US5994123A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Sugarcane bacilliform virus promoter
Assignee: University of Minnesota Twin Cities
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Filing: 1996-08-09
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Granted: 1999-11-30
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Expires: 2016-08-09
Abstract
Abstract An isolated and purified DNA molecule comprising a sugarcane bacilliform virus promoter and expression cassettes comprising said promoter are provided. Also provided is a method of using a sugarcane bacilliform virus promoter to express proteins, RNA transcripts, or mixtures thereof, in transgenic plants.
Inventors
- 👤 Neil Olszewski
- 👤 Iris Tzafrir
- 👤 David A. Somers
- 👤 Benham Lockhart
- 👤 Kimberly Torbert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:53
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N2730/00; C12N2730/00011; C12N2730/00022
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
52 words
An isolated and purified DNA segment comprising a sugarcane bacilliform virus promoter selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5, or a biologically active variant or fragment thereof which initiates RNA transcription, wherein the DNA segment does not encode a sugarcane bacilliform virus polypeptide.
Claim 2
Dependent Claim
52 words
An isolated and purified DNA segment comprising a sugarcane bacilliform virus promoter selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5, or a biologically active variant or fragment thereof which initiates RNA transcription, wherein the DNA segment does not encode a sugarcane bacilliform virus polypeptide.
Claim 3
Dependent Claim
11 words
The DNA segment of claim 1 which comprises SEQ ID NO:3.
Claim 4
Dependent Claim
11 words
The DNA segment of claim 1 which comprises SEQ ID NO:4.
Claim 5
Dependent Claim
11 words
The DNA segment of claim 1 which comprises SEQ ID NO:5.
Claim 6
Dependent Claim
63 words
A chimeric expression cassette comprising a first DNA segment which comprises a sugarcane bacilliform virus promoter selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5, or a biologically active variant or fragment thereof which initiates RNA transcription, functional in a host cell, operably linked to a second DNA segment encoding a protein or an RNA transcript.
Claim 7
Dependent Claim
63 words
A chimeric expression cassette comprising a first DNA segment which comprises a sugarcane bacilliform virus promoter selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5, or a biologically active variant or fragment thereof which initiates RNA transcription, functional in a host cell, operably linked to a second DNA segment encoding a protein or an RNA transcript.
Claim 8
Dependent Claim
26 words
The expression cassette of claim 5 further comprising a third DNA segment encoding a chloroplast transit peptide which is operably linked to the second DNA segment.
Claim 9
Dependent Claim
15 words
The expression cassette of claim 5 wherein the first DNA segment comprises SEQ ID NO:3.
Claim 10
Dependent Claim
15 words
The expression cassette of claim 5 wherein the first DNA segment comprises SEQ ID NO:4.
Claim 11
Dependent Claim
15 words
The expression cassette of claim 5 wherein the first DNA segment comprises SEQ ID NO:5.
Claim 12
Dependent Claim
12 words
The expression cassette of claim 5 which further comprises an enhancer element.
Claim 13
Dependent Claim
20 words
The expression cassette of claim 5 wherein the second DNA segment comprises a selectable marker gene or a reporter gene.
Claim 14
Dependent Claim
16 words
The expression cassette of claim 5 wherein the promoter is constitutively expressed in the host cell.
Claim 15
Dependent Claim
14 words
The expression cassette of claim 5 wherein the host cell is a plant cell.
Claim 16
Dependent Claim
14 words
The expression cassette of claim 13 wherein the host cell is a dicot cell.
Claim 17
Dependent Claim
14 words
The expression cassette of claim 13 wherein the host cell is a monocot cell.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-11-29 | 2003-05-31 | 2003-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-11-29 | 2007-05-31 | 2007-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-11-29 | 2011-05-31 | 2011-11-30 | $7700.00 | Window Closed / Lapsed |