US5378619A
Expired / Lapsed
US
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Promoter for transgenic plants
Assignee: Monsanto Technology LLC
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Filing: 1993-12-22
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Granted: 1995-01-03
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Expires: 2013-12-22
Abstract
Abstract A full-length transcript promoter from figwort mosaic virus (FMV) is identified and its DNA sequence given. The promoter functions as a strong and uniform promoter for chimeric genes inserted into plant cells. This strong promoter function is exhibited by a histochemical assay in floral buds and by reproductive scores of transgenic plants including the promoter. The promoter preferably includes a 5' leader sequence that may be from the FMV itself or from a heterologous source with respect to the promoter. The promoter is used in a plant cassette vector, a chimeric gene and in methods for transforming plant cells to obtain transgenic plants, plant cells or seeds incorporating the FMV promoter.
Inventors
- 👤 Stephen G. Rogers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:42
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N2730/00; C12N2730/00011; C12N2730/00022
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
53 words
An isolated DNA molecule comprising a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said DNA molecule is free of DNA sequence encoding figwort mosaic virus viral proteins and wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11.
Claim 2
Dependent Claim
53 words
An isolated DNA molecule comprising a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said DNA molecule is free of DNA sequence encoding figwort mosaic virus viral proteins and wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11.
Claim 3
Dependent Claim
17 words
A DNA molecule of claim 1 further comprising a 5' non-translated leader sequence from figwort mosaic virus.
Claim 4
Dependent Claim
22 words
A DNA molecule of claim 1 further comprising a 5' non-translated leader sequence from a source heterologous with respect to said promoter.
Claim 5
Dependent Claim
46 words
In a method for transforming a plant cell to express a chimeric gene, the improvement comprising a chimeric gene containing a DNA promoter sequence that directs transcription of downstream heterologous structural genes in plants, said promoter sequence comprising nucleotides 11 through 573 SEQ ID NO. 11.
Claim 6
Dependent Claim
46 words
In a method for transforming a plant cell to express a chimeric gene, the improvement comprising a chimeric gene containing a DNA promoter sequence that directs transcription of downstream heterologous structural genes in plants, said promoter sequence comprising nucleotides 11 through 573 SEQ ID NO. 11.
Claim 7
Dependent Claim
18 words
A method of claim 4 wherein said chimeric gene comprises a non-translated leader sequence from figwort mosaic virus.
Claim 8
Dependent Claim
23 words
A method of claim 4 wherein said chimeric gene comprises a non-translated leader sequence from a source heterologous with respect to said promoter.
Claim 9
Dependent Claim
104 words
A chimeric gene that functions in plant cells comprising operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to the promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 10
Dependent Claim
104 words
A chimeric gene that functions in plant cells comprising operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to the promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 11
Dependent Claim
16 words
The chimeric gene of claim 7 wherein said non-translated leader sequence is from figwort mosaic virus.
Claim 12
Dependent Claim
21 words
The chimeric gene of claim 7 wherein said non-translated leader sequence is from a source heterologous with respect to the promoter.
Claim 13
Dependent Claim
105 words
A transformed plant cell that contains a chimeric gene comprising operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to said promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 14
Dependent Claim
105 words
A transformed plant cell that contains a chimeric gene comprising operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to said promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 15
Dependent Claim
17 words
A transformed plant cell of claim 10 wherein said non-translated leader sequence is from figwort mosaic virus.
Claim 16
Dependent Claim
22 words
A transformed plant cell of claim 10 wherein said non-translated leader sequence is from a source heterologous with respect to the promoter.
Claim 17
Dependent Claim
124 words
A plant transformation vector which comprises a disarmed plant tumor inducing plasmid of Agrobacterium tumefaciens that inserts a chimeric gene into susceptible plant cells, wherein said chimeric gene comprises operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to said promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 18
Dependent Claim
124 words
A plant transformation vector which comprises a disarmed plant tumor inducing plasmid of Agrobacterium tumefaciens that inserts a chimeric gene into susceptible plant cells, wherein said chimeric gene comprises operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to said promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 19
Dependent Claim
17 words
A plant transformation vector of claim 13 wherein said non-translated leader sequence is from figwort mosaic virus.
Claim 20
Dependent Claim
22 words
A plant transformation vector of claim 13 wherein said non-translated leader sequence is from a source heterologous with respect to the promoter.
Claim 21
Dependent Claim
105 words
A transgenic plant which contains a chimeric gene which comprises operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to said promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 22
Dependent Claim
105 words
A transgenic plant which contains a chimeric gene which comprises operatively linked in sequence in the 5' to 3' direction: a) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; b) a non-translated leader sequence; c) a structural DNA sequence that is heterologous with respect to said promoter; and d) a non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3' end of the RNA.
Claim 23
Dependent Claim
16 words
A transgenic plant of claim 16 wherein said non-translated leader sequence is from figwort mosaic virus.
Claim 24
Dependent Claim
22 words
A transgenic plant of claim 16 wherein said non-translated leader sequence is from a source heterologous with respect to said promoter sequence.
Claim 25
Independent Claim
141 words
A method for providing enhanced gene expression in transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 26
Independent Claim
141 words
A method for providing enhanced gene expression in transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 27
Dependent Claim
39 words
A method according to claim 19 wherein said nontranslated DNA sequence is selected from the group consisting of nopaline synthase genes, the soybean 7S storage protein genes, and the pea small subunit of the ribulose 1,5-bisphosphate carboxylase-oxygenase E9 gene.
Claim 28
Independent Claim
144 words
A method for providing enhanced gene expression in the leaves of transgenic plants which comprises operatively linked in sequence in the 5' to 3' direction: (a) transforming plant cell with a DNA molecule which comprises: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 29
Independent Claim
144 words
A method for providing enhanced gene expression in the leaves of transgenic plants which comprises operatively linked in sequence in the 5' to 3' direction: (a) transforming plant cell with a DNA molecule which comprises: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 30
Dependent Claim
40 words
A method according to claim 21 wherein said non-translated DNA sequence is selected from the group consisting of the nopaline synthase gene, the soybean 7S storage protein genes, and the pea small subunit of the ribulose 1,5-bisphosphate carboxylase-oxygenase E9 gene.
Claim 31
Independent Claim
144 words
A method for providing enhanced gene expression in the flowers of transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 32
Independent Claim
144 words
A method for providing enhanced gene expression in the flowers of transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 33
Dependent Claim
40 words
A method according to claim 23 wherein said non-translated DNA sequence is selected from the group consisting of the nopaline synthase gene, the soybean 7S storage protein genes, and the pea small subunit of the ribulose 1,5-bisphosphate carboxylase-oxygenase E9 gene.
Claim 34
Independent Claim
144 words
A method for providing enhanced gene expression in the stems of transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 35
Independent Claim
144 words
A method for providing enhanced gene expression in the stems of transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (c) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 36
Dependent Claim
40 words
A method according to claim 25 wherein said non-translated DNA sequence is selected from the group consisting of the nopaline synthase gene, the soybean 7S storage protein genes, and the pea small subunit of the ribulose 1,5-bisphosphate carboxylase-oxygenase E9 gene.
Claim 37
Independent Claim
144 words
A method for providing enhanced gene expression in the roots of transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (d) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 38
Independent Claim
144 words
A method for providing enhanced gene expression in the roots of transgenic plants which comprises: (a) transforming plant cells with a DNA molecule which comprises operatively linked in sequence in the 5' to 3' direction: (i) a DNA promoter sequence from figwort mosaic virus that directs transcription of downstream heterologous structural genes in plants, wherein said promoter sequence comprises nucleotides 11 through 573 of SEQ ID NO. 11; (ii) a non-translated leader sequence; (iii) a structural DNA sequence which is heterologous to said promoter; (iv) a non-translated DNA sequence which functions in plant cells to cause the termination of transcription and the addition of polyadenylated ribonucleotides to the 3' end of the RNA sequence; (b) selecting said plant cells which have been transformed; (d) regenerating said plant cells to provide a differentiated plant; and (d) selecting a transformed plant which expresses said structural gene.
Claim 39
Dependent Claim
40 words
A method according to claim 27 wherein said non-translated DNA sequence is selected from the group consisting of the nopaline synthase gene, the soybean 7S storage protein genes, and the pea small subunit of the ribulose 1,5-bisphosphate carboxylase-oxygenase E9 gene.
References Cited (Backward Citations)
US4940835A
Cited
EP0426641A2
Cited
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