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US4963489A
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Three-dimensional cell and tissue culture system
Assignee: Advanced BioHealing Inc
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Filing: 1988-09-08
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Granted: 1990-10-16
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Expires: 2008-09-08
Abstract
Abstract The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts, grown to subconfluence on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.
Inventors
- 👤 Gail K. Naughton
- 👤 Brian A. Naughton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 01:45
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0697; C12N5/0698; A; A61; A61B; A61B90/00; A61L; A61L27/00; A61L27/36; A61L27/38; A61L27/50; A61L27/60; C12N5/0062; C12N5/0602; C12N5/0652; C12N5/0669; C12N5/067; C12N5/0671; C12N5/0676; C12N5/0677; A61B10/00; A61B10/02; A61B10/0233; A61B10/025; A61B2010/0258; A61K; A61K35/00; A61K35/12; A61K2035/124; C12N2500/00; C12N2500/30; C12N2500/34; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/30; C12N2501/33; C12N2501/335; C12N2501/38; C12N2501/39; C12N2502/00; C12N2502/09; C12N2502/094; C12N2502/13; C12N2502/1323; C12N2502/28; C12N2503/00; C12N2503/04; C12N2510/00; C12N2533/00; C12N2533/30; C12N2533/40; C12N2533/70
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
47 words
A living stromal tissue prepared in vitro, comprising stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a framework composed of a biocompatible, non-living material formed into a three dimensional structure having interstitial spaces bridged by the stromal cells.
Claim 2
Dependent Claim
47 words
A living stromal tissue prepared in vitro, comprising stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a framework composed of a biocompatible, non-living material formed into a three dimensional structure having interstitial spaces bridged by the stromal cells.
Claim 3
Dependent Claim
14 words
The living stromal tissue of claim 1 in which the stromal cells are fibroblasts.
Claim 4
Dependent Claim
30 words
The living stromal tissue of claim 1 in which the stromal cells are a combination of fibroblasts and endothelial cells, pericytes, macrophages, monocytes, leukocytes, plasma cells, mast cells or adipocytes.
Claim 5
Dependent Claim
17 words
The living stromal tissue of claim 1 in which the framework is composed of a biodegradable material.
Claim 6
Dependent Claim
23 words
The living stromal tissue of claim 4 in which the biodegradable material is cotton, polyglycolic acid, cat gut sutures, cellulose, gelatin, or dextran.
Claim 7
Dependent Claim
17 words
The living stromal tissue of claim 1 in which the framework is composed of a non-biodegradable material.
Claim 8
Dependent Claim
32 words
The living stromal tissue of claim 6 in which the non-biodegradable material is a polyamide, polyester, a polystyrene, a polypropylene, a polyacrylate, a polyvinyl, a polycarbonate, a polytetrafluoroethylene, or a nitrocellulose compound.
Claim 9
Dependent Claim
19 words
The living stromal tissue of claims 4, 5, 6 or 7 in which the framework is pre-coated with collagen.
Claim 10
Dependent Claim
21 words
The living stromal tissue of claims 1, 2, 3, 4, 5, 6, or 7 in which the framework is a mesh.
Claim 11
Dependent Claim
14 words
The living stromal tissue of claim 8 in which the framework is a mesh.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-15 | 1994-04-16 | 1994-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-15 | 1998-04-16 | 1998-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-15 | 2002-04-16 | 2002-10-16 | $7700.00 | Window Closed / Lapsed |