Patent US5685967A successfully refreshed from Google Patents.
US5685967A
Expired / Lapsed
US
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Coated plastic mold for electrophoresis gel
Assignee: Life Technologies Corp
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Filing: 1995-12-18
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Granted: 1997-11-11
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Expires: 2015-12-18
Abstract
Abstract An improved coated plastic mold for the polymerization of electrophoresis gels. The mold controls the effects of oxygen contamination upon gel polymerization resulting in a more uniform gel with improved properties.
Inventors
- 👤 David J. Manis
- 👤 Sheldon Engelhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:38
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/453
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
78 words
A mold for an electrophoresis gel comprising: a plastic support structure having at least one interior surface defining a cavity; and an SiOx layer consisting essentially of SiOx disposed between at least a portion of said interior surface and said cavity; wherein the SiOx layer forms an oxygen barrier having an oxygen permeability of no more than 1 cc-mil/m 2 -atm-day, and the SiOx layer has an oxygen transmission rate of no more than 2 cc/m 2 -atm-day.
Claim 2
Dependent Claim
78 words
A mold for an electrophoresis gel comprising: a plastic support structure having at least one interior surface defining a cavity; and an SiOx layer consisting essentially of SiOx disposed between at least a portion of said interior surface and said cavity; wherein the SiOx layer forms an oxygen barrier having an oxygen permeability of no more than 1 cc-mil/m 2 -atm-day, and the SiOx layer has an oxygen transmission rate of no more than 2 cc/m 2 -atm-day.
Claim 3
Dependent Claim
22 words
A mold as in claim 1 wherein the SiOx layer is deposited directly onto the interior surface of said plastic support structure.
Claim 4
Dependent Claim
26 words
A mold as in claim 1 wherein the SiOx layer is deposited onto a plastic film bonded to the interior surface of said plastic support structure.
Claim 5
Dependent Claim
23 words
A medium for electrophoresis as in claim 1 wherein said SiOx layer was deposited onto said plastic support by a vacuum deposition process.
Claim 6
Dependent Claim
70 words
A mold for an electrophoresis gel as in claim 1 wherein said plastic support structure has at least one interior surface defining a slab shaped cavity; and said SiOx layer is disposed between at least a portion of said interior surface defining one surface of a slab and said cavity and between at least another portion of said interior surface defining the opposite surface of a slab and said cavity.
Claim 7
Dependent Claim
42 words
A mold for an electrophoresis gel as in claim 1 wherein said plastic support structure has at least one interior surface defining a rod shaped cavity; and said SiOx layer is disposed between substantially all of said interior surface and said cavity.
Claim 8
Dependent Claim
60 words
A mold for an electrophoresis gel as in claim 1 wherein said plastic support structure has at least one interior surface defining a cavity; and said SiOx layer is disposed between substantially all of the portion of said interior surface corresponding to those parts of a gel that may substantially contact molecules to be separated during electrophoresis, and said cavity.
Claim 9
Independent Claim
82 words
A medium for electrophoresis comprising: a plastic support structure defining a cavity; an aqueous electrophoresis gel within said cavity; and an oxygen barrier disposed between said plastic support structure and said aqueous electrophoresis gel; wherein said oxygen barrier comprises a layer including an SiOx layer consisting essentially of SiOx; and wherein said SiOx layer has an oxygen permeability no more than 0.01 cc-mil/m 2 -atm-day, and said SiOx layer has an oxygen transmission rate of no more than 2 cc/m 2 -atm-day.
Claim 10
Independent Claim
82 words
A medium for electrophoresis comprising: a plastic support structure defining a cavity; an aqueous electrophoresis gel within said cavity; and an oxygen barrier disposed between said plastic support structure and said aqueous electrophoresis gel; wherein said oxygen barrier comprises a layer including an SiOx layer consisting essentially of SiOx; and wherein said SiOx layer has an oxygen permeability no more than 0.01 cc-mil/m 2 -atm-day, and said SiOx layer has an oxygen transmission rate of no more than 2 cc/m 2 -atm-day.
Claim 11
Dependent Claim
24 words
A medium for electrophoresis as in claim 8 wherein said SiOx layer was deposited directly onto the interior surface of said plastic support structure.
Claim 12
Dependent Claim
28 words
A medium for electrophoresis as in claim 8 wherein said SiOx layer was deposited onto a plastic film bonded to the interior surface of said plastic support structure.
Claim 13
Dependent Claim
28 words
A medium for electrophoresis as in claim 8 wherein said oxygen barrier comprises a layer of inorganic oxide deposited onto said plastic support by a vacuum deposition process.
Claim 14
Independent Claim
77 words
A medium for electrophoresis comprising: a plastic support; an aqueous electrophoresis gel; an oxygen barrier disposed between said plastic support and said aqueous electrophoresis gel; wherein the permeability of said oxygen barrier is less than 1 cc-mil/m 2 -atm-day, and wherein said oxygen barrier has an oxygen transmission rate of no more than 2 cc/m 2 -atm-day; and wherein said oxygen barrier comprises an inorganic oxide selected from the group consisting of SiO and SiO 2 .
Claim 15
Independent Claim
77 words
A medium for electrophoresis comprising: a plastic support; an aqueous electrophoresis gel; an oxygen barrier disposed between said plastic support and said aqueous electrophoresis gel; wherein the permeability of said oxygen barrier is less than 1 cc-mil/m 2 -atm-day, and wherein said oxygen barrier has an oxygen transmission rate of no more than 2 cc/m 2 -atm-day; and wherein said oxygen barrier comprises an inorganic oxide selected from the group consisting of SiO and SiO 2 .
Claim 16
Dependent Claim
34 words
A medium for electrophoresis as in claim 12 wherein said oxygen barrier comprises a layer consisting essentially of one or more inorganic oxides selected from the group consisting of SiO and SiO 2 .
Claim 17
Dependent Claim
28 words
A medium for electrophoresis as in claim 12 wherein said oxygen barrier comprises a layer of inorganic oxide deposited onto said plastic support by a vacuum deposition process.
Claim 18
Dependent Claim
78 words
A medium for electrophoresis as in claim as in claim 12 wherein said plastic support has at least one interior surface defining a slab shaped cavity; said aqueous electrophoresis gel is a slab gel; and said oxygen barrier is disposed between at least a portion of said interior surface and a portion of one side of said gel and between at least another portion of said interior surface and a portion of the opposite side of said gel.
Claim 19
Dependent Claim
51 words
A medium for electrophoresis as in claim as in claim 12 wherein said plastic support structure has at least one interior surface defining a rod shaped cavity; said aqueous electrophoresis gel is a rod gel; and said oxygen barrier is disposed between substantially all of said interior surface and said gel.
Claim 20
Dependent Claim
61 words
A medium for electrophoresis as in claim as in claim 12 wherein said plastic support structure has at least one interior surface defining a cavity; and said oxygen barrier is disposed between substantially all of the portion of said interior surface corresponding to those parts of a gel that may substantially contact molecules to be separated during electrophoresis, and said gel.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-11-10 | 2001-05-12 | 2001-11-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-11-10 | 2005-05-12 | 2005-11-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-11-10 | 2009-05-12 | 2009-11-11 | $7700.00 | Window Closed / Lapsed |