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Element for electrophoresis

Assignee: DIRECTOR OF FINANCE DIVISION MINISTER'S SECRETARIAT SCIENCE AND TECHNOLOGY AGENCY
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Filing: 1984-05-18
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Granted: 1986-04-01
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Expires: 2004-05-18
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Abstract

Abstract An element for electrophoresis comprising the following three-layer structure laminated in the order: (I) a support layer; (II) an adhesive layer comprising a copolymer having at least one specifically selected repeating unit; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, and a compound containing at least one carbamoyl group (modifier).

Inventors

  • 👤 Masashi Ogawa
  • 👤 Hisashi Shiraishi
  • 👤 Teppei Ikeda

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 12:38
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31725; Y10T428/31736; Y10T428/31786; Y10T428/31797

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 555 words
An element for electrophoresis comprising the following three-layer structure laminated in the order: (I) a support layer; (II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of: (1) a repeating unit having the formula (1): ##STR22## in which R 11 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; Q 1 is --COO--, --CON(R 11 )--, or an arylene group containing 6-10 carbons atoms; L 1 is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R 11 )-- and containing 3-15 carbon atoms, or a divalent group containing at least one linkage selected from the group consisting of --O--, --N(R 11 )--, --CO--, --SO--, --SO 2 --, --SO 3 --, --SO 2 N(R 11 )--, --N(R 11 )CON(R 11 )-- and --N(R 11 )COO--, and containing 1-12 carbon atoms, in which R 11 has the same meaning as defined above; R 12 is --CH═CH 2 or --CH 2 CH 2 X 1 , in which X 1 is a substituent replaceable with a nucleophilic troup or releasable in the form of HX 1 by a base; A 1 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and x 1 , y 1 and z 1 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; (2) a repeating unit having the formula (2): ##STR23## in which R 21 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; R 22 is --CH═CH 2 or --CH 2 CH 2 X 2 , in which X 2 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 2 by a base; L 2 is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ 2 --, and --COZ 2 R 23 --, in which R 23 is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z 2 is the oxygen atom or NH; A 2 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and x 2 , y 2 and z 2 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and (3) a repeating unit having the formula (3): ##STR24## in which R 31 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; L 3 is a divalent linkage group containing 1-20 carbon atoms; X 3 is an active ester; A 3 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; x 3 , y 3 and z 3 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and m is 0 or 1; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, a water-soluble polymer, agarose and a compound containing at least one carbamoyl group serving as modifier.
Claim 2
Dependent Claim 555 words
An element for electrophoresis comprising the following three-layer structure laminated in the order: (I) a support layer; (II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of: (1) a repeating unit having the formula (1): ##STR22## in which R 11 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; Q 1 is --COO--, --CON(R 11 )--, or an arylene group containing 6-10 carbons atoms; L 1 is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R 11 )-- and containing 3-15 carbon atoms, or a divalent group containing at least one linkage selected from the group consisting of --O--, --N(R 11 )--, --CO--, --SO--, --SO 2 --, --SO 3 --, --SO 2 N(R 11 )--, --N(R 11 )CON(R 11 )-- and --N(R 11 )COO--, and containing 1-12 carbon atoms, in which R 11 has the same meaning as defined above; R 12 is --CH═CH 2 or --CH 2 CH 2 X 1 , in which X 1 is a substituent replaceable with a nucleophilic troup or releasable in the form of HX 1 by a base; A 1 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and x 1 , y 1 and z 1 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; (2) a repeating unit having the formula (2): ##STR23## in which R 21 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; R 22 is --CH═CH 2 or --CH 2 CH 2 X 2 , in which X 2 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 2 by a base; L 2 is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ 2 --, and --COZ 2 R 23 --, in which R 23 is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z 2 is the oxygen atom or NH; A 2 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and x 2 , y 2 and z 2 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and (3) a repeating unit having the formula (3): ##STR24## in which R 31 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; L 3 is a divalent linkage group containing 1-20 carbon atoms; X 3 is an active ester; A 3 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; x 3 , y 3 and z 3 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and m is 0 or 1; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, a water-soluble polymer, agarose and a compound containing at least one carbamoyl group serving as modifier.
Claim 3
Dependent Claim 20 words
The element for electrophoresis as claimed in claim 1, in which said compound serving as modifier is urea or formamide.
Claim 4
Dependent Claim 21 words
The element for electrophoresis as claimed in claim 1, in which said support layer is made of a plastic material sheet.
Claim 5
Dependent Claim 20 words
The element for electrophoresis as claimed in claim 3, in which the plastic material sheet is a polyethylene terephthalate sheet.
Claim 6
Dependent Claim 23 words
The element for electrophoresis as claimed in claim 1, in which the adhesive layer consists essentially of a polymer having the formula (1).
Claim 7
Dependent Claim 48 words
The element for electrophoresis as claimed in claim 1 in which said water-soluble polymer is contained in the amount of 2 to 100 wt.% based on the polyacrylamide solid and said agarose is contained in the aqueous polyacrylamide gel in an amount of 0.2 to 2 wt/v %.

References Cited (Backward Citations)

US4319975A Cited
US4415428A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1989-03-31 1989-09-30 1990-04-01 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1993-03-31 1993-09-30 1994-04-01 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1997-03-31 1997-09-30 1998-04-01 $7700.00 Window Closed / Lapsed

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