US5863531A
Expired / Lapsed
US
⏱️ 0.0 years remaining
In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
Assignee: Smith and Nephew Wound Management La Jolla
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Filing: 1995-06-07
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Granted: 1999-01-26
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Expires: 2015-06-07
Abstract
Abstract A stromal cell-based three-dimensional cell culture system is provided which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. The stromal cells along with connective tissue proteins naturally secreted by the stromal cells attach to and substantially envelope a framework composed of a biocompatible non-living material formed into a three-dimensional structure having interstitial spaces bridged by the stromal cells. Living stromal tissue so formed provides support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture and/or cultures implanted in vivo. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts in vivo, which can be utilized in the body as a corrective tissue. The three-dimensional cultures can be used to form tubular tissue structures, like those of the gastrointestinal and genitourinary tracts, as well as blood vessels; tissues for hernia repair and/or tendons and ligaments.
Inventors
- 👤 Gail K. Naughton
- 👤 Brian A. Naughton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:44
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61L; A61L27/00; A61L27/36; A61L27/38; A61L27/3804; A61B; A61B90/00; A61F; A61F2/00; A61F2/02; A61K35/28; A61L27/3604; A61L27/3629; A61L27/3637; A61L27/3641; A61L27/3839; A61L27/3843; A61L27/386; A61L27/3886; A61L27/50; A61L27/60; C; C12; C12N; C12N11/00; C12N5/00; C12N5/0062; C12N5/06; C12N5/0602; C12N5/0652; C12N5/066; C12N5/067; C12N5/0671; C12N5/0676; C12N5/0677; C12N5/0684; C12N5/069; C12N5/0691; C12N5/0697; C12N5/0698; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/5082; G01N33/5088; A61B10/00; A61B10/02; A61B10/0233; A61B10/025; A61B2010/0258; A61F2/0063; A61F2/04; A61F2/06; A61F2/08; A61K2035/124; C12N2500/00; C12N2500/30; C12N2500/34; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/30; C12N2501/33; C12N2501/335; C12N2501/38; C12N2501/39; C12N2502/00; C12N2502/09; C12N2502/094; C12N2502/13; C12N2502/1323; C12N2502/28; C12N2503/00; C12N2503/04; C12N2510/00; C12N2533/00; C12N2533/30; C12N2533/40; C12N2533/70; G01N2500/00
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
44 words
A tubular living stromal tissue prepared in vitro, comprising stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 2
Dependent Claim
44 words
A tubular living stromal tissue prepared in vitro, comprising stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 3
Dependent Claim
15 words
The tubular living stromal tissue of claim 1 in which the stromal cells are fibroblasts.
Claim 4
Dependent Claim
17 words
The tubular living stromal tissue of claim 1 in which the stromal cells are human dermal fibroblasts.
Claim 5
Dependent Claim
53 words
The tubular living stromal tissue of claim 1 in which the stromal cells are fibroblasts, pericytes, macrophages, monocytes, plasma cells, mast cells, adipocytes, umbilical cord cells, bone marrow cells from umbilical cord blood, smooth muscle cells, mucosal cells from genitourinary tract, mucosal cells from the nasopharyngeal lining, endothelial cells or a combination thereof.
Claim 6
Dependent Claim
18 words
The tubular living stromal tissue of claim 1 in which the framework is composed of a biodegradable material.
Claim 7
Dependent Claim
25 words
The tubular living, stromal tissue of claim 5 in which the biodegradable material is polyglycolic acid, cotton, cat gut sutures, cellulose, gelatin, collagen or polyhydroxyalkanoates.
Claim 8
Dependent Claim
18 words
The tubular living stromal tissue of claim 1 in which the framework is composed of a non-biodegradable material.
Claim 9
Dependent Claim
34 words
The tubular living stromal tissue of claim 7 in which the non-biodegradable material is a polyamide, a polyester, a polystyrene, a polypropylene, a polyacrylate, a polyvinyl, a polycarbonate, a polytetrafluorethylene, or a nitrocellulose compound.
Claim 10
Dependent Claim
23 words
The tubular living stromal tissue of claim 1, 2, 3, 4, 5, 6, 7 or 8 in which the framework is a mesh.
Claim 11
Dependent Claim
20 words
A method for implantation of a tubular living stromal tissue, comprising implanting the tubular living stromal tissue of claim 1.
Claim 12
Independent Claim
67 words
A method for preparing a tubular living stromal tissue in vitro, comprising culturing stromal cells previously inoculated onto a three-dimensional tubular framework composed of a biocompatible, non-living material in a culture medium, so that the stromal cells and connective tissue proteins secreted by the stromal cells attach to and substantially envelope the framework to produce a three-dimensional tubular structure having interstitial spaces bridged by the stromal cells.
Claim 13
Independent Claim
67 words
A method for preparing a tubular living stromal tissue in vitro, comprising culturing stromal cells previously inoculated onto a three-dimensional tubular framework composed of a biocompatible, non-living material in a culture medium, so that the stromal cells and connective tissue proteins secreted by the stromal cells attach to and substantially envelope the framework to produce a three-dimensional tubular structure having interstitial spaces bridged by the stromal cells.
Claim 14
Dependent Claim
12 words
The method of claim 11 in which the stromal cells are fibroblasts.
Claim 15
Dependent Claim
14 words
The method of claim 11 in which the stromal cells are human dermal fibroblasts.
Claim 16
Dependent Claim
56 words
The method of claim 11 in which the stromal cells are fibroblasts, pericytes, macrophages, monocytes, plasma cells, mast cells, adipocytes, umbilical cord cells, bone marrow cells from umbilical cord blood, smooth muscle cells, mucosal cells from the gastrointestinal tract, mucosal cells from genitourinary tract, mucosal cells from the nasopharyngeal lining, endothelial cells or a combination thereof.
Claim 17
Dependent Claim
15 words
The method of claim 11 in which the framework is composed of a biodegradable material.
Claim 18
Dependent Claim
22 words
The method of claim 15 in which the biodegradable material is polyglycolic acid, cotton, cat gut sutures, cellulose, gelatin, collagen or polyhydroxyalkanoates.
Claim 19
Dependent Claim
15 words
The method of claim 11 in which the framework is composed of a non-biodegradable material.
Claim 20
Dependent Claim
31 words
The method of claim 17 in which the non-biodegradable material is a polyamide, a polyester, a polystyrene, a polypropylene, a polyacrylate, a polyvinyl, a polycarbonate, a polytetrafluorethylene, or a nitrocellulose compound.
Claim 21
Dependent Claim
20 words
The method of claim 11, 12, 13, 14, 15, 16, 17, or 18 in which the framework is a mesh.
Claim 22
Dependent Claim
16 words
The method of claim 11 further comprising culturing parenchymal cells inoculated onto the living stromal tissue.
Claim 23
Dependent Claim
47 words
The method of claim 20 in which the parenchymal cells comprise smooth muscle cells, endothelial cells, mucosal cells from gastrointestinal tract, mucosal cells from genitourinary tract, mucosal cells from the nasopharyngeal lining, or endothelial cells, fibroblasts, umbilical cord cells, or bone marrow cells from umbilical cord blood.
Claim 24
Dependent Claim
15 words
The method of claim 11 in which the culture medium is kept under static conditions.
Claim 25
Dependent Claim
20 words
The method of claim 11 in which the culture medium is kept in dynamic state by recirculating the culture medium.
Claim 26
Dependent Claim
58 words
A three-dimensional tubular blood vessel tissue, comprising endothelial cells cultured on a tubular living stromal tissue prepared in vitro, which stromal tissue comprises stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 27
Dependent Claim
58 words
A three-dimensional tubular blood vessel tissue, comprising endothelial cells cultured on a tubular living stromal tissue prepared in vitro, which stromal tissue comprises stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 28
Dependent Claim
60 words
A three-dimensional tubular gastrointestinal epithelial tissue, comprising gastrointestinal mucosal epithelial cells cultured on a tubular living stromal tissue prepared in vitro, which stromal tissue comprises stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 29
Dependent Claim
60 words
A three-dimensional tubular gastrointestinal epithelial tissue, comprising gastrointestinal mucosal epithelial cells cultured on a tubular living stromal tissue prepared in vitro, which stromal tissue comprises stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 30
Dependent Claim
59 words
A three-dimensional tubular genitourinary epithelial tissue, comprising genitourinary epithelial cells cultured on a tubular living stromal tissue prepared in vitro, which stromal tissue comprises stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 31
Dependent Claim
59 words
A three-dimensional tubular genitourinary epithelial tissue, comprising genitourinary epithelial cells cultured on a tubular living stromal tissue prepared in vitro, which stromal tissue comprises stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a three-dimensional tubular framework composed of a biocompatible, non-living material having interstitial spaces bridged by the stromal cells.
Claim 32
Independent Claim
76 words
A method for preparing a tubular living stromal tissue in vitro, comprising culturing stromal cells previously inoculated onto a three-dimensional framework composed of a biocompatible, non-living material in a culture medium, so that the stromal cells and connective tissue proteins secreted by the stromal cells attach to and substantially envelope the framework to produce a three-dimensional structure having interstitial spaces bridged by the stromal cells; and rolling the three-dimensional structure into a tubular living stromal tissue.
Claim 33
Independent Claim
76 words
A method for preparing a tubular living stromal tissue in vitro, comprising culturing stromal cells previously inoculated onto a three-dimensional framework composed of a biocompatible, non-living material in a culture medium, so that the stromal cells and connective tissue proteins secreted by the stromal cells attach to and substantially envelope the framework to produce a three-dimensional structure having interstitial spaces bridged by the stromal cells; and rolling the three-dimensional structure into a tubular living stromal tissue.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-01-25 | 2002-07-27 | 2003-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-01-25 | 2006-07-27 | 2007-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-01-25 | 2010-07-27 | 2011-01-26 | $7700.00 | Window Closed / Lapsed |