Selective removal of albumin from blood fluid and compositions therefore

Claim 1

A method of removing albumin from substantially cell free aqueous fluids containing the same comprising the steps of a. suspending a composition of the general formula [SP] x - [CRD] wherein CRD is color reactive dye of the general formula ##STR15## wherein R 3 is hydrogen, alkyl, phenyl, alkoxy phenyl, alkyl phenyl, or ##STR16## wherein R 1 is hydrogen, an alkali metal, or an alkaline earth metal, R 2 is halo or -O-dextran Z 1 is two hydrogen atoms, or --CH=CH--CH=CH-- to form a naphthyl nucleus with the phenyl nucleus to which it is attached, Z 2 is one bond of a nitrogen-nitrogen bond or a hydrogen attached to the nitrogen shown only, Q is substituted or unsubstituted aryl where Z 2 is hydrogen, or Q is substituted or unsubstituted aryl-N= where Z 2 is one bond of a double bond, m is 1 or 0, n is 1 or 2, provided that the substituent groups aforesaid are other than halo or heavy metal wherein heavy metal is defined as a metal falling in periodic groups other than 2A, 3A or 4A. and wherein SP is a solid support phase selected from the group comprising agarose, polyacrylamide and acrylic resin containing amino groups and x is expressed as a weight ratio between 0.8 and 40,

Claim 2

A method of removing albumin from substantially cell free aqueous fluids containing the same comprising the steps of a. suspending a composition of the general formula [SP] x - [CRD] wherein CRD is color reactive dye of the general formula ##STR15## wherein R 3 is hydrogen, alkyl, phenyl, alkoxy phenyl, alkyl phenyl, or ##STR16## wherein R 1 is hydrogen, an alkali metal, or an alkaline earth metal, R 2 is halo or -O-dextran Z 1 is two hydrogen atoms, or --CH=CH--CH=CH-- to form a naphthyl nucleus with the phenyl nucleus to which it is attached, Z 2 is one bond of a nitrogen-nitrogen bond or a hydrogen attached to the nitrogen shown only, Q is substituted or unsubstituted aryl where Z 2 is hydrogen, or Q is substituted or unsubstituted aryl-N= where Z 2 is one bond of a double bond, m is 1 or 0, n is 1 or 2, provided that the substituent groups aforesaid are other than halo or heavy metal wherein heavy metal is defined as a metal falling in periodic groups other than 2A, 3A or 4A. and wherein SP is a solid support phase selected from the group comprising agarose, polyacrylamide and acrylic resin containing amino groups and x is expressed as a weight ratio between 0.8 and 40,

Claim 22

A method of removing albumin from substantially cell free aqueous fluids containing the same comprising the steps of a. suspending a composition of the general formula [SP] x --[CRD] wherein CRD is a color reactive dye of the general formula ##STR19## wherein R 3 is lower alkyl, phenyl, lower alkoxy phenyl, alkyl phenyl, ##STR20## and hydrogen wherein R 1 is hydrogen, an alkali metal, or an alkaline earth metal, R 2 is halo or --O-- dextran Z 1 is [-H] 2 or --CH=CH--CH=CH-- Z 2 is nitrogen-nitrogen bond or a hydrogen attached to the nitrogen shown only, Q is substituted or unsubstituted aryl where Z 2 is hydrogen, or substituted or unsubstituted aryl-N= where Z 2 is a double bond, provided that Q is selected from substituted and unsubstituted members of the group consisting of phenyl, naphthyl, phenyl azo phenyl and anthraquinonyl wherein the substituents are selected from the group consisting of sulpho and the alkali metal salts thereof, hydroxy, amino, alkanoylamido and aralkanoyl amido wherein the alkanoyl moiety contains 1-5 carbon atoms, and wherein SP is a solid support phase selected from the group comprising agarose, polyacrylamide and acrylic resin containing amino groups and x is expressed as a weight ratio between 0.8 and 40, in a buffer of at least 0.05M molarity at a pH of between 6.5 and 8.5, b. Contacting said suspension with said albumin containing fluid to provide a suspension having albumin adsorbed upon the solid phase thereof, c. Separating the solid phase-albumin adsorbate from the aqueous phase.

Claim 23

A method of removing albumin from substantially cell free aqueous fluids containing the same comprising the steps of a. suspending a composition of the general formula [SP] x --[CRD] wherein CRD is a color reactive dye of the general formula ##STR19## wherein R 3 is lower alkyl, phenyl, lower alkoxy phenyl, alkyl phenyl, ##STR20## and hydrogen wherein R 1 is hydrogen, an alkali metal, or an alkaline earth metal, R 2 is halo or --O-- dextran Z 1 is [-H] 2 or --CH=CH--CH=CH-- Z 2 is nitrogen-nitrogen bond or a hydrogen attached to the nitrogen shown only, Q is substituted or unsubstituted aryl where Z 2 is hydrogen, or substituted or unsubstituted aryl-N= where Z 2 is a double bond, provided that Q is selected from substituted and unsubstituted members of the group consisting of phenyl, naphthyl, phenyl azo phenyl and anthraquinonyl wherein the substituents are selected from the group consisting of sulpho and the alkali metal salts thereof, hydroxy, amino, alkanoylamido and aralkanoyl amido wherein the alkanoyl moiety contains 1-5 carbon atoms, and wherein SP is a solid support phase selected from the group comprising agarose, polyacrylamide and acrylic resin containing amino groups and x is expressed as a weight ratio between 0.8 and 40, in a buffer of at least 0.05M molarity at a pH of between 6.5 and 8.5, b. Contacting said suspension with said albumin containing fluid to provide a suspension having albumin adsorbed upon the solid phase thereof, c. Separating the solid phase-albumin adsorbate from the aqueous phase.