Patent US4668224A successfully refreshed from Google Patents.
US5489261A
Expired / Lapsed
US
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Hydrogels capable of supporting cell growth
Assignee: Boston University
•
Filing: 1994-09-12
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Granted: 1996-02-06
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Expires: 2014-09-12
Abstract
Abstract A method of growing anchorage-dependent cells is disclosed in which a substrate includes a hydrogel is employed. The hydrogel is formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and includes a macromolecule capable of supporting cell growth.
Inventors
- 👤 Carl Franzblau
- 👤 Barbara A. Faris
- 👤 Linda Civerchia-Perez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:36
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/50
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
48 words
A wound dressing comprising a single layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 2
Dependent Claim
48 words
A wound dressing comprising a single layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 3
Dependent Claim
49 words
A wound dressing comprising a single-layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said single-layer hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the single-layer hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 4
Dependent Claim
49 words
A wound dressing comprising a single-layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said single-layer hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the single-layer hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 5
Dependent Claim
13 words
The wound dressing of claim 2, wherein the cell growth-supporting macromolecule is collagen.
Claim 6
Dependent Claim
13 words
The wound dressing of claim 2, wherein the cell growth-supporting macromolecule is albumin.
Claim 7
Dependent Claim
64 words
A wound dressing comprising a one layer hydrogel having incorporated therein a cell growth-supporting macromolecule, the hydrogel formed by a method comprising the steps of: a) combining a polymer of a hydrophilic monomer and the cell-growth supporting macromolecule in an aqueous solution; and b) crosslinking the polymer to gel the aqueous solution, thereby forming a hydrogel, wherein the macromolecule is incorporated within the hydrogel.
Claim 8
Dependent Claim
64 words
A wound dressing comprising a one layer hydrogel having incorporated therein a cell growth-supporting macromolecule, the hydrogel formed by a method comprising the steps of: a) combining a polymer of a hydrophilic monomer and the cell-growth supporting macromolecule in an aqueous solution; and b) crosslinking the polymer to gel the aqueous solution, thereby forming a hydrogel, wherein the macromolecule is incorporated within the hydrogel.
Claim 9
Dependent Claim
66 words
A wound dressing comprising a one-layer hydrogel having incorporated therein a cell growth-supporting macromolecule, the one-layer hydrogel formed by a method comprising the steps of: a) combining a polymer of a hydrophilic monomer and the cell-growth supporting macromolecule in an aqueous solution; and b) crosslinking the polymer to gel the aqueous solution, thereby forming the one-layer hydrogel, wherein the macromolecule is incorporated within the one-layer hydrogel.
Claim 10
Dependent Claim
66 words
A wound dressing comprising a one-layer hydrogel having incorporated therein a cell growth-supporting macromolecule, the one-layer hydrogel formed by a method comprising the steps of: a) combining a polymer of a hydrophilic monomer and the cell-growth supporting macromolecule in an aqueous solution; and b) crosslinking the polymer to gel the aqueous solution, thereby forming the one-layer hydrogel, wherein the macromolecule is incorporated within the one-layer hydrogel.
Claim 11
Dependent Claim
13 words
The wound dressing of claim 6, wherein the cell growth-supporting macromolecule is collagen.
Claim 12
Dependent Claim
13 words
The wound dressing of claim 6, wherein the cell growth-supporting macromolecule is albumin.
Claim 13
Dependent Claim
49 words
A wound dressing which is a one layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 14
Dependent Claim
49 words
A wound dressing which is a one layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 15
Dependent Claim
50 words
A wound dressing which includes a one-layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said one-layer hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the one-layer hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 16
Dependent Claim
50 words
A wound dressing which includes a one-layer hydrogel formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and having incorporated within said one-layer hydrogel a cell growth-supporting macromolecule in an amount sufficient to render the one-layer hydrogel capable of supporting growth of anchorage-dependent mammalian cells.
Claim 17
Dependent Claim
13 words
The wound dressing of claim 10, wherein the cell growth-supporting macromolecule is collagen.
Claim 18
Dependent Claim
13 words
The wound dressing of claim 10, wherein the cell growth-supporting macromolecule is albumin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1999-02-05 | 1999-08-07 | 2000-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-02-05 | 2003-08-07 | 2004-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-02-05 | 2007-08-07 | 2008-02-06 | $7700.00 | Window Closed / Lapsed |