Patent US3380446A successfully refreshed from Google Patents.
Patent US6008292A successfully refreshed from Google Patents.
US6008292A
Expired / Lapsed
US
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Method for inhibiting calcification of aldehyde-fixed bioprosthetic materials
Assignee: Edwards Lifesciences Corp
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Filing: 1997-12-02
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Granted: 1999-12-28
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Expires: 2017-12-02
Abstract
Abstract A method for preparing collagenous biological tissues for use as implantable bioprosthetic material, and articles prepared thereby. The method generally comprises the steps of a) contacting the tissue with an aldehyde fixative to effect cross-linking of the connective tissue protein(s) and subsequently b) contacting the tissue with a polyglycidyl ether. The treatment of the crosslinked tissue with the polyglycidyl ether serves to mitigate the tissue's propensity to undergo calcification following implantation in the body.
Inventors
- 👤 Catherine Ting Lee
- 👤 Jun Yang
- 👤 Tan Thanh Dinh
- 👤 Ernest H. Pfadenhauer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 22:50
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3695; A61L27/14; A61L27/22; A61L27/24; A61L27/3604; A61L27/3687; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; A61L2400/00; A61L2400/02; A61L2430/00; A61L2430/40
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
50 words
A method for mitigating calcification of a collagenous bioprosthetic material which has previously been crosslinked with an aldehyde corsslinking agent, said method comprising the step of: a. after the collagenous bioprosthetic material has been crosslinked by the aldehyde crosslinking agent, contacting the crosslinked collagenous bioprosthetic material with a polyglycidyl ether.
Claim 2
Independent Claim
50 words
A method for mitigating calcification of a collagenous bioprosthetic material which has previously been crosslinked with an aldehyde corsslinking agent, said method comprising the step of: a. after the collagenous bioprosthetic material has been crosslinked by the aldehyde crosslinking agent, contacting the crosslinked collagenous bioprosthetic material with a polyglycidyl ether.
Claim 3
Dependent Claim
31 words
The method of claim 1 wherein the polyglycidyl ether used in Step a is selected from the group of polyglycidyl ethers consisting of: ethylene glycol diglycidyl ether; and glycerol polyglycidyl ether.
Claim 4
Dependent Claim
28 words
The method of claim 1 wherein Step a comprises: contacting the bioprosthetic material with a solution containing approximately 1-10 percent by volume of at least one polyglycidyl ether.
Claim 5
Dependent Claim
26 words
The method of claim 1 wherein Step a comprises: contacting the bioprosthetic material with a solution containing approximately 1-10 percent by volume ethylene glycol diglycidyl ether.
Claim 6
Dependent Claim
25 words
The method of claim 1 wherein Step a comprises: contacting the bioprosthetic material with a solution containing approximately 1-10 percent by volume glycerol polyglycidyl ether.
Claim 7
Dependent Claim
35 words
The method of claim 1 wherein Step a comprises: contacting the bioprosthetic material with a solution containing approximately 1-10 percent by volume ethylene glycol diglycidyl ether in combination with approximately 10-30 percent by volume ethanol.
Claim 8
Dependent Claim
34 words
The method of claim 4 wherein Step a comprises: contacting the bioprosthetic material with a solution containing approximately 1-10 percent by volume glycerol polyglycidyl ether in combination with approximately 10-30 percent by volume ethanol.
Claim 9
Dependent Claim
22 words
The method of claim 1 wherein step a comprises: contacting the bioprosthetic material with a mixture containing at least two polyglycidyl ethers.
Claim 10
Dependent Claim
25 words
The method of claim 8 wherein step a comprises: contacting the bioprosthetic material with a mixture of ethylene glycol diglycidyl ether and glycerol polyglycidyl ether.
Claim 11
Independent Claim
65 words
A method for preparing a fixed biological tissue which contains connective tissue protein, said method comprising the steps of: a. providing a biological tissue which contains at least one connective tissue protein; b. contacting the biological tissue with an aldehyde fixative to effect crosslinking of the connective tissue protein (s) within the tissue; and, thereafter, c. contacting the aldehyde-fixed biological tissue with a polyglycidyl ether.
Claim 12
Independent Claim
65 words
A method for preparing a fixed biological tissue which contains connective tissue protein, said method comprising the steps of: a. providing a biological tissue which contains at least one connective tissue protein; b. contacting the biological tissue with an aldehyde fixative to effect crosslinking of the connective tissue protein (s) within the tissue; and, thereafter, c. contacting the aldehyde-fixed biological tissue with a polyglycidyl ether.
Claim 13
Dependent Claim
27 words
The method of claim 10 wherein step (b) comprises: contacting the biological tissue with a solution of approximately 0.625% glutaraldehyde buffered to pH 7.4 with phosphate buffer.
Claim 14
Dependent Claim
16 words
The method of claim 10 wherein steps (b) and (c) are carried out in separate containers.
Claim 15
Dependent Claim
20 words
The method of claim 10 wherein the method further comprises rinsing of the biological tissue between steps (b) and (c).
Claim 16
Dependent Claim
57 words
The method of claim 10 wherein step (b) is performed by immersing the biological tissue in a first solution containing an aldehyde fixative for a period of time sufficient to effect aldehyde crosslinking of the tissue and thereafter, step (c) is performed by adding a polyglycidyl ether to the first solution wherein the biological tissue is immersed.
Claim 17
Dependent Claim
42 words
The method of claim 10 wherein steps (b) and (c) are carried out by initially immersing the biological tissue in a an aldehyde solution and thereafter adding at least one polyglycidyl ether to the glutaraldehyde solution to form the aldehyde/polyglycidyl ether admixture.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-12-27 | 2003-06-28 | 2003-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-12-27 | 2007-06-28 | 2007-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-12-27 | 2011-06-28 | 2011-12-28 | $7700.00 | Window Closed / Lapsed |