1980-02-16 | 1980-08-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-17 | 1984-02-16 | 1984-08-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-17 | 1988-02-16 | 1988-08-17 | $7700.00 | Window Closed / Lapsed |