US5160490A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Three-dimensional cell and tissue culture apparatus
Assignee: REGENEMED Inc A CALIFORNIA Corp
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Filing: 1991-02-21
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Granted: 1992-11-03
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Expires: 2011-02-21
Abstract
Abstract The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.
Inventors
- 👤 Gail K. Naughton
- 👤 Brian A. Naughton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:02
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0697; C12N5/0698; A; A61; A61B; A61B90/00; A61K; A61K35/00; A61K35/12; A61K35/28; A61L; A61L27/00; A61L27/36; A61L27/38; A61L27/50; A61L27/60; A61B10/00; A61B10/02; A61B10/0233; A61B10/025; A61B2010/0258; A61K2035/124; C12N2500/00; C12N2500/30; C12N2500/34; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/30; C12N2501/33; C12N2501/335; C12N2501/38; C12N2501/39; C12N2502/00; C12N2502/09; C12N2502/091; C12N2502/094; C12N2502/13; C12N2502/1323; C12N2503/00; C12N2503/04; C12N2510/00; C12N2533/00; C12N2533/30; C12N2533/40; C12N2533/70
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
81 words
A cytological testing apparatus, comprising a three dimensional cell culture positioned in a container to which a test substance can be added, in which the three dimensional cell culture comprises parenchymal cells cultured on a living stromal tissue prepared in vitro comprising stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a framework composed of a biocompatible, non-living material formed to a three dimensional structure having interstitial spaces bridged by the stromal cells.
Claim 2
Dependent Claim
81 words
A cytological testing apparatus, comprising a three dimensional cell culture positioned in a container to which a test substance can be added, in which the three dimensional cell culture comprises parenchymal cells cultured on a living stromal tissue prepared in vitro comprising stromal cells and connective tissue proteins naturally secreted by the stromal cells attached to and substantially enveloping a framework composed of a biocompatible, non-living material formed to a three dimensional structure having interstitial spaces bridged by the stromal cells.
Claim 3
Dependent Claim
14 words
The cytological testing apparatus of claim 1 in which the stromal cells are fibroblasts.
Claim 4
Dependent Claim
39 words
The cytological testing apparatus of claim 1 in which the stromal cells are a combination of fibroblasts and anyone or more of the following stromal cell types: endothelial cells, pericytes, macrophages, monocytes, leukocytes, plasma cells, mast cells or adipocytes.
Claim 5
Dependent Claim
17 words
The cytological testing apparatus of claim 1 in which the framework is composed of a biodegradable material.
Claim 6
Dependent Claim
23 words
The cytological testing apparatus of claim 4 in which the biodegradable material is cotton, polyglycolic acid, cat gut sutures, cellulose, gelatin or dextran.
Claim 7
Dependent Claim
17 words
The cytological testing apparatus of claim 1 in which the framework is composed of a non-biodegradable material.
Claim 8
Dependent Claim
33 words
The cytological testing apparatus of claim 6 in which the non-biodegradable material is a polyamide, a polyester, a polystyrene, a polypropylene, a polyacrylate, a polyvinyl, a polycarbonate, a polytetrafluorethylene, or a nitrocellulose compound.
Claim 9
Dependent Claim
19 words
The cytological testing apparatus of claim 4, 5, 6, or 7 in which the framework is precoated with collagen.
Claim 10
Dependent Claim
21 words
The cytological testing apparatus of claim 1, 2, 3, 4, 5, 6, or 7 in which the framework is a mesh.
Claim 11
Dependent Claim
14 words
The cytological testing apparatus of claim 8 in which the framework is a mesh.
Claim 12
Dependent Claim
22 words
The cytological testing apparatus of claim 1, 2, 3, 4, 5, 6, or 7 in which the container is a multi-welled container.
Claim 13
Dependent Claim
15 words
The cytological testing apparatus of claim 8 in which the container is a multi-welled container.
Claim 14
Dependent Claim
15 words
The cytological testing apparatus of claim 9 in which the container is a multi-welled container
Claim 15
Dependent Claim
15 words
The cytological testing apparatus of claim 1 in which the container is a multi-welled container.
Claim 16
Dependent Claim
15 words
The cytological testing apparatus of claim 1 in which the parachymal cells are hematopoietic cells.
Claim 17
Dependent Claim
16 words
The cytological testing apparatus of claim 1 in which the parachymal cells are melancocytes and keratincocytes.
Claim 18
Dependent Claim
14 words
The cytological testing apparatus of claim 1 in which the parenchymal cells are hepatocytes.
Claim 19
Dependent Claim
16 words
The cytological testing apparatus of claim 1 in which the parenchymal cells are pancreatic acinar cells.
Claim 20
Dependent Claim
15 words
The cytological testing apparatus of claim 1 in which the parenchymal cells are kidney cells.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-03 | 1996-05-04 | 1996-11-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-03 | 2000-05-04 | 2000-11-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-03 | 2004-05-04 | 2004-11-03 | $7700.00 | Window Closed / Lapsed |