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US5348018A
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Method for determining if tissue is malignant as opposed to non-malignant using time-resolved fluorescence spectroscopy
Assignee: Research Foundation of City University of New York
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Filing: 1991-11-25
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Granted: 1994-09-20
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Expires: 2011-11-25
Abstract
Abstract A method for determining if tissue is malignant as opposed to non-malignant (i.e., benign tumor tissue, benign tissue, or normal tissue), In one embodiment, the method comprises irradiating a human breast tissue sample with light at a wavelength of about 310 nm and measuring the time-resolved fluorescence emitted therefrom at about 340 nm. The time-resolved fluorescence profile is then compared to similar profiles obtained from known malignant and non-malignant human breast tissues. By fitting the profiles to the formula I(t)=A 1 e(-t/τ 1 )+A 2 e(-t/τ 2 ) one can quantify the differences between tissues of various conditions. For example, non-malignant human breast tissues exhibit a slow component (τ 2 ) which is less than 1.6 ns whereas malignant human breast tissues exhibit a slow component (τ 2 ) which is greater than 1.6 ns. In addition, non-malignant human breast tissues exhibit a ratio of fast to slow amplitudes (A 1 /A 2 ) which is greater than 0.85 whereas malignant human breast tissues exhibit a ratio of fast to slow amplitudes (A 1 /A 2 ) which is less than 0.6. This technique can be used with different excitation and/or emission wavelengths, and can be applied to the detection of malignancies (or other abnormal states) in tissues other than human breast tissue.
Inventors
- 👤 Robert R. Alfano
- 👤 Asima Pradhan
- 👤 Guichen C. Tang
- 👤 Leming Wang
- 👤 Yury Budansky
- 👤 Bidyut Baran Das
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:32
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6486; A; A61; A61B; A61B5/00; A61B5/0059; A61B5/0071; A61B5/0082; A61B5/0084; A61B5/0091; A61B5/43; A61B5/4306; A61B5/4312; B; B23; B23K; B23K26/00; B23K26/02; B23K26/06; B23K26/062; B23K26/0622; B23K26/0624; G01N21/6408; A61B5/0075; A61B5/0086; G01N2021/6417
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
67 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 310 nm; b) measuring a native time-resolved fluorescence emitted therefrom at a wavelength of about 340 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement.
Claim 2
Independent Claim
67 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 310 nm; b) measuring a native time-resolved fluorescence emitted therefrom at a wavelength of about 340 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement.
Claim 3
Dependent Claim
35 words
The method as claimed in claim 1 wherein said determining step comprises comparing the native time-resolved fluorescence emitted from the human breast tissue sample to that emitted from known malignment and non-malignment human breast tissues.
Claim 4
Independent Claim
126 words
A method for determining if a human breast tissue sample is malignment as opposed to non-malignment comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 310 nm; b) measuring a native time-resolved fluorescence emitted therefrom at a wavelength of about 340 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native time-resolved fluorecence emitted from the human breast tissue sample to a double exponential of the formula ##EQU1## and determining if τ 2 is less than 1.6 ns whereby the human breast tissue sample is non-malignant or greater than 1.6 ns whereby the human breast tissue sample is malignant.
Claim 5
Independent Claim
126 words
A method for determining if a human breast tissue sample is malignment as opposed to non-malignment comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 310 nm; b) measuring a native time-resolved fluorescence emitted therefrom at a wavelength of about 340 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native time-resolved fluorecence emitted from the human breast tissue sample to a double exponential of the formula ##EQU1## and determining if τ 2 is less than 1.6 ns whereby the human breast tissue sample is non-malignant or greater than 1.6 ns whereby the human breast tissue sample is malignant.
Claim 6
Independent Claim
126 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 310 nm; b) measuring a native time-resolved fluorescence emitted therefrom at a wavelength of about 340 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprising fitting a profile of the native time-resolved fluorescence emitted from the human breast tissue sample to a double exponential of the formula ##EQU2## and determining if A 1 /A 2 is greater than 0.85 whereby the human breast tissue sample is non-malignant or less than 0.6 whereby the human breast tissue sample is malignant.
Claim 7
Independent Claim
126 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 310 nm; b) measuring a native time-resolved fluorescence emitted therefrom at a wavelength of about 340 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprising fitting a profile of the native time-resolved fluorescence emitted from the human breast tissue sample to a double exponential of the formula ##EQU2## and determining if A 1 /A 2 is greater than 0.85 whereby the human breast tissue sample is non-malignant or less than 0.6 whereby the human breast tissue sample is malignant.
Claim 8
Independent Claim
81 words
A method for determining if a tissue sample selected from the group consisting of human breast tissue and human ovarian tissue is malignant as opposed to non-malignant comprising: a) irradiating the tissue sample with light at a wavelength of about 353 nm; b) measuring a native time-resolved fluorescence emitted therefrom over one or more wavelength in a spectral band from about 400 nm to about 500 nm; and c) determining the condition of the tissue sample in accordance with said measurement.
Claim 9
Independent Claim
81 words
A method for determining if a tissue sample selected from the group consisting of human breast tissue and human ovarian tissue is malignant as opposed to non-malignant comprising: a) irradiating the tissue sample with light at a wavelength of about 353 nm; b) measuring a native time-resolved fluorescence emitted therefrom over one or more wavelength in a spectral band from about 400 nm to about 500 nm; and c) determining the condition of the tissue sample in accordance with said measurement.
Claim 10
Dependent Claim
37 words
The method as claimed in claim 5 wherein said determining step comprises comparing the native time-resolved fluorescence emitted from the tissue sample to that emitted from known malignant and non-malignant tissues of the same type of tissue.
Claim 11
Dependent Claim
27 words
The method as claimed in claim 5 wherein the tissue sample is human breast tissue and wherein the native time-resolved fluorescence is measured at about 400 nm.
Claim 12
Dependent Claim
18 words
The method as claimed in claim 5 wherein the native time-resolved fluorescence is measured at about 450 nm.
Claim 13
Dependent Claim
27 words
The method as claimed in claim 5 wherein the tissue sample is human breast tissue and wherein the native time-resolved fluorescence is measured at about 500 nm.
Claim 14
Dependent Claim
16 words
The method as claimed in claim 5 wherein the tissue sample is human breast tumor tissue.
Claim 15
Independent Claim
138 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 353 nm; b) measuring a native-time resolved fluorescence emitted therefrom over one or more wavelengths in a spectral band from about 400 nm to about 500 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native time-resolved fluorescence emitted from the human breast tissue sample to a double exponential of the formula ##EQU3## and determining if τ 1 is less than about 150 ps whereby the human breast tissue sample is malignant or greater than about 200 ps whereby the human breast tissue sample is non-malignant.
Claim 16
Independent Claim
138 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 353 nm; b) measuring a native-time resolved fluorescence emitted therefrom over one or more wavelengths in a spectral band from about 400 nm to about 500 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native time-resolved fluorescence emitted from the human breast tissue sample to a double exponential of the formula ##EQU3## and determining if τ 1 is less than about 150 ps whereby the human breast tissue sample is malignant or greater than about 200 ps whereby the human breast tissue sample is non-malignant.
Claim 17
Independent Claim
135 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 353 nm; b) measuring a native time-resolved fluorescent emitted therefrom over one or more wavelengths in a spectral band from about 400 nm to about 500 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native tissue-resolved fluorescence emitted from the human breast tissue sample to a double exponential of the formula ##EQU4## and determining if τ 2 is greater than 2.4 ns whereby the human breast tissue sample is malignant or less than 2.4 ns whereby the human tissue sample is non-malignant.
Claim 18
Independent Claim
135 words
A method for determining if a human breast tissue sample is malignant as opposed to non-malignant comprising: a) irradiating the human breast tissue sample with a pulse of light at a wavelength of about 353 nm; b) measuring a native time-resolved fluorescent emitted therefrom over one or more wavelengths in a spectral band from about 400 nm to about 500 nm; and c) determining the condition of the human breast tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native tissue-resolved fluorescence emitted from the human breast tissue sample to a double exponential of the formula ##EQU4## and determining if τ 2 is greater than 2.4 ns whereby the human breast tissue sample is malignant or less than 2.4 ns whereby the human tissue sample is non-malignant.
Claim 19
Independent Claim
83 words
A method for determining if a tissue sample selected from the group consisting of human breast tissue and human lung tissue is malignant as opposed to normal, said method comprising: a) irradiating the tissue sample with light at a wavelength of about 530 nm; b) measuring a native time-resolved fluorescence emitted therefrom over tone or more wavlengths in a spectral band from about 500 nm to about 600 nm; and c) determining the condition of the tissue sample in accordance with said measurement.
Claim 20
Independent Claim
83 words
A method for determining if a tissue sample selected from the group consisting of human breast tissue and human lung tissue is malignant as opposed to normal, said method comprising: a) irradiating the tissue sample with light at a wavelength of about 530 nm; b) measuring a native time-resolved fluorescence emitted therefrom over tone or more wavlengths in a spectral band from about 500 nm to about 600 nm; and c) determining the condition of the tissue sample in accordance with said measurement.
Claim 21
Dependent Claim
37 words
The method as claimed in claim 13 wherein said determining step comprises comparing the native time-resolved fluorescence emitted from the tissue sample to that emitted from known malignment and normal tissues of the same type of tissue.
Claim 22
Dependent Claim
18 words
The method as claimed in claim 13 wherein the native time-resolved fluorescence is measured at about 600 nm.
Claim 23
Independent Claim
129 words
A method for determine if a tissue sample selected from the group consisting of malignant tissue and normal tissue is malignant comprising: a) irradiating the tissue sample with a pulse of light at a wavelength of about 530 nm; b) measuring a native time-resolved fluorescence emitted therefrom over one or more wavlengths in a spectral band from about 550 nm to about 600 nm; and c) determining the condition of the tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native time-resolved fluorescence emitted from the tissue sample to a double exponential of the formula ##EQU5## and determining if τ 1 is about 100 ps whereby the tissue sample is malignant or about 200 ps whereby the tissue sample is normal.
Claim 24
Independent Claim
129 words
A method for determine if a tissue sample selected from the group consisting of malignant tissue and normal tissue is malignant comprising: a) irradiating the tissue sample with a pulse of light at a wavelength of about 530 nm; b) measuring a native time-resolved fluorescence emitted therefrom over one or more wavlengths in a spectral band from about 550 nm to about 600 nm; and c) determining the condition of the tissue sample in accordance with said measurement wherein said determining step comprises fitting a profile of the native time-resolved fluorescence emitted from the tissue sample to a double exponential of the formula ##EQU5## and determining if τ 1 is about 100 ps whereby the tissue sample is malignant or about 200 ps whereby the tissue sample is normal.
References Cited (Backward Citations)
JPS58222331A
Cited
US4556057A
Cited
US4682020A
Cited
US4773097A
Cited
US4957114A
Cited
US4930516A
Cited
US4930516B1
Cited
US5042494A
Cited
US5137355A
Cited
US4981138A
Cited
CA2002559A1
Cited
US5115137A
Cited
US5032714A
Cited
US5039219A
Cited
US5174297A
Cited
US5131398A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1997-09-19 | 1998-03-21 | 1998-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-09-19 | 2002-03-21 | 2002-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-09-19 | 2006-03-21 | 2006-09-20 | $7700.00 | Window Closed / Lapsed |