US5164316A
Expired / Lapsed
US
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DNA construct for enhancing the efficiency of transcription
Assignee: Monsanto Technology LLC
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Filing: 1989-08-17
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Granted: 1992-11-17
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Expires: 2009-08-17
Abstract
Abstract Novel transcription initiation regions that provide for enhanced transcription of a DNA sequence, particularly a plant sequence, are provided.
Inventors
- 👤 Joan C. McPherson
- 👤 Robert Kay
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:46
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
63 words
A plant cell comprising: a DNA construct having as components, (a) a duplicated CaMV 35s enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (b) a nucleotide sequence of interest for transcription to mRNA; and (c) a termination region wherein said components are operably joined.
Claim 2
Dependent Claim
63 words
A plant cell comprising: a DNA construct having as components, (a) a duplicated CaMV 35s enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (b) a nucleotide sequence of interest for transcription to mRNA; and (c) a termination region wherein said components are operably joined.
Claim 3
Dependent Claim
24 words
The plant cell according to claim 1, wherein said promoter is a T-DNA gene 7 or gene 5 promoter or a CaMV 35S promoter.
Claim 4
Dependent Claim
15 words
The plant cell according to claim 1, further comprising as component the right T-DNA border.
Claim 5
Dependent Claim
27 words
The plant cell according to claim 1, wherein said sequence of interest is an open reading frame with an initiation codon for expressing a protein of interest.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-17 | 1996-05-18 | 1996-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-17 | 2000-05-18 | 2000-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-17 | 2004-05-18 | 2004-11-17 | $7700.00 | Window Closed / Lapsed |