US5552309A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Use of polyols for improving the introduction of genetic material into cells
Assignee: Indiana University Research and Technology Corp
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Filing: 1994-09-30
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Granted: 1996-09-03
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Expires: 2014-09-30
Abstract
Abstract A process for introducing an expression vehicle (e.g., plasmids, retroviral vectors, adenoviral vectors) into cells, which comprises contacting the cells with the expression vehicle and a polyol. The polyol may be a polyoxalkylene block copolymer, such as a polyoxypropylene-polyoxyethylene block copolymer. The use of the polyol provides for greater efficiency of transduction of the expression vehicle.
Inventors
- 👤 Keith L. March
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:14
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N15/87; C12N2710/00; C12N2710/00011; C12N2710/10011; C12N2710/10311; C12N2710/10341; C12N2710/10343; C12N2710/10351; C12N2800/00; C12N2800/70; C12N2810/00; C12N2810/10
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
61 words
In a process for transfecting a cell with a viral particle, the improvement comprising: contacting said cell with a composition comprising said viral particle and a non-ionic polyol, said non-ionic polyol being a polyoxyalkylene block copolymer wherein the alkylene moieties of the polyoxyalkylene groups each have from 2 to 5 carbon atoms, whereby said cell is transfected with said viral particle.
Claim 2
Dependent Claim
61 words
In a process for transfecting a cell with a viral particle, the improvement comprising: contacting said cell with a composition comprising said viral particle and a non-ionic polyol, said non-ionic polyol being a polyoxyalkylene block copolymer wherein the alkylene moieties of the polyoxyalkylene groups each have from 2 to 5 carbon atoms, whereby said cell is transfected with said viral particle.
Claim 3
Dependent Claim
13 words
The process of claim 1 wherein said polyol is a polyoxypropylene-polyoxyethylene block copolymer.
Claim 4
Dependent Claim
24 words
The process of claim 1 wherein said polyol is present in an amount of from about 1 wt % to about 20 wt %.
Claim 5
Dependent Claim
18 words
The process of claim 3 wherein said polyol is present in an amount of about 15 wt %.
Claim 6
Dependent Claim
14 words
The process of claim 1 wherein said viral particle is an adenoviral vector particle.
Claim 7
Dependent Claim
14 words
The process of claim 1 wherein said viral particle is a retroviral vector particle.
Claim 8
Dependent Claim
24 words
The process of claim 1 wherein said polyol is present in an amount of from about 30 wt. % to about 40 wt. %.
Claim 9
Dependent Claim
11 words
The process of claim 1 wherein said cells are vascular cells.
Claim 10
Dependent Claim
44 words
A composition comprising a viral particle and a non-ionic polyol, said non-ionic polyol being a polyoxyalkylene block copolymer wherein the alkylene moieties of the polyoxyalkylene groups each have from 2 to 5 carbon atoms, and said viral particle being capable of transfecting a cell.
Claim 11
Dependent Claim
44 words
A composition comprising a viral particle and a non-ionic polyol, said non-ionic polyol being a polyoxyalkylene block copolymer wherein the alkylene moieties of the polyoxyalkylene groups each have from 2 to 5 carbon atoms, and said viral particle being capable of transfecting a cell.
Claim 12
Dependent Claim
13 words
The composition of claim 2 wherein said polyol is a polyoxypropylene-polyoxyethylene block copolymer.
Claim 13
Dependent Claim
24 words
The composition of claim 9 wherein said polyol is present in an amount of from about 1 wt % to about 20 wt %.
Claim 14
Dependent Claim
18 words
The composition of claim 11 wherein said polyol is present in an amount of about 15 wt %.
Claim 15
Dependent Claim
14 words
The composition of claim 9 wherein said viral vector is an adenoviral vector particle.
Claim 16
Dependent Claim
14 words
The composition of claim 9 wherein said viral particle is a retroviral vector particle.
Claim 17
Dependent Claim
24 words
The composition of claim 9 wherein said polyol is present in an amount of from about 30 wt. % to about 40 wt. %.
References Cited (Backward Citations)
US5298222A
Cited
US5118512A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-09-03 | 2000-03-04 | 2000-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-09-03 | 2004-03-04 | 2004-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-09-03 | 2008-03-04 | 2008-09-03 | $7700.00 | Window Closed / Lapsed |